| 摘要 | 第6-7页 |
| ABSTRACT | 第7页 |
| ABBREVIATIONS | 第13-15页 |
| Chapter 1 Introduction | 第15-44页 |
| 1.1 Importance of improvement of wheat yield | 第15-18页 |
| 1.1.1 Importance of grain weight and yield in wheat | 第17-18页 |
| 1.2 Application of molecular technology on wheat breeding | 第18-23页 |
| 1.2.1 DNA or molecular marker | 第18-20页 |
| 1.2.2 Linkage and association analysis | 第20-23页 |
| 1.3 Dissection of grain weight and yield related QTL | 第23-29页 |
| 1.3.1 Research progress in wheat grain weight and yield genes and QTL | 第24-25页 |
| 1.3.2 Grain weight and yield linked QTL | 第25-29页 |
| 1.4 Functional markers in wheat | 第29-41页 |
| 1.4.1 Functional markers (FMs) | 第29页 |
| 1.4.2 Development of FMs to isolate candidate genes from wheat genome | 第29-30页 |
| 1.4.3 Comparative genomics between wheat and rice is a good approach for dissection of wheat grain weight and yield traits | 第30-33页 |
| 1.4.4 Grain weight related genes and their functional markers | 第33-41页 |
| 1.4.5 Marker assisted selection using yield related functional markers | 第41页 |
| 1.5 Plant hormones regulating grain weight and yield | 第41-43页 |
| 1.6 Rice TGW6 gene | 第43页 |
| 1.7 The purpose of current study | 第43-44页 |
| Chapter 2 TaTGW6-Al, an ortholog of rice TGW6, is associated with grain weight and yield inbread wheat | 第44-85页 |
| 2.1 Materials and methods | 第44-51页 |
| 2.1.1 Plant materials | 第44-45页 |
| 2.1.2 Field trials for evaluation of TGW and yield | 第45页 |
| 2.1.3 Cloning and sequence analysis of TaTGW6 | 第45-46页 |
| 2.1.4 Development of a functional marker for TaTGW6-Al | 第46页 |
| 2.1.5 Data acquisition of 1000 grain weight | 第46-47页 |
| 2.1.6 Leaf DNA extraction protocol | 第47-48页 |
| 2.1.7 Protocol for purification of PCR products | 第48-49页 |
| 2.1.8 Cloning | 第49-51页 |
| 2.2 Results | 第51-80页 |
| 2.2.1 Cloning and characterization of the TaTGW6-2L and TaTGW6-7S gene in wheat | 第51-58页 |
| 2.2.2 Cloning and characterization of the TaTGW6-3L gene in wheat | 第58-80页 |
| 2.2.2.1 TaTGW6, an ortholog of rice TGW6, is associated with grain weight and yield in bread wheat | 第58-59页 |
| 2.2.2.2 Development of a dCAPS marker for TaTGW6-Al | 第59页 |
| 2.2.2.3 Linkage and QTL analysis of TaTGW6-Al | 第59-60页 |
| 2.2.2.4 Validation of dCAPS marker for TaTGW6-Al | 第60-80页 |
| 2.3 Discussion | 第80-85页 |
| 2.3.1 An accessible molecular approach to assort yield related genes in bread wheat | 第82页 |
| 2.3.2 TaTGW6-Al is a novel locus related to TGW and yield | 第82-83页 |
| 2.3.3 Development of functional marker TaTGW6-Al-CAPS | 第83页 |
| 2.3.4 Chromosome location of the functional marker for TaTGW6 | 第83-84页 |
| 2.3.5 Validation of functional marker for TaTGW6 | 第84页 |
| 2.3.6 Frequencies of allelic variants at the TaTGW6-Al locus in Chinese and foreign cultivars | 第84-85页 |
| Chapter 3 Conclusion | 第85-86页 |
| REFERENCES | 第86-103页 |
| ACKNOWLEDGEMENTS | 第103-105页 |
| CURRICULUM VITAE | 第105页 |
