| ABSTRACT | 第4页 |
| 摘要 | 第5-7页 |
| ABBREVIATIONS | 第7-8页 |
| Chapter 1 Introduction | 第8-12页 |
| 1.1 Regenerative medicine and stem cell transplantation | 第8-9页 |
| 1.2 A brief history and timeline of hematopoietic cell transplantation | 第9-11页 |
| 1.3 The aim of the research | 第11-12页 |
| Chapter 2 Isolation and Analysis of Hematopoietic Progenitor Cells from Mouse Embryos Multiple Tissues | 第12-35页 |
| 2.1 Introduction | 第12-16页 |
| 2.1.1 Early hematopoietic development in yolk sac | 第14页 |
| 2.1.2 Early hematopoietic development in aorta-gonad mesonephros region | 第14-15页 |
| 2.1.3 Early hematopoietic development in placenta | 第15页 |
| 2.1.4 Early hematopoietic development in fetal liver | 第15-16页 |
| 2.2. Experimental Section | 第16-21页 |
| 2.2.1 Materials | 第16-17页 |
| 2.2.1.1 Mice | 第16页 |
| 2.2.1.2 Tools and reagents | 第16-17页 |
| 2.2.2 Methods | 第17-21页 |
| 2.2.2.1 Isolation hematopoietic progenitor cells and dissection of embryonic tissues | 第17-18页 |
| 2.2.2.2 Flow cytometric analysis of cells from embryonic multiple tissues | 第18页 |
| 2.2.2.3 Cell preparation for staining | 第18-19页 |
| 2.2.2.4 Staining of single cell suspension | 第19页 |
| 2.2.2.5 RT-PCR analysis | 第19-20页 |
| 2.2.2.6 Culture of hematopoietic stem/progenitor cells on artificial ECM | 第20页 |
| 2.2.2.7 Preparation of Vitronectin-coated surfaces | 第20页 |
| 2.2.2.8 Stepwisely Induction of ESCs to HSCs | 第20-21页 |
| 2.3 Results | 第21-31页 |
| 2.3.1 Ontogenic expression profile of CD34 in mouse YS | 第21页 |
| 2.3.2 Ontogenic expression profile of CD45 and CD41 in mouse YS | 第21-24页 |
| 2.3.3 Ontogenic expression profile of CD34 in mouse AGM | 第24页 |
| 2.3.4 Ontogenic expression profile of CD45 and CD41 in mouse AGM | 第24-25页 |
| 2.3.5 Ontogenic expression profile of CD34 in mouse placenta | 第25-27页 |
| 2.3.6 Ontogenic expression profile of CD45 and CD41 in mouse Placenta | 第27-30页 |
| 2.3.7 Ontogenic expression profile of CD41 and CD45 in mouse fetal liver | 第30-31页 |
| 2.4 Discussions | 第31-34页 |
| 2.5 Conclusions and perspectives | 第34-35页 |
| Chapter 3 Supplementary information | 第35-39页 |
| Chapter 4 References | 第39-44页 |
| Chapter 5 Acknowledgements | 第44-45页 |
| Chapter 6 Participated International Confere | 第45页 |
| List of Publications | 第45-46页 |
