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外源NO对镉胁迫下紫花苜蓿种子萌发及生长的影响

ABSTRACT第4-9页
摘要第10-16页
1 Introduction第16-23页
    1.1 Heavy metal stress in plant第16-18页
    1.2 Effects of NO on seed germination and seedling growth under heavy metals stress第18-20页
        1.2.1 Effects on germination and seedling growth第18-19页
        1.2.2 Effects on ROS generation第19页
        1.2.3 Effects on anti-oxidant enzyme activities第19-20页
        1.2.4 Effects on storage substances in seeds第20页
        1.2.5 Effects on Cadmium accumulation in plant第20页
    1.3 Alfalfa第20-21页
    1.4 Purpose and significance of the research第21-22页
    1.5 Research outline第22-23页
2 Materials and methods第23-30页
    2.1 Seeds treatment and germination conditions第23页
    2.2 Assay method第23-29页
        2.2.1 Germination parameters第23-24页
        2.2.2 Determination of proline content第24页
        2.2.3 Relative electric conductivity第24页
        2.2.4 Fresh and dry weight measurement第24页
        2.2.5 Determination of ROS metabolism第24-27页
        2.2.6 Determination of storage substances第27-29页
        2.2.7 Determination of Cadmium content第29页
    2.3 Data analysis第29-30页
3 Results第30-42页
    3.1 Effect of exogenous NO on germination and seedling growth第30-31页
    3.2 Effect of exogenous NO on reactive oxygen species第31-38页
        3.2.1 Effect of exogenous NO on MDA, H2O2, O2- and REC levels第31-35页
        3.2.2 Effect Of exogenous NO on antioxidant enzymes activity and Pro content第35-38页
    3.3 Effect of exogenous NO on storage substances第38-41页
    3.4 Effect of exogenous NO on Cadmium accumulation第41-42页
4 Discussion第42-48页
    4.1 Exogenous NO alleviates inhibitory effects of Cd on germination and growth第42-43页
    4.2 Exogenous NO regulates ROS metabolism第43-46页
    4.3 Exogenous NO regulates storage substances第46-47页
    4.4 Exogenous NO reduces Cadmium accumulation第47-48页
Conclusion and future perspectives第48-49页
Acknowledgement第49-50页
References第50-55页
Published papers第55页

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