| ABSTRACT | 第5-6页 |
| 摘要 | 第7-8页 |
| 附图 | 第8-11页 |
| CHAPTER ONE: INTRODUCTION | 第11-23页 |
| 1.1 General view | 第11-12页 |
| 1.2 Necessity of study and destructive impacts of biological pesticides | 第12-13页 |
| 1.3 Natural occurrence of the entomopathogenic fungi species in nature | 第13-17页 |
| 1.4 Cryptic species in fungal entomopathogens and the use of molecular delimitation forecological studies | 第17-19页 |
| 1.5 Are fungi relatively free from dispersal barriers? | 第19-20页 |
| 1.6 The applicability of the beneficial symbioant to fungal entomopathogens | 第20-21页 |
| 1.7 Entomopathogenic fungi as a biological control agent | 第21页 |
| 1.8 Thesis scope | 第21-23页 |
| CHAPTER TWO: GENERAL METHODS | 第23-36页 |
| 2.1 Study site and soil sampling | 第23-25页 |
| 2.2 Isolation of insect pathogenic fungi (Metarhizium and Beauveria) from FNNR soilsamples by an in vitro semi-differential medium method | 第25-26页 |
| 2.3 Fungal isolation from Sichuan province soil samples with insect susceptible bait method | 第26-27页 |
| 2.4 Morphology of 20 Metarhizium isolates from FNNR | 第27页 |
| 2.5 Genomic DNA extraction, PCR amplification and sequencing: | 第27-30页 |
| 2.6 Phylogenetic analysis: | 第30-31页 |
| 2.7 Bioassay experiment: | 第31-34页 |
| 2.7.1 Experimental insects | 第31-32页 |
| 2.7.2 Preparation of conidia | 第32-34页 |
| 2.8 Cold activity on conidial germination | 第34页 |
| 2.9 Deposit of fungal strain in public service collection | 第34页 |
| 2.10 Statistical analysis | 第34-35页 |
| 2.11 Mantel test and species richness | 第35-36页 |
| CHAPTER THREE: RESULTS | 第36-66页 |
| 3.1 RESULTS OF EFs FROM FNNR | 第36-51页 |
| 3.1.1 Metarhizium occurrence and DNA analysis | 第36-41页 |
| 3.1.2 Morphology of isolates and culture studies | 第41-43页 |
| 3.1.3 Growth assessment and in vitro conidiation study | 第43-44页 |
| 3.1.4 Virulence | 第44-46页 |
| 3.1.5 Pathogenicity against L. dispar | 第46页 |
| 3.1.6 Cold activity | 第46-51页 |
| 3.2 Results from Sichuan Province | 第51-66页 |
| 3.2.1 Occurrence and distribution of the entomopathogenic fungi | 第51-56页 |
| 3.2.2 Phylogenetic analysis of fungal isolates | 第56-66页 |
| CAHPTER FOUR: DISCUSSION AND CONCLUSION | 第66-79页 |
| 4.1 Discussion | 第66-77页 |
| 4.2 Conclusion | 第77-79页 |
| CHAPTER FIVE: SUPPLEMENTARY DATA | 第79-102页 |
| References | 第102-114页 |
| LIST OF FIGURES | 第114-116页 |
| LIST OF TABLES | 第116-117页 |
| List of Abbreviations | 第117-118页 |
| Aknowledgement | 第118-119页 |
| Personal introduction | 第119页 |
| List of Publications | 第119页 |
