| Abstract | 第1-7页 |
| 中文摘要 | 第7-9页 |
| Abbreviations | 第9-11页 |
| Chapter Ⅰ Review | 第11-51页 |
| ·History and development of induced pluripotent stem cells | 第12-29页 |
| The concepts and importance of pluripotent stem cells | 第12-17页 |
| Development of artificial pluripotent stem cells | 第17-21页 |
| Problems in generation of clinical applicable induced pluripotent stemcells | 第21-29页 |
| ·Reprogramming factors and molecular pathways in induction of iPS cells | 第29-36页 |
| Traditional reprogramming genes in induction of iPS cells | 第29-33页 |
| Other reprogramming factors involved in the induction of iPS cells | 第33-34页 |
| Molecular mechanisms underlying the induction of iPS cells | 第34-36页 |
| Reference | 第36-51页 |
| Chapter Ⅱ:Stable Intermediate Phase in ReprogrammingProcess and Two-Phase Induction of iPS Cells | 第51-78页 |
| Introduction | 第52-54页 |
| Methods and Materials | 第54-61页 |
| ·An possible stable intermediate phase from an abnormal iPS cell line | 第61-67页 |
| Comparison of iPS cell lines and ES cell lines | 第62-63页 |
| A possible intermediate phase from subclones of TT-025 | 第63-65页 |
| Reprogram the possible intermediate phase to pluripotent phase | 第65-67页 |
| ·Confirmation of the intermediate phase inreprogramming process and two-phase induction ofiPS cells | 第67-72页 |
| Induction of intermediate phase cells directly from somatic cells | 第68-70页 |
| Two-phase induction of iPS cells | 第70-72页 |
| Discussion | 第72-73页 |
| Contributions | 第73-74页 |
| References | 第74-78页 |
| Chapter Ⅲ:Analysis of Molecular Pathways UnderlyingTwo-Phase Induction of iPS Cells | 第78-107页 |
| Introduction | 第79-81页 |
| Methods and Mate rials | 第81-85页 |
| ·Global gene expression analysis of two-phase induction of iPS cells | 第85-92页 |
| Global microarray analysis of Sox2 downstream genes | 第85-88页 |
| Confirmation of Sox2 downstream gene change in reprogramming process | 第88-92页 |
| ·Molecular mechanisms underlying two-phase induction of iPS cells | 第92-98页 |
| Temporal expression patterns of Sox2-regulated genes | 第92-93页 |
| DNA methylation analysis | 第93-95页 |
| One critical Sox2 signaling pathway for late-phase iPS cellreprogramming | 第95-98页 |
| Discussion | 第98-102页 |
| Contribution | 第102页 |
| References | 第102-107页 |
| Acknowledgments | 第107-109页 |
| Publication | 第109-111页 |
