| 中文摘要 | 第1-6页 |
| Abstract | 第6-11页 |
| ABBREVIATIONS | 第11-13页 |
| Chapter 1 REVIEW OF THE LITERATURE | 第13-39页 |
| ·MASTITIS | 第13-15页 |
| ·Description | 第13-14页 |
| ·The Mammary Gland | 第14页 |
| ·Mammary Epithelial Cells(MEC) | 第14-15页 |
| ·Mastitis Pathogens | 第15-22页 |
| ·Staphylococcus aureus | 第16-19页 |
| ·Trueperella (Arcanobacterium) pyogenes | 第19-22页 |
| ·Bacterial survival strategies | 第22-38页 |
| ·Antibiotic resistance | 第23-28页 |
| ·Forming biofilm (slime) | 第28-30页 |
| ·Forming small colony variants (SCVs) | 第30-37页 |
| ·Searching for novel antibiotics targets | 第37-38页 |
| ·Objective and meaning of the studies described in this Ihesis | 第38-39页 |
| Chapter 2:Properties of Staphylococcus aureus Isolated from Mastitis at twoDairy Herds in China | 第39-62页 |
| ·Molecular methods for PCR-mediated identification | 第40页 |
| ·16S rRNA gene | 第40页 |
| ·Random amplified polymorphic DNA (RAPD) | 第40页 |
| ·The role of bacterial virulence factors in Staphylococcus aureus intramammaryinfection | 第40-42页 |
| ·Control and expression of virulence factors | 第41页 |
| ·Virulence factors involved in establishing an infection | 第41-42页 |
| ·Virulence factors involved in maintenance of infection | 第42页 |
| ·Staphylococcal biofilm formation | 第42-43页 |
| ·Molecules responsible for exopolysaccharide production | 第43页 |
| ·PIA production and the intracellular adhesion locus | 第43页 |
| ·Antimicrobial resistance of bovine Staphylococcus aureus | 第43-44页 |
| ·Materials and methods | 第44-53页 |
| ·Origin of S. aureus isolates | 第44页 |
| ·Study animals and herds | 第44页 |
| ·Clinical examination of the cows and mastitis follow-up | 第44-45页 |
| ·Sample collection | 第45页 |
| ·Bacterial isolation and conventional identification | 第45-46页 |
| ·Biofilm formation | 第46-47页 |
| ·DNA extraction and preparation | 第47-50页 |
| ·Detection of genes encoding virulence and adhesion factors using PCR | 第50页 |
| ·Detection of ica locus by PCR | 第50-52页 |
| ·Antimicrobial susceptibility testing | 第52-53页 |
| ·Results | 第53-58页 |
| ·Bacterial isolation and conventional identification | 第53-54页 |
| ·Random amplified polymorphic DNA (RAPD) | 第54-55页 |
| ·Biofilm formation | 第55-56页 |
| ·S.aureus virulence gene profiles | 第56页 |
| ·Detection of ica locus by PCR | 第56-57页 |
| ·Antimicrobial resistance of S.aureus | 第57-58页 |
| ·Discussion | 第58-61页 |
| ·Conclusion | 第61-62页 |
| Chapter3:Characteristics of Trueperella pyogenes isolated from bovinemastitis | 第62-83页 |
| ·Putative virulence factors of A.(T.) pyogenes | 第63-64页 |
| ·Pyolysin | 第63页 |
| ·Collagen-binding proteins | 第63页 |
| ·Exoenzymes | 第63-64页 |
| ·Fimbriae | 第64页 |
| ·Hemolytic properties and CAMP-like hemolytic reactions | 第64页 |
| ·Materials and methods | 第64-71页 |
| ·Isolation and identification of T. pyogenes from milk samples | 第64-65页 |
| ·Identification and further characterization of the bacteria by conventional methods | 第65页 |
| ·Biochemical tests | 第65-67页 |
| ·Growth curve | 第67页 |
| ·Growth on Loeffler medium | 第67-68页 |
| ·Identification and molecular characterization of the bacteria by PCR | 第68页 |
| ·Detection of potential virulence factor and antibiotic resistance genes | 第68-69页 |
| ·Biofilm production | 第69页 |
| ·Antibiotic resistance | 第69页 |
| ·Intracellular assessment of T. pyogenes | 第69-70页 |
| ·Preservation of the bacteria | 第70-71页 |
| ·Results | 第71-80页 |
| ·Isolation and identification of T. pyogenes from milk | 第71页 |
| ·Biochemical properties and CAMP-like hemolytic reactions | 第71-72页 |
| ·Growth curve | 第72-73页 |
| ·Analysis of DNA amplification products obtained by RAPD | 第73-74页 |
| ·Biofilm production | 第74页 |
| ·Antibiotic resistance | 第74-76页 |
| ·Intracellular assessment of T. pyogenes | 第76-77页 |
| ·Genes encoding potential virulence factors | 第77-80页 |
| ·Discussion | 第80-82页 |
| ·Conclusion | 第82-83页 |
| CHAPTER 4:Characteristics of Staphylococcus aureus and Trueperellapyogenes-Small Colony Variants and their Parent Strains Isolated fromChronic Mastitis | 第83-114页 |
| ·Characteristics of Staphylococcus aureus Small Colony Variant and Its Parent Strain Isolated from Chronic Mastitis | 第84-101页 |
| ·Project aims | 第84-86页 |
| ·Materials and methods | 第86-91页 |
| ·Results | 第91-97页 |
| ·Discussion | 第97-99页 |
| ·Conclusion | 第99-101页 |
| ·Isolation and characterization of novel Trueperella pyogenes small colony variants from bovine mastitis cases | 第101-114页 |
| ·Project aims | 第101页 |
| ·Materials and methods | 第101-104页 |
| ·Results | 第104-111页 |
| ·Discussion | 第111-113页 |
| ·Conclusion | 第113-114页 |
| Chapter 5:Conclusions | 第114-115页 |
| References | 第115-137页 |
| Acknowledgement | 第137-139页 |
| 个人简历 | 第139-140页 |
