| ACKNOWLEDGEMENTS | 第6-8页 |
| INTRODUCTION | 第8-16页 |
| ABSTRACT | 第16-17页 |
| 中文摘要 | 第18-24页 |
| ABBREVIATIONS | 第24-31页 |
| CHAPTER 1 Phoenixin-14 levels are increased in association with luteinizing hormone and nesfatin-1 levels in women with polycystic ovary syndrome | 第31-45页 |
| 1.1 Background | 第31-34页 |
| 1.2 Patients and Methods | 第34-36页 |
| 1.3 Results | 第36-42页 |
| 1.3.1 Demographical characteristics and biochemical values of control women and PCOS patients. | 第36页 |
| 1.3.2 Baseline hormone levels in control women and PCOS patients | 第36页 |
| 1.3.3 PCOS patients had significantly higher serum levels of phoenixin-14 and nesfatin-1 | 第36页 |
| 1.3.4 Possible correlations between phoenixin-14 and PCOS related reproductive and metabolic disturbances | 第36-42页 |
| 1.4 Discussion | 第42-44页 |
| 1.5 Conclusion | 第44-45页 |
| CHAPTER 2 Serum estradiol levels in controlled ovarian stimulation directly affect the endometrium: | 第45-73页 |
| 2.1 Background | 第45-48页 |
| 2.2 Materials and methods | 第48-55页 |
| 2.2.1 Patients and sample collection | 第48页 |
| 2.2.2 Cells and cell culture | 第48-49页 |
| 2.2.3 JAr spheroid-endometrial cell attachment assay | 第49页 |
| 2.2.4 Protein Extraction | 第49-50页 |
| 2.2.5 Protein digestion and iTRAQ labeling | 第50页 |
| 2.2.6 Strong cationic-exchange chromatography separation | 第50-51页 |
| 2.2.7 LC-ESI-MS/MS analysis | 第51页 |
| 2.2.8 Protein identification and quantification | 第51-52页 |
| 2.2.9 Western blotting analysis | 第52-53页 |
| 2.2.10 Tissue collection and Immunohistochemistry | 第53页 |
| 2.2.11 Bioinformatics Analysis | 第53-54页 |
| 2.2.12 Statistical analysis | 第54-55页 |
| 2.3 Results | 第55-68页 |
| 2.3.1 Effects of different concentrations of E_2 on JAr spheroid attachment to endometrial cell monolayers | 第55-57页 |
| 2.3.2 Protein expression profiles in cells treated with different concentrations of E_2 | 第57-60页 |
| 2.3.3 Validation of DEPs by western blot analysis | 第60-62页 |
| 2.3.4 Immunohistochemical localization of C3,KNG-1,PLG and ERa in the receptive endometrium | 第62页 |
| 2.3.5 Ingenuity pathway analysis (IPA) yields distinct functional Groupings | 第62-63页 |
| 2.3.6 Canonical signaling pathways of DEPs | 第63页 |
| 2.3.7 Upstream analysis | 第63-68页 |
| 2.4 Discussion | 第68-72页 |
| 2.5 Conclusions | 第72-73页 |
| CHAPTER 3 Major findings and Future perspectives | 第73-75页 |
| 3.1 Introduction | 第73-74页 |
| 3.2 Future perspectives | 第74-75页 |
| REFERENCES | 第75-92页 |
| LIST OF PUBLICATIONS | 第92页 |
