| ABSTRACT | 第8-10页 |
| 摘要 | 第11-13页 |
| List of abbreviation | 第13-15页 |
| CHAPTER 1 Literature review and study plan | 第15-33页 |
| 1. Definition of aquaculture | 第15-16页 |
| 2. Crustaceans | 第16-18页 |
| 3. Macrobrachium nipponense distribution and aquaculture | 第18-19页 |
| 4. Overview of aquatic animals Genetics | 第19-20页 |
| 5. Sex management and breeding | 第20-21页 |
| 6. Future evolution | 第21页 |
| 7. Needs for Sex Control | 第21-22页 |
| 8. Sex determination and sex differentiation in fish | 第22-23页 |
| 9. Approaches of Sex Manipulation | 第23-24页 |
| 10. Gene research in crustaceans | 第24-25页 |
| 11. Genetics and genes research in Macrobrachium nipponense | 第25-30页 |
| 12. Androgenic gland | 第30-31页 |
| 13. Justification of the study | 第31页 |
| 14. Aim of the study | 第31页 |
| 15. Hypothesis of the study | 第31-32页 |
| 16. Content of the study, gene selection and study schematic design | 第32-33页 |
| CHAPTER 2 Molecular cloning and expression analysis of Fem1b from Oriental River prawnMacrobrachium nipponense | 第33-51页 |
| 1 Introduction | 第33-34页 |
| 2 Materials and methods | 第34-39页 |
| 2.1 Prawn and tissue preparation | 第34页 |
| 2.2 RNA isolation and reverse transcription | 第34-35页 |
| 2.3 Rapid amplification of cDNA ends (RACE) | 第35-36页 |
| 2.4 Nucleotide sequence and bioinformatics analyses | 第36-37页 |
| 2.5 Real-time quantitative PCR analysis of Femlb | 第37-38页 |
| 2.6 Statistical analysis | 第38-39页 |
| 3 Results | 第39-47页 |
| 3.1 Sequence analysis of MnFem1b | 第39页 |
| 3.2 Homology and phylogenetic analysis of MnFem1b | 第39-45页 |
| 3.3 Tissue distribution of MnFem1b mRNA | 第45-46页 |
| 3.4 Expression analysis of MnFem1b mRNA during embryo,larvae,andpost-larval stages | 第46-47页 |
| 4 Discussion | 第47-49页 |
| 5 Conclusion | 第49-51页 |
| CHAPTER 3 Molecular cloning and expression patter of Nitric Oxide Synthase (NOS) in oriental riverprawn, Macrobrachium nipponense | 第51-69页 |
| 1 Introduction | 第51-52页 |
| 2 Materials and Methods | 第52-57页 |
| 2.1 Experimental Prawn and tissue collection | 第52-53页 |
| 2.2 RNA extraction and cDNA synthesis | 第53页 |
| 2.3 5'-and 3'-random amplification of cDNA ends (RACE) of NOS gene | 第53-55页 |
| 2.4 Analyses of the nucleotide and deduced amino acid sequence | 第55页 |
| 2.5 Expression of MnNOS in different adult tissues and developmental stages | 第55-56页 |
| 2.6 Statistical analysis | 第56-57页 |
| 3 Results | 第57-65页 |
| 3.1 Characterization of the full-length of MnNOS | 第57-61页 |
| 3.2 Phylogenetic analysis of MnNOS | 第61-62页 |
| 3.3 Expression of MnNOS mRNA in tissues | 第62-63页 |
| 3.4 Temporal expression of MnNOS mRNA during the different developmentalstages of prawn larvae, embryo and post larvae | 第63-65页 |
| 4 Discussion | 第65-68页 |
| 5 Conclusion | 第68-69页 |
| CHAPTER 4 Molecular Cloning and Expression Profile of Glycogen Debranching Enzyme-like Geneduring Developmental Stages of Macrobrachium nipponense(de Haan 1849) | 第69-91页 |
| 1 Introduction | 第69-70页 |
| 2 Material and methods | 第70-77页 |
| 2.1 Prawn and tissue preparation | 第70-71页 |
| 2.2 RNA isolation and reverse transcription | 第71页 |
| 2.3 Rapid amplification of cDNA ends of MnAGL (RACE) | 第71-72页 |
| 2.4 Nucleotide sequence and bioinformatics analyses | 第72-75页 |
| 2.5 Real-time quantitative PCR analysis of MnAGL | 第75页 |
| 2.6 Statistical analysis | 第75-77页 |
| 3 Result | 第77-87页 |
| 3.1 Structural analysis of a full-length cDNA of MnAGL | 第77页 |
| 3.2 Homology and phylogenetic analysis of AGL | 第77-81页 |
| 3.3 Phylogenetic analysis of MnAGL | 第81-84页 |
| 3.4 Tissue distribution of the MnAGL gene transcript | 第84-85页 |
| 3.5 Temporal expression of MnAGL mRNA during the early embryo,larvae andpost-larvae stages | 第85-87页 |
| 4 Discussion | 第87-89页 |
| 5 Conclusion | 第89-91页 |
| CHAPTER 5 Molecular cloning of two Serine protease inhibitor genes and expression analysis indifferent tissues in oriental river prawn,Macrobrachium nipponense | 第91-105页 |
| 1 Introduction | 第91-93页 |
| 2 Materials and methods | 第93-94页 |
| 2.1 Shrimp | 第93页 |
| 2.2 Total RNA extraction and first-strand cDNA synthesis | 第93页 |
| 2.3 Rapid amplification of cDNA ends (RACE) | 第93-94页 |
| 2.4 Tissue distribution analysis | 第94页 |
| 2.5 Nuleotide sequence and Bioinformatics analysis | 第94页 |
| 3 Results | 第94-103页 |
| 3.1 Sequence analysis of MnSERPIN1 and MnSERPIN2 | 第94-96页 |
| 3.2 Bioinformatics analysis | 第96-98页 |
| 3.3 Homologous and phylogenetic analysis of MnSERPIN1 and MnSERPIN2 | 第98-101页 |
| 3.4 Tissue expression of MnSERPIN1 and MnSERPIN2 | 第101-103页 |
| 4 Discussion | 第103-104页 |
| 5 Conclusion | 第104-105页 |
| Conclusion of the whole study | 第105-107页 |
| References | 第107-125页 |
| Acknowledgements | 第125-127页 |
| Publications | 第127页 |
| Academic award | 第127页 |
| Academic seminars attended | 第127-128页 |
