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白菜RING基因家族的全基因组鉴定与表达分析

摘要第7-9页
Abstract第9页
Chapter 1 Introduction第11-17页
    1.1 Ubiquitin System第11-13页
        1.1.1 Ubiquitin activating enzyme E1第11-12页
        1.1.2 Ubiquitin conjugating enzymes E2s第12页
        1.1.3 Ubiquitin E3 ligases-Enzyme第12-13页
    1.2 RING finger protein第13-14页
        1.2.1 Amino acid composition第14页
    1.3 Biological processes第14-15页
    1.4 Brassicaceae or Cruciferae第15-17页
Chapter 2 Materials and methods第17-21页
    2.1 Identification of RING finger proteins in B. rapa第17页
    2.2 Multiple sequence alignment and phylogenetic analysis第17-18页
    2.3 Identification of additional domains in RING domain containing B. rapa proteins第18页
    2.4 Syntenic relationships between the RING finger protein genes of B. rapa and A. thaliana第18页
    2.5 Chromosome location of the B. rapa RING finger protein genes第18-19页
    2.6 Expression analysis of RING domain-containing genes in different tissues of B. rapa.第19页
    2.7 Plant materials第19页
    2.8 Semi-Quantitative RT-PCR analysis第19-20页
    2.9 Plant Materials and Stress Treatments第20页
    2.10 RNA isolation and quantitative real-time PCR (qRT-PCR) analysis第20-21页
Chapter 3 Results第21-41页
    3.1 Genome-wide identification of RING finger proteins in B. rapa第21-22页
    3.2 Spacing conservation between metal ligands in B. rapa RING domains第22-26页
    3.3 Conservation of other residues in B. rapa RING domains第26-27页
    3.4 Phylogenetic analysis of the B. rapa RING domains第27页
    3.5 Additional domains in the RING protein genes第27-28页
    3.6 Chromosomal location of RING finger genes on B. rapa genome第28-31页
    3.7 Syntenic relationships between RING finger genes of B. rapa and A. thaliana第31页
    3.8 Expression analysis of B. rapa RING finger genes in different tissues第31-35页
    3.9 Semi-Quantitative RT-PCR analysis第35-37页
    3.10 Expression Analysis of B. rapa RING finger Genes under Salt Stress第37-39页
    3.11 Expression Analysis of B. rapa RING finger Genes under Drought Stress第39-41页
Discussion第41-43页
Conclusion第43-44页
References第44-51页
Published Paper第51-52页
Acknowledgement第52-53页
Appendix Ⅰ第53-109页
Appendix Ⅱ第109-148页

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