| CHAPTER I Introduction to the model organism Caenorhabditis elegans | 第1-18页 |
| Origins of the model | 第13页 |
| Powerful biology model | 第13-14页 |
| Genome | 第14-15页 |
| Genetics | 第15-16页 |
| Neurobiology | 第16-18页 |
| CHAPTER IICaenorhabditis elegans sister chromatid cohesion proteinsEVL-14/PDS-5 and SCC-3 are required for both meiosis and mitosis | 第18-53页 |
| Introduction | 第19-23页 |
| ResultsMutations in evl-14 and scc-3 disrupted the development ofthe vulva and gonad | 第23-29页 |
| evl-14 and scc-3 encode the likely homologs of the yeast Pds5 and Scc3respectively | 第29-38页 |
| Loss of evl-14 and scc-3 gene functions disrupt sister chromatid cohesionduring meiosis | 第38-45页 |
| The localization of REC-8 to chromosomes is completely disruptedin scc-3 mutants | 第45-48页 |
| scc-3 RNAi results in embryonic lethalityand evl-14/pds-5 RNAi causes a range of phenotypes | 第48-52页 |
| evl-14/pds-5 is expressed in embryo and postembryonic dividing cells | 第52-53页 |
| DiscussionPvl Ste mutants primarily represent genes involved in cell divisionand cell cycle regulation | 第53-60页 |
| EVL-14/PDS-5 and SCC-3 function in both mitosis and meiosis | 第53-54页 |
| SCC-3 is essential for sister chromatid cohesion | 第54-57页 |
| EVL-14/PDS-5 is important for maintaining sister chromatid cohesionin late prophase | 第57-58页 |
| Model of cohesin | 第58-60页 |
| Materials and methods | 第60-65页 |
| C.e;egams Strains | 第60页 |
| Genetic mapping and molecular cloning | 第60-61页 |
| Double-stranded RNAi | 第61-62页 |
| Gonad preparation and immunostaining | 第62页 |
| DAPI staining and FISH | 第62-63页 |
| Microscopy | 第63-64页 |
| GFP reporter construction | 第64-65页 |
| ACKNOWLEDGMENTS | 第65-66页 |
| REFERENCES | 第66-70页 |
| PUBLICATIONS | 第70-71页 |
