| ABSTRACT | 第7-9页 |
| 摘要 | 第10-13页 |
| CHAPTER 1 Literature Review and Study Plan | 第13-23页 |
| 1.1 Dietary folic acid | 第13-16页 |
| 1.1.1 Functions of Folic Acid | 第15-16页 |
| 1.2 Fish Specific Stressors | 第16-18页 |
| 1.2.1 Capture, Transport, and Handling | 第16-17页 |
| 1.2.2 Contaminants | 第17-18页 |
| 1.2.3 Crowding | 第18页 |
| 1.3 Stress Responses and organ system | 第18-20页 |
| 1.3.1 Gills | 第18-19页 |
| 1.3.2 Liver | 第19页 |
| 1.3.3 Integument | 第19-20页 |
| 1.4 An overview of the nutrition of M. amblycephala | 第20页 |
| 1.5 Aim of the study | 第20页 |
| 1.6 Hypothesis of the study | 第20-23页 |
| CHAPTER 2 Effect of dietary Folic acid on growth performance and muscle chemical composition ofblunt snout bream M. amblycephala fingerling | 第23-33页 |
| 1 Introduction | 第23页 |
| 2 Materials and Methods | 第23-27页 |
| 2.1 Formulation and preparation of experimental diets | 第23-25页 |
| 2.2 Research facility and fish rearing | 第25页 |
| 2.3 Sampling and fish weighing | 第25-26页 |
| 2.4 Calculations | 第26页 |
| 2.5 Proximate composition analysis | 第26页 |
| 2.6 Statistical analysis | 第26-27页 |
| 3 Results | 第27-30页 |
| 3.1 Growth performance and feed utilization | 第27-28页 |
| 3.2 Effect of dietary folic acid level on the muscle proximate chemical composition | 第28-30页 |
| 4 Discussion | 第30-31页 |
| 4.1 Growth performance and feed utilization | 第30-31页 |
| 4.2 Effect of dietary folic acid level on the muscle proximate chemical composition | 第31页 |
| 5 Conclusion | 第31-33页 |
| CHAPTER 3 Effect of dietary Folic acid on blood hematological parameters and Digestive enzymesactivities of blunt snout bream fingerling M. amblycephala | 第33-39页 |
| 1 Introduction | 第33页 |
| 2 Materials and Method | 第33-35页 |
| 2.1 Formulation and preparation of experimental diets | 第33页 |
| 2.2 Research facility and fish rearing | 第33-34页 |
| 2.3 Sample collection | 第34页 |
| 2.4 Effect of dietary folic acid on Hematological measurements | 第34页 |
| 2.5 Effect of dietary folic acid on digestive enzyme activity | 第34-35页 |
| 2.6 Statistical analysis | 第35页 |
| 3 Results | 第35-36页 |
| 3.1 Effect of dietary folic acid on blood Hematological parameters | 第35页 |
| 3.2 Effect of dietary folic acid on digestive enzyme activity | 第35-36页 |
| 4 Discussion | 第36-37页 |
| 4.1 Effect of dietary folic acid on blood hematology | 第36页 |
| 4.2 Effect of dietary folic acid on digestive enzyme activity | 第36-37页 |
| 5 Conclusion | 第37-39页 |
| CHAPTER 4 Effect of dietary folic acid on biochemical parameters, Antioxidant enzymes activities andgene expression of three heat shock proteins (HSPs) in blunt snout bream Megalobrama amblycephalafingerling under acute high temperature stress | 第39-67页 |
| 1 Introduction | 第39-40页 |
| 2 Materials and Method | 第40-43页 |
| 2.1 Formulation and preparation of experimental diets | 第40页 |
| 2.2 Research facility and fish rearing | 第40页 |
| 2.3 Collection of Sample | 第40页 |
| 2.4 Biochemical measurements | 第40-41页 |
| 2.5 Immune and antioxidant parameters | 第41页 |
| 2.6 Real-time PCR analysis of hepatic HSP60, HSP70 and HSP90 | 第41-43页 |
| 2.7 Statistical analysis | 第43页 |
| 3 Results | 第43-58页 |
| 3.1 Effect of dietary folic acid level on the serum TP, TC, TG and Urea under acute hightemperature challenge | 第43-47页 |
| 3.2 Effect of dietary folic acid level on the serum Cortisol, Glucose, ACTH, ALT, AST andALP under acute high temperature challenge | 第47-51页 |
| 3.3 Effect of dietary folic acid level on the serum T3 and T4 under acute high temperaturechallenge | 第51-52页 |
| 3.4 Effect of dietary folic acid level on the serum C3,C4 and IgM under acute hightemperature challenge | 第52-54页 |
| 3.5 Effect of dietary folic on hepatic anti-oxidization enzyme activity under acute hightemperature challenge | 第54-56页 |
| 3.6 Effect of dietary folic acid on gene expression of three heat shock proteins (HSPs) underacute high temperature stress | 第56-58页 |
| 4 Discussion | 第58-66页 |
| 4.1 Effect of dietary folic acid level on the serum TP, TC, TG and Urea under acute hightemperature challenge | 第58-59页 |
| 4.2 Effect of dietary folic acid level on the serum Glucose, Cortisol, ACTH, ALT, AST andALP under acute high temperature challenge | 第59-62页 |
| 4.3 Effect of dietary folic acid level on the serum T3 and T4 under acute high temperatureStress | 第62-63页 |
| 4.4 Effect of dietary folic acid level on the serum C3,C4 and IgM under acute hightemperature challenge | 第63-64页 |
| 4.5 Effect of dietary folic on hepatic anti-oxidization enzyme activity under acute hightemperature challenge | 第64-65页 |
| 4.6 Effect of dietary folic acid on gene expression of three heat shock proteins (HSPs) underacute high temperature stress | 第65-66页 |
| 5 Conclusion | 第66-67页 |
| Conclusion of the whole study | 第67-69页 |
| References | 第69-85页 |
| Appendix | 第85-87页 |
| Acknowledgements | 第87-89页 |
| Meetings attended and publications | 第89-91页 |
| Awards | 第91页 |
