| Abbreviations | 第7-9页 |
| 中文摘要 | 第9-12页 |
| ABSTRACT | 第12-15页 |
| 1 Introduction | 第16-44页 |
| 1.1 Physiological features in seed germination | 第17-18页 |
| 1.2 Transcriptomic approach to address seed germination | 第18-28页 |
| 1.2.1 Energy production for germination | 第19-22页 |
| 1.2.2 Genes involve in starch and sucrose metabolism | 第22-26页 |
| 1.2.3 Genes involve in protein mobilization | 第26页 |
| 1.2.4 Activation of developmental genes | 第26-27页 |
| 1.2.5 Defense genes activation | 第27页 |
| 1.2.6 Phytohormones in seed germination | 第27-28页 |
| 1.3 Proteomic approach to address seed germination | 第28-34页 |
| 1.3.1 Proteins involved in regulating germination | 第28-30页 |
| 1.3.2 Progress in proteomics related to seed germination | 第30-32页 |
| 1.3.3 Proteins involved in seed germination | 第32-34页 |
| 1.4 Transcriptome and proteomics interaction | 第34-42页 |
| 1.4.1 Transcription and translation activation | 第36-38页 |
| 1.4.2 Translation role in seed germination | 第38-39页 |
| 1.4.3 Comparison of the seed transcriptome and proteome | 第39-41页 |
| 1.4.4 Post-translational control of seed germination | 第41-42页 |
| 1.5 Objectives and technical route | 第42-44页 |
| 1.5.1 Objectives | 第42-43页 |
| 1.5.2 Technical route | 第43-44页 |
| 2 Transcriptome analysis in Cyclobalnopsis gilva seed germination | 第44-66页 |
| 2.1 Materials and methods | 第45-49页 |
| 2.1.1 Plant materials | 第45-46页 |
| 2.1.2 RNA extraction and construction of cDNA libraries for transcriptomesequencing | 第46页 |
| 2.1.3 Illumina sequencing, assembly and annotation | 第46-47页 |
| 2.1.4 Analysis of quantitative real-time PCR (qRT-PCR) | 第47-48页 |
| 2.1.5 Analysis of physiological parameters | 第48页 |
| 2.1.6 Statistical analysis | 第48-49页 |
| 2.2 Results | 第49-61页 |
| 2.2.1 Sequence analysis and assembly from C. gilva seeds | 第49页 |
| 2.2.2 Analysis of differentially expressed unigenes (DEGs) by RNA-seq | 第49-54页 |
| 2.2.3 DEGs validation during seed germination | 第54-59页 |
| 2.2.4 Physiological profiles during seed germination | 第59-61页 |
| 2.3 Discussion | 第61-66页 |
| 2.3.1 Transcriptional responses in the glycolysis and TCA cycle pathways | 第61-62页 |
| 2.3.2 Transcriptional responses in starch and sucrose metabolism | 第62-66页 |
| 3 Proteomic study on Cyclobalnopsis gilva seed germination | 第66-97页 |
| 3.1 Proteomic 2-DE approach to address low germination rate in C. gilva seeds | 第66-78页 |
| 3.1.1 Materials and methods | 第67-70页 |
| 3.1.2 Results | 第70-75页 |
| 3.1.3 Discussion | 第75-78页 |
| 3.2 Label-Free quantitative proteomics analysis in C. gilva seed germination | 第78-97页 |
| 3.2.1 Materials and methods | 第79-83页 |
| 3.2.2 Results | 第83-94页 |
| 3.2.3 Discussion | 第94-97页 |
| 4 Conclusion | 第97-99页 |
| Reference | 第99-115页 |
| Publication | 第115-116页 |
| Acknowledgement | 第116-117页 |
