| 摘要 | 第3-4页 |
| ABSTRACT | 第4-5页 |
| CHAPTER 1 INTRODUCTION | 第18-38页 |
| 1.1 Research background | 第18-25页 |
| 1.1.1 Air pollution | 第18页 |
| 1.1.2 Volatile Organic Compounds (VOCs) | 第18-20页 |
| 1.1.3 Biofilter, an efficient biological system to control VOCs | 第20-22页 |
| 1.1.4 Bed clogging and ozone injection | 第22-25页 |
| 1.2 Literature review | 第25-35页 |
| 1.2.1 Effect of ozone on the long-term performance of biofilter | 第25-28页 |
| 1.2.2 Effect of ozone on the biomass content in biofilter | 第28-29页 |
| 1.2.3 Effect of ozone on the microbial community structure inside biofilter | 第29-31页 |
| 1.2.4 Effect of ozone on the microbial activity in biofilter | 第31-32页 |
| 1.2.5 Ozone-tolerant microorganisms | 第32-35页 |
| 1.3 Research opportunities | 第35页 |
| 1.4 Research objectives and contents | 第35-36页 |
| 1.5 Road map | 第36-38页 |
| CHAPTER 2 RESEARCH METHODOLOGY | 第38-50页 |
| 2.1 Experiment configuration | 第38页 |
| 2.2 Inoculation and operation conditions of biofilters | 第38-40页 |
| 2.3 Analysis of toluene removal performance | 第40-41页 |
| 2.3.1 Toluene removal efficiency | 第40-41页 |
| 2.3.2 The pressure drop | 第41页 |
| 2.4 Analysis of biomass and carbon balance | 第41-43页 |
| 2.4.1 Biofilter biomass detection | 第41页 |
| 2.4.2 CO_2 detection | 第41-42页 |
| 2.4.3 Detection of total carbon (TC) in leachate | 第42页 |
| 2.4.4 The carbon balance analysis | 第42-43页 |
| 2.5 Biofilm charavteristics | 第43-45页 |
| 2.5.1 Long-term exposure of biofilm in the biofilter to ozone | 第43-45页 |
| 2.5.2 Short term exposure of biofilm to ozone | 第45页 |
| 2.6 Analysis of microbial community structure and functions | 第45-50页 |
| 2.6.1 Total plate count | 第46页 |
| 2.6.2 DNA extraction and PMA treatment procedure | 第46-47页 |
| 2.6.3 The microbial viability detected by PMA-q PCR | 第47-48页 |
| 2.6.4 Microbial community structure by Miseq high throughput sequencing | 第48-49页 |
| 2.6.5 Metabolic characteristics by Biolog test | 第49-50页 |
| CHAPTER 3 EFFECT OF OZONE ON LONG-TERM PERFORMANCE OF BIOFILTERS | 第50-58页 |
| 3.1 The operating conditions of the biofilter | 第50-52页 |
| 3.1.1 Temperature and humidity | 第50-51页 |
| 3.1.2 pH | 第51-52页 |
| 3.2 Toluene inlet loading of biofilters | 第52-53页 |
| 3.3 Effect of ozone on long-term toluene removal efficiency | 第53-55页 |
| 3.4 Effect of ozone on pressure drop of long-term biofilter | 第55-57页 |
| 3.5 Chapter summary | 第57-58页 |
| CHAPTER 4 EFFECT OF OZONE ON BIOMASS ACCUMULATION AND CARBON BALANCE | 第58-65页 |
| 4.1 Effect of ozone on the biomass growth | 第58-59页 |
| 4.2 Effect of ozone on the CO_2 mineralization | 第59-60页 |
| 4.3 Effect of ozone on the total carbon in the leachate | 第60-61页 |
| 4.4 Effect of ozone on carbon balance in long-term biofilter | 第61-64页 |
| 4.5 Chapter summary | 第64-65页 |
| CHAPTER 5 MODELLING BIOMASS ACCUMULATION WITH AND WITHOUT OZONE INJECTION | 第65-72页 |
| 5.1 Model development | 第65-66页 |
| 5.2 Model solution and calculation | 第66页 |
| 5.3 Parameter estimation | 第66-68页 |
| 5.3.1 X_(at)_0 and X_(it)_0 | 第66-67页 |
| 5.3.2 β, Y, and b | 第67页 |
| 5.3.3 –r | 第67页 |
| 5.3.4 0_ι | 第67-68页 |
| 5.3.5 k_(0_3,bio) | 第68页 |
| 5.4 Model verification | 第68-71页 |
| 5.5 Chapter summary | 第71-72页 |
| CHAPTER 6 EFFECT OF OZONE ON THE CHARACTERISTICS OF BIOFILM BY CLSM | 第72-85页 |
| 6.1 Long time exposure of biofilm in the biofilter to ozone | 第72-82页 |
| 6.1.1 Characteristics of biofilm grown on input glass slide | 第72-75页 |
| 6.1.2 Characteristics of biofilm grown on perlite packing media | 第75-76页 |
| 6.1.3 Transformation of cell viability and Gram category predominance | 第76-77页 |
| 6.1.4 Transformation of biofilm thickness | 第77-78页 |
| 6.1.5 Quantitative analysis of cell viability transformation | 第78-79页 |
| 6.1.6 Quantitative analysis of Gram-typed cell transformation | 第79-82页 |
| 6.2 Short time exposure of biofilm to ozone | 第82-84页 |
| 6.2.1 Effects of 200 mg/m~3 on toluene removal biofilm | 第82-83页 |
| 6.2.2 Effects of 400 and 800 mg/m~3 on toluene removal biofilm | 第83-84页 |
| 6.3 Chapter summary | 第84-85页 |
| CHAPTER 7 EFFECT OF OZONE ON MICROBIAL COMMUNITY STRUCTURE AND FUNCTIONS | 第85-100页 |
| 7.1 Effect of ozone on microbial cell viability | 第85-89页 |
| 7.1.1 The analysis by total plate count approach | 第85-86页 |
| 7.1.2 The analysis by quantitative polymerase chain reaction | 第86-89页 |
| 7.2 Effect of ozone on the microbial community structure | 第89-95页 |
| 7.3 Effect of ozone on metabolic characteristics | 第95-98页 |
| 7.4 Chapter summary | 第98-100页 |
| CHAPTER 8 CONCLUSIONS AND SUGGESTIONS | 第100-103页 |
| 8.1 Conclusions | 第100-102页 |
| 8.2 Suggestions | 第102-103页 |
| REFERENCES | 第103-110页 |
| ACKNOWLEDGEMENTS | 第110-112页 |
| RESUME | 第112-113页 |
| PUBLISHED PAPERS | 第113页 |
