| ABSTRACT | 第6-7页 |
| ABBREVIATIONS AND ACRONYMS | 第8-10页 |
| INTRODUCTION | 第10-32页 |
| 1.1 General introduction | 第10-11页 |
| 1.2 Research Justification | 第11-13页 |
| 1.3 The topic review | 第13-31页 |
| 1.3.1 Simplifying procedure of hybrid rice breeding | 第14-20页 |
| 1.3.1.1 The chemically induced male sterility system | 第16页 |
| 1.3.1.2. The three-line system | 第16-17页 |
| 1.3.1.3. Two-line system | 第17-18页 |
| 1.3.1.4. One-line method | 第18-20页 |
| 1.3.2 Breeding technologies to improve yield potential of Hybrid rice | 第20-31页 |
| 1.3.2.1 Morphological improvement | 第21-23页 |
| 1.3.2.2. Enhancing Heterosis | 第23-25页 |
| 1.3.2.3. Marker Assisted selection (MAS) | 第25-28页 |
| 1.3.2.4 Biotechnology Applications | 第28-31页 |
| 1.4 Research objectives | 第31-32页 |
| 2 MATERIAL AND METHODS | 第32-42页 |
| 2.1 Rice cultivars and the mapping population | 第32-33页 |
| 2.2 Determining fertility conditions of D52S and D38S | 第33-35页 |
| 2.2.1 Microscopic observation of pollen fertility | 第33-34页 |
| 2.2.2 Visual inspection of the pollen fertility | 第34-35页 |
| 2.3 The genetic inheritance study of rPGMS gene | 第35-36页 |
| 2.3.1 Inheritance study of rpms3(t) gene based on pollen fertility | 第35页 |
| 2.3.2 Inheritance study of rPGMS gene based on seed setting rate | 第35-36页 |
| 2.4 Mapping of the rpms3(t) gene | 第36-42页 |
| 2.4.1 Harvesting of leaves | 第36页 |
| 2.4.2 DNA extraction | 第36-37页 |
| 2.4.3 Amplification of the Microsatellites | 第37-38页 |
| 2.4.4 Electrophoresis and silver staining (PAGE) | 第38页 |
| 2.4.5 Identifying potential chromosomal region containing rPGMS gene | 第38-40页 |
| 2.4.6 Linkage analysis | 第40-41页 |
| 2.4.7 Construction of genetic linkage map | 第41-42页 |
| 3 RESULTS AND ANALYSIS | 第42-57页 |
| 3.1 | 第42-47页 |
| 3.1.1 Visual inspection of pollen fertility of D52S and D38S lines | 第42-45页 |
| 3.1.2 Microscopic observation of pollen fertility | 第45-47页 |
| 3.2 Inheritance of rPGMS in D52S germplasm | 第47-52页 |
| 3.2.1 Inheritance of rPGMS based on seed setting percentages | 第47-49页 |
| 3.2.2 Inheritance of rPGMS based on pollen fertility | 第49-52页 |
| 3.2.3 Correlation analysis | 第52页 |
| 3.3 Genetic Mapping of the rpms3(t) gene | 第52-57页 |
| 3.3.1 Potential Chromosomal region containing the rPGMS Locus | 第52-55页 |
| 3.3.2 The Map Location of the rPGMS Locus | 第55-57页 |
| 4 DISCUSSION | 第57-63页 |
| 4.1 D52S and D38S are short/reverse PGMS | 第57-59页 |
| 4.2 A single recessive gene governs the inheritance of rPGMS | 第59-60页 |
| 4.3 Genetic Map of the rpms3(t)gene | 第60-63页 |
| 5 CONCLUSION AND RECOMMENDATION | 第63-65页 |
| LITERATURE CITED | 第65-77页 |
| APPENDICES | 第77-84页 |
| ACKNOWLEDGEMENT | 第84页 |
