| 中文摘要 | 第1-7页 |
| Abstract | 第7-9页 |
| LIST OF ABBREVIATIONS | 第9-11页 |
| TABLE OF CONTENTS | 第11-14页 |
| Chapter 1. BACKGROUND AND LITERATURE REVIEW | 第14-35页 |
| ·About health benefits | 第15-17页 |
| ·Biosynthetic pathways involved in soy isoflavones | 第17-20页 |
| ·MYB Transcription factor and relationship with soy isoflavone | 第20-25页 |
| ·Types and distribution of soy isoflavones | 第22页 |
| ·Key enzymes involved in the biosynthesis of isoflavones | 第22-24页 |
| ·Isoflavones physiological functions | 第24-25页 |
| ·Overview of GM crops and transgenic soybeans | 第25-33页 |
| ·The purpose and significance of the study | 第33-35页 |
| Chapter 2. FACTORS RESPONSIBLE FOR THE EFFICIENCY OF SOYBEAN USING AGROBACTERIUM- MEDIATED TRANSFORMATION METHOD | 第35-46页 |
| ·Materials | 第35页 |
| ·Plant materials | 第35页 |
| ·Reagents, experimental equipments and instruments | 第35页 |
| ·Methods | 第35-40页 |
| ·Effect of Optical Density (OD) services Agrobacterium on the gene transformation | 第37页 |
| ·Effect of time of infection by means of vacuum on the gene transformation | 第37-38页 |
| ·Effect of concentrated Acetosyringon (AS) on the gene transformation | 第38页 |
| ·Effect of L-Cysteine concentration on the gene transformation | 第38页 |
| ·Temporary expression of GUS | 第38-40页 |
| ·Results and Discussion | 第40-45页 |
| ·Effect of Agrobacterium tumefaciens suspention optical density (OD) on the gene transformation | 第40-41页 |
| ·Effect of time of effection by means of vacuum on the gene transformation | 第41-42页 |
| ·Effect of AS concentration on a temporary rate of gene expression associated GUS MYB12A. gene | 第42-44页 |
| ·Effect of L-Cys concentration on transformation efficiency | 第44-45页 |
| ·Conclusion | 第45-46页 |
| Chapter 3. THE RESEARCH ON GmMYB12A, AND GmMYB12B2 GENE TO TRANSFER EFFICIENCIES OF SOME SOYBEAN GENOTYPE BY HALF-SEED EXPLANT METHOD | 第46-87页 |
| ·Materials | 第46-48页 |
| ·Plant materials | 第46页 |
| ·Genetic material | 第46-48页 |
| ·Reagents, experimental equipments and instruments | 第48页 |
| ·Methods | 第48-69页 |
| ·Cloning and sequence analysis in transcriptional factor of soybean MYB12A and MYB12B2 gene method | 第49-54页 |
| ·Agrobacterium tumefaciens preparation | 第54-55页 |
| ·Media composition and preparation of cultures | 第55页 |
| ·Molecular analysis | 第55-69页 |
| ·Results and Discussion | 第69-86页 |
| ·Infection and co-cultivation | 第69-71页 |
| ·Confirmation of Transformation on Shoot induction medium | 第71-73页 |
| ·Shoot elongation | 第73-76页 |
| ·Root induction of the putative transformed shoots | 第76-78页 |
| ·Confirmation of transgenic soybean | 第78-86页 |
| ·Conclusion | 第86-87页 |
| Chapter 4. OPTIMIZATION OF MULTIPLE SHOOT INDUCTION OF JILIN SMALL 7GRANULE SOY | 第87-102页 |
| ·Materials | 第87页 |
| ·Plant materials | 第87页 |
| ·Reagents, experimental equipments and instruments | 第87页 |
| ·Methods | 第87-89页 |
| ·Preparation of explants | 第87-88页 |
| ·Media composition and preparation of cultures | 第88-89页 |
| ·Statistical analysis | 第89页 |
| ·Results and Discussion | 第89-101页 |
| ·Germination rate of different sterilization times | 第89-90页 |
| ·Effect BAP on in vitro multiple shoot formation | 第90-93页 |
| ·Effects of different concentrations of Tria on TDZ (1 μM) on multiple shoot induction rate | 第93-95页 |
| ·Effect of IBA and IAA on in vitro rooting medium | 第95-98页 |
| ·Hardening of plantlets | 第98-101页 |
| ·Conclusions | 第101-102页 |
| Chapter 5. CONCLUSION | 第102-104页 |
| REFERENCES | 第104-115页 |
| APPENDIX | 第115-120页 |
| VITA AND PUBLICATION | 第120-122页 |
| ACKNOWLEDGEMENTS | 第122-123页 |
