| 摘要 | 第7-9页 |
| ABSTRACT | 第9-10页 |
| LIST OF ABBREVIATIONS | 第11-12页 |
| CHAPTER Ⅰ. LITERATURE REVIEW | 第12-42页 |
| 1.1 General background | 第12-13页 |
| 1.2 Lipid biosynthesis | 第13-18页 |
| 1.2.1 FA biosynthesis | 第13-16页 |
| 1.2.2 Galactolipid biosynthesis in the chloroplast | 第16-17页 |
| 1.2.3 Neutral and polar lipids biosynthesis in the endoplasmic reticulum | 第17-18页 |
| 1.3 TAG storage in oil body | 第18页 |
| 1.4 The transporters involved in lipid degradation and beta oxidation | 第18-19页 |
| 1.5 Cross-membrane lipid transport and trafficking | 第19-24页 |
| 1.5.1 Flip-flop movements | 第22页 |
| 1.5.2 Plastid-ER FA transport | 第22-23页 |
| 1.5.3 ER-Plastid phospholipid and FA transport | 第23-24页 |
| 1.6 Transporters required for surface lipid deposition | 第24-29页 |
| 1.7 Role of transcriptional regulation in lipid accumulation | 第29-39页 |
| 1.7.1 Leafy cotyledon 1 (LEC1) | 第29-30页 |
| 1.7.2 Leafy cotyledon 2 (LEC2) | 第30-33页 |
| 1.7.3 Abscisic acid insensitive (ABI3) and FUSCA3 (FUS3) | 第33页 |
| 1.7.4 Wrinkled 1 (WRI1) | 第33-34页 |
| 1.7.5 Dofs increase the total FA content in transgenic plants | 第34页 |
| 1.7.6 Role of Basic leucine zipper (b ZIPs) in lipid accumulation | 第34-35页 |
| 1.7.7 MYB TFs regulate biosynthesis of VLCFAs | 第35页 |
| 1.7.8 Negative regulators of oil biosynthesis | 第35-39页 |
| 1.8 Biotechnology tools for metabolic engineering of oil yield and composition | 第39-40页 |
| 1.9 Economical importance of soybean crop | 第40-41页 |
| 1.10 Aims and objectives of the current study | 第41-42页 |
| CHAPTER Ⅱ. MATERIALS AND METHODS | 第42-49页 |
| 2.1 Identification of the soybean LAFL gene and in silico analysis | 第42页 |
| 2.2 Phylogenetic analysis | 第42页 |
| 2.3 Gene cloning | 第42-43页 |
| 2.4 Gel electrophoresis and purification of PCR product | 第43页 |
| 2.5 Arabidopsis thaliana transformation | 第43-44页 |
| 2.6 Plant growth conditions and treatment | 第44页 |
| 2.7 RNA extraction | 第44页 |
| 2.8 Quantitative RT-PCR (q RT-PCR) analysis of gene expression | 第44-45页 |
| 2.9 Genomic DNA extraction | 第45页 |
| 2.10 Plasmid extraction | 第45-46页 |
| 2.11 Seed oil extraction | 第46页 |
| 2.12 Hairy root induction | 第46页 |
| 2.13 Hairy root oil extraction | 第46-47页 |
| 2.14 Histochemical staining for starch | 第47页 |
| 2.15 Spectrophotometric determination of starch | 第47-48页 |
| 2.16 Protein extraction | 第48页 |
| 2.17 Anthocynin measurement | 第48页 |
| 2.18 CDNA library construction for Illumina deep sequencing | 第48页 |
| 2.19 Statistic analysis | 第48-49页 |
| CHAPTER Ⅲ. RESULTS | 第49-77页 |
| 3.1 Identification of LAFL genes in Glycine max genome | 第49-50页 |
| 3.2 Expression of Gm LAFL during soybean seed development | 第50-51页 |
| 3.3 Expression in Arabidopsis thaliana mutants alters seed oil production and TAG composition | 第51-53页 |
| 3.4 Ectopic expression in Arabidopsis thaliana modifies seed oil production and TAG composition | 第53-56页 |
| 3.5 GmLAFL genes alters the chemical composition of seeds | 第56-61页 |
| 3.6 GmLEAFY ectopic expression increases total protein in Arabidopsis seeds | 第61-62页 |
| 3.7 GmLEAFY genes are responsible for embryonic-vegetative phase transition | 第62-64页 |
| 3.8 Ectopic expression in soybean hairy roots promotes TAG accumulation | 第64-65页 |
| 3.9 Transcriptome analysis of over expressed soybean hairy roots | 第65-77页 |
| CHAPTER Ⅳ. DISCUSSION | 第77-84页 |
| 4.1 Each member of LAFL-clade regulate TAG content and composition | 第77-78页 |
| 4.2 GmLEC2 regulates starch and protein metabolism | 第78-79页 |
| 4.3 GmLEC2 and GmFUS3 target key regulatory genes in oil biosynthesis | 第79-81页 |
| 4.4 GmLEC2 and GmFUS3 target key genes involved in FA and TAG biosynthesis | 第81-82页 |
| 4.5 Genes involved in TAG catabolism and lipid hydrolysis are regulated by GmLEC2 and GmFUS3 | 第82-83页 |
| 4.6 GmFUS3 and GmLEC2 control the regulation of lipid transporters | 第83-84页 |
| CHAPTER Ⅴ. CONCLUSIONS | 第84-85页 |
| REFERENCES | 第85-99页 |
| APPENDIX A. LIST OF PRIMERS USED IN THIS STUDY | 第99-102页 |
| APPENDIX B. PREDICTED EXPRESSION OF LAFL GENES IN DIFFERENT TISSUES OF SOYBEAN IN PUBLIC DATABASE (PHYTOZOME) | 第102-103页 |
| APPENDIX C. PHYLOGENETIC ANALYSIS OF GMFUS3 AND GMLEC2 TARGETS IN G.MAX AND THEIR ORTHOLOGS IN OTHER CROPS | 第103-109页 |
| APPENDIX D. LIST OF TRANSCRIPTION FACTORS AND TRANPORTERS REGULATED BY GMLEC2 AND GMFUS3 IN LIPID BIOSYNTHESIS PATHWAY | 第109-111页 |
| ACKNOWLEDGEMENT | 第111-112页 |
| PUBLICATIONS | 第112-113页 |
