| Abbreviation | 第8-9页 |
| Abstract | 第9-11页 |
| 中文摘要 | 第12-14页 |
| Chapter 1 Literature review | 第14-45页 |
| 1. Importance and application of SCNT | 第15-18页 |
| 1.1 Reproductive cloning | 第16-17页 |
| 1.2 Therapeutic cloning | 第17-18页 |
| 2. The history of cloning | 第18-23页 |
| 2.1 The brief introduction of cloning | 第18-20页 |
| 2.2 Technical procedures of SCNT | 第20-23页 |
| 3. Efficiency of mammalian cloning and affecting factors | 第23-31页 |
| 3.1 Donor cell contribution | 第24-25页 |
| 3.2 Cytoplast source and quality | 第25-27页 |
| 3.3 Method of egg activation | 第27-31页 |
| 4. Improvement of cloning efficiency by treatment of histone deacetylaseinhibitors (HDACi) | 第31-45页 |
| 4.1 Role of epigenetics during early embryogenesis and SCNT | 第31-35页 |
| 4.2 HDACis and their application in SCNT | 第35-39页 |
| 4.3 Mechanism for Improvement of cloned embryo development byHDACis | 第39-42页 |
| 4.4. Prospectives for further HDACi study | 第42-45页 |
| Chapter 2 Experiments | 第45-110页 |
| Experiment One: Establishment of WAGYU somatic cell lines | 第46-60页 |
| 1. Materials and methods | 第46-51页 |
| 2. Results | 第51-55页 |
| 3. Discussion | 第55-58页 |
| 4. Conclusion | 第58-60页 |
| Experiment Two: Improvement of WAGYU cloning efficiency byoptimizing the cloning procedure | 第60-86页 |
| 1. Materials and Methods | 第60-70页 |
| 2. Results | 第70-78页 |
| 2.1 Effect of WAGYU donor cell lines on the development rate ofcloned embryos | 第70-72页 |
| 2.2 Effect of different cell preparation method on the developmentrate of WAGYU SCNT embryo | 第72页 |
| 2.3 The effect of demecolcine on enucleation rate of bovineoocytes | 第72-74页 |
| 2.4 The effect of nuclear transfer method on the development ofbovine cloned embryos | 第74-76页 |
| 2.5 The effect of cytoplasm amount on the development rate ofcloned WAGYU embryos | 第76-77页 |
| 2.6 The results of zone-free cloned embryo transfer | 第77-78页 |
| 3. Discussion | 第78-84页 |
| 4. Conclusion | 第84-86页 |
| Experiment Three. The effect of CUDC-101 treatment on thedevelopmental potential of WAGYU cloned embryos | 第86-110页 |
| 1. Materials and Methods | 第87-98页 |
| 2. Results | 第98-102页 |
| 2.1 The effect of different concentrations of CUDC-101 treatment of fibroblasts on the developmental potency of WAGYUcloned embryos | 第98-99页 |
| 2.2 The effect of high concentrations of CUDC-101 treatment of fibroblasts on in vitro development of WAGYU clonedembryos | 第99-100页 |
| 2.3 The effect of CUDC-101 treatment duration of fibroblasts onin vitro development of WAGYU cloned embryos | 第100页 |
| 2.4 The effect of CUDC-treatment of donor cells on geneexpressions in WAGYU cloned blastocyst | 第100-102页 |
| 3. Discussion | 第102-109页 |
| 4. Conclusion | 第109-110页 |
| Chapter 3 Conclusion | 第110-112页 |
| References | 第112-126页 |
| Acknowledgement | 第126-128页 |
| Resume | 第128页 |
