| ABSTRACT | 第6-8页 |
| 中文摘要 | 第9-12页 |
| ABBREVIATIONS | 第12-14页 |
| CHARPTER Ⅰ A BRIEF REVIEW OF REGENERATIVE MICROENVIRONMENT AND CELL COMMUNICATIONDURING PERIPHERAL NERVE REGENERATION | 第14-41页 |
| 1.1. INTRODUCTION | 第15-16页 |
| 1.2. TNT MORPHOLOGY AND FUNCTION | 第16-24页 |
| 1.2.1. TNT Mediated Cargo Transfer | 第17-18页 |
| 1.2.2. TNTs in Stem Cells | 第18-19页 |
| 1.2.3. TNTs in Nerve Tissue Cells | 第19-20页 |
| 1.2.4. TNTs in Immune Cells | 第20-21页 |
| 1.2.5. TNTs and Cancer | 第21-22页 |
| 1.2.6. Mitochondrial Transfer by TNTs | 第22-23页 |
| 1.2.7. Mechanisms of TNTs formation | 第23-24页 |
| 1.3. INSULIN-LIKE GROWTH FACTOR-1(IGF-1)/INSULIN-LIKE GROWTH FACTOR-1 RECEPTOR(IGF-1R)SIGNALING IN PNI | 第24-32页 |
| 1.3.1. IGF-1 Signaling | 第26-28页 |
| 1.3.2. Effects of IGF-1 on Regenerating Axons and SCs | 第28-29页 |
| 1.3.3. Regulation of IGF-1 Signaling by MicroRNAs | 第29-31页 |
| 1.3.4. Therapeutic Targeting of MiRNAs Involved in The IGF-1 Signaling Pathway | 第31-32页 |
| 1.4. OUTLOOK ON REGENERATIVE MICROENVIRONMENT AND PERIPHERAL NERVE REGENERATION | 第32-33页 |
| REFERENCE | 第33-41页 |
| CHARPTER Ⅱ RAB8A/RAB11A REGULATE INTERCELLULAR COMMUNICATIONS BETWEEN NEURALCELLS VIA TUNNELING NANOTUBES | 第41-70页 |
| 2.1. Introduction | 第42-43页 |
| 2.2. Materials and Methods | 第43-49页 |
| 2.2.1. Primary SCs culture and oligonucleotide transfection | 第43-44页 |
| 2.2.2. Time-lapse imaging of live samples | 第44页 |
| 2.2.3. Scanning electron microscopy | 第44-45页 |
| 2.2.4. Quantitative real-time PCR | 第45页 |
| 2.2.5. Western Blot Analysis | 第45页 |
| 2.2.6. Sciatic nerve transection | 第45-46页 |
| 2.2.7. Immunocytochemistry | 第46页 |
| 2.2.8. Confocal Microscopy | 第46页 |
| 2.2.9. TNT quantification | 第46-47页 |
| 2.2.10. Evaluation of cell apoptosis by flow cytometry analysis | 第47页 |
| 2.2.11. TUNEL staining | 第47页 |
| 2.2.12. Wound healing assay of primary SCs in vitro | 第47-48页 |
| 2.2.13. Enzyme-linked immunosorbent assay (ELISA) | 第48页 |
| 2.2.14. Co-culture of DRG neurons and SCs | 第48-49页 |
| 2.2.15. Statistical analysis | 第49页 |
| 2.3. RESULTS | 第49-62页 |
| 2.3.1. TNTs between SCs and their properties | 第49-51页 |
| 2.3.2. TNTs facilitate intercellular transfer of lipophilic cytosolic components as well asproteins, mitochondria vesicles between SCs | 第51-53页 |
| 2.3.3. Newly-synthesized RNA is transferred from SCs to axons after sciatic nerve transectionvia TNTs | 第53-55页 |
| 2.3.4. Rab8a and Rab 11 a affect the formation of TNTs | 第55-57页 |
| 2.3.5. Endogenous Rab8a/ Rabl la are necessary for formation and function of TNTs | 第57-59页 |
| 2.3.6. Knockdown of Rab8a/Rablla promote SCs apoptosis and inhibit migration viasuppression of TNTs formation | 第59-60页 |
| 2.3.7. Rab8a/ Rab11a may affect neurotrophic factors transport and outgrowth of DRG neuronsneurites co-cultured with SCs via TNTs | 第60-62页 |
| 2.4. DISCUSSION | 第62-66页 |
| 2.5. SUMMARY AND PROSPECTIVE VIEW | 第66页 |
| Reference | 第66-70页 |
| CHARPTER Ⅲ MIR-129 CONTROLS AXONAL REGENERATION VIA REGULATING INSULIN-LIKE GROWTHFACTOR 1 IN PERIPHERAL NERVE INJURY | 第70-97页 |
| 3.1. INTRODUCTION | 第71-72页 |
| 3.2. MATERIALS AND METHODS | 第72-77页 |
| 3.2.1. Animals and tissue preparation | 第72-73页 |
| 3.2.2. Tissue immunohistochemistry | 第73页 |
| 3.2.3. DRG neuron culture | 第73-74页 |
| 3.2.4. Primary culture of SCs and cell transfection | 第74页 |
| 3.2.5. Luciferase reporter assay | 第74-75页 |
| 3.2.6. Quantitative real-time RT-PCR (qRT-PCR) | 第75-76页 |
| 3.2.7. Western blot analysis | 第76页 |
| 3.2.8. ELISA | 第76页 |
| 3.2.9. Cell proliferation assay | 第76-77页 |
| 3.2.10. Cell migration assay | 第77页 |
| 3.2.11. Co-culture of DRG neurons and SCs | 第77页 |
| 3.2.12. Statistical analysis | 第77页 |
| 3.3. RESULTS | 第77-90页 |
| 3.3.1. Temporal changes of IGF-1 expression following sciatic nerve injury | 第78-80页 |
| 3.3.2. miR-129 negatively regulated IGF-1 by directly targeting its 3'-UTR | 第80-82页 |
| 3.3.3. miR-129/IGF-1 axis regulates neurite outgrowth from DRG neurons | 第82-84页 |
| 3.3.4. miR-129 inhibited IGF-1 secretion of SCs | 第84-85页 |
| 3.3.5. miR-129 suppressed SCs proliferation and migration | 第85-88页 |
| 3.3.6. Effects of miR-129/IGF-1/IGF-1R pathway on neurite outgrowth in co-culture system ofDRG neurons and SCs | 第88-90页 |
| 3.4. DISCUSSION | 第90-92页 |
| 3.5. SUMMARY AND PROSPECTIVE VIEW | 第92-94页 |
| Reference | 第94-97页 |
| ACKNOWLEDGEMENTS | 第97-98页 |
| PUBLICATIONS | 第98-100页 |
