| ABSTRACT | 第10-12页 |
| LIST OF ABBREVIATIONS | 第14-16页 |
| CHAPTER 1 LITERATURE REVIEW | 第16-54页 |
| 1.1. SOYBEAN INTRODUCTION AND ITS IMPORTANCE | 第16-18页 |
| 1.1.1. Soybean | 第16-17页 |
| 1.1.2. Soybean importance | 第17-18页 |
| 1.2. SOYBEAN PRODUCTION AND ITS CONSTRAINTS | 第18-20页 |
| 1.2.1. Soybean production | 第18页 |
| 1.2.2. Constraints to soybean production | 第18-19页 |
| 1.2.3. Viruses threaten the soybean production | 第19-20页 |
| 1.3. SOYBEAN MOSAIC VIRUS, A MAJOR THREAT | 第20-29页 |
| 1.3.1. Historical background and spread of SMV | 第20-21页 |
| 1.3.2. SMV Taxonomy and Strains | 第21-22页 |
| 1.3.3. Molecular biology of SMV | 第22-24页 |
| 1.3.4. Insect vectors of SMV | 第24-25页 |
| 1.3.5. Source of inoculum and disease development | 第25-26页 |
| 1.3.6. Synergism with other viruses | 第26-27页 |
| 1.3.7. Impact of SMV infection on Soybean | 第27-29页 |
| 1.4. DISEASE MANAGEMENT | 第29-30页 |
| 1.5. HOST PLANT RESISTANCE | 第30-35页 |
| 1.5.1. The nature of host plant resistance | 第32-34页 |
| 1.5.2. Introgession of host plant resistance | 第34-35页 |
| 1.6. GENOMICS-ASSISTED BREEDING(GAB) | 第35-36页 |
| 1.7. MOLECULAR MARKERS AND GENETIC LINKAGE MAPS IN SOYBEAN | 第36-39页 |
| 1.7.1. Molecular markers resources for soybean | 第36-38页 |
| 1.7.2. Genetic linkage maps in soybean | 第38-39页 |
| 1.8. GENE MAPPING | 第39-42页 |
| 1.8.1. Bulk segregant analysis followed by traditional mapping | 第40页 |
| 1.8.2. Genotyping-by-sequencing (GBS) | 第40-42页 |
| 1.9. SOURCES, GENETICS AND GENES FOR RESISTANCE TO SMV | 第42-49页 |
| 1.9.1. Sources and genetics of resistance to SMV | 第42-44页 |
| 1.9.2. Genes for resistance to SMV | 第44-49页 |
| 1.10. RESEARCH BACKGROUND AND OBJECTIVES | 第49-54页 |
| CHAPTER 2 INHERITANCE,FINE-MAPPING AND CANDIDATE GENEANALYSIS OF THE GENE RESISTANT TO SMV STRAIN SC5 | 第54-76页 |
| 2.1. INTRODUCTION | 第54-57页 |
| 2.2. MATERIALS AND METHODS | 第57-65页 |
| 2.2.1. Plant genetic materials | 第57页 |
| 2.2.2. Source and maintenance of SMV strain | 第57页 |
| 2.2.3. Inoculation and resistance evaluation | 第57-58页 |
| 2.2.4. Statistical analysis | 第58页 |
| 2.2.5. Construction of genetic bin map and gene mapping | 第58-60页 |
| 2.2.6. Association analysis and candidate gene selection | 第60页 |
| 2.2.7. Quantitative Real Time-PCR (QRT-PCR) for expression profiling | 第60-65页 |
| 2.3. RESULTS | 第65-76页 |
| 2.3.1. Resistance spectra of Kefeng.no.1,Nannong-1138-2 and the F_1 progeny | 第65页 |
| 2.3.2. Inheritance of the resistance | 第65-66页 |
| 2.3.3. Identification of genomic region associated withresistance gene to SC5 | 第66-67页 |
| 2.3.4. Candidate genes identification | 第67-69页 |
| 2.3.5. Expression profiling of candidate genes | 第69-74页 |
| 2.3.6. Summary of results | 第74-76页 |
| CHAPTER 3 INHERITANCE, FINE-MAPPING AND CANDIDATE GENEANALYSIS OF THE GENE RESISTANT TO SMV STRAIN SC20 | 第76-108页 |
| 3.1. INTRODUCTION | 第76-78页 |
| 3.2. MATERIALS AND METHODS | 第78-94页 |
| 3.2.1. Plant materialsand source of strain | 第78-79页 |
| 3.2.2. Inoculation and phenotypic assesment | 第79页 |
| 3.2.3. DNA extractions, choice of SSR markers and mapping techniques | 第79-85页 |
| 3.2.4. Data and linkage analysis | 第85页 |
| 3.2.5. Segregating population development | 第85-86页 |
| 3.2.6. Fine-mapping and molecular markers development | 第86-87页 |
| 3.2.7. Candidate gene prediction | 第87页 |
| 3.2.8. Quantitative Real Time-PCR (QRT-PCR) for expression profiling | 第87页 |
| 3.2.9. Amplification and sequencing of resistance gene candidates | 第87-93页 |
| 3.2.10. Amino acid prediction and sequence alignment | 第93-94页 |
| 3.3. RESULTS | 第94-108页 |
| 3.3.1. Inheritance analysis of resistance to SC20 in Qihuang.No.1 | 第94-95页 |
| 3.3.2. Identification of genomic region associated with resistance gene to SC20 | 第95页 |
| 3.3.3. Delimitation of a candidate genomic region | 第95-97页 |
| 3.3.4. Candidate gene details | 第97-98页 |
| 3.3.5. Expression profiling of candidate genes | 第98-100页 |
| 3.3.6. Sequence analysis of resistance gene candidates | 第100-107页 |
| 3.3.7. Summary of results | 第107-108页 |
| CHAPTER 4 DISCUSSION | 第108-120页 |
| 4.1. NATURE OF SC5 AND SC20 RESISTANCE GENES | 第108-109页 |
| 4.2. ADVANTAGES OF THE GBS BASED BIN MAP STRATEGY | 第109页 |
| 4.3. REFINING THE GENOMIC REGION FOR SC5 RESISTANCE BY HIGHDENSITY BIN MAP | 第109-110页 |
| 4.4. SMV RESISTANCE GENES IN CHROMOSOME 2 (LG D1b) | 第110页 |
| 4.5. MOST POSSIBLE CANDIDATE GENE TO SC5 RESISTANCE | 第110-112页 |
| 4.6. STRATEGY FOR MAPPING OF SC20 RESISTANCE GENE | 第112页 |
| 4.7. SC20 RESISTANCE GENE POSITIONED AT RESISTANCEGENES RICH REGION IN CHROMOSME 13 (LG-F) | 第112-113页 |
| 4.8. COMPARISON OF SC20 RESISTANCE GENE WITH OTHERSMV RESISTANCE GENES IN CHROMOSME 13 (LG-F) | 第113-115页 |
| 4.9. MOST POSSIBLECANDIDATE GENES TO SC20 RESISTANCE | 第115-116页 |
| 4.10. CONCLUSION | 第116-120页 |
| CHAPTER 5 MAJOR INNOVATION POINTS | 第120-122页 |
| 5.1. NATURE OF SC5 AND SC20 RESISTANCE GENES | 第120页 |
| 5.2. CANDIDATE GENOMIC REGION AND MOST POSSIBLE CANDIDATE GENETO SC5 RESISTANCE | 第120-121页 |
| 5.3. CANDIDATE GENOMIC REGION AND MOST POSSIBLE CANDIDATEGENES TO SC20 RESISTANCE | 第121-122页 |
| REFERENCES | 第122-148页 |
| APPENDICES | 第148-180页 |
| ACKNOWLEDGEMENT | 第180-182页 |
| DEDICATION | 第182-184页 |
| BIOGRAPHICAL DATA | 第184-185页 |
