| Abstract | 第1-26页 |
| Chinese Abstract(摘要) | 第26-35页 |
| List of figures | 第35-39页 |
| List of tables | 第39-40页 |
| List of Abbreviations | 第40-41页 |
| Chapter 1 Introduction | 第41-55页 |
| ·Nanotechnology and analytical sciences | 第42-44页 |
| ·Nanotechnology, magnetisms, and magnetic materials | 第44-47页 |
| ·Overview of dissertation | 第47-53页 |
| ·Summary | 第53-55页 |
| Chapter 2 Nanobiotechnology and silicate nanoparticles | 第55-81页 |
| 2. 1 Introduction | 第55-57页 |
| ·Silicate nanoparticles preparation methods | 第57-58页 |
| ·Bulk method | 第57-58页 |
| ·Microemulsion method | 第58页 |
| ·Sol-gel chemistry | 第58-66页 |
| ·Mechanisms of sol-gel reactions | 第58-60页 |
| ·Base-catalyzed hydrolysis reactions | 第60-61页 |
| ·Acid-catalyzed hydrolysis reactions | 第61-62页 |
| ·Tin-catalyzed sol-gel reactions | 第62-64页 |
| ·Condensation reactions | 第64-66页 |
| ·Use of silicate nanoparticles in biotechnology | 第66-70页 |
| ·Quantum dots and silica coated quantum dots for recognition methods | 第66页 |
| ·Magnetic and silicate nanoparticles as enzyme carrier | 第66-67页 |
| ·Silicate nanoparticles as nanosensor | 第67-68页 |
| ·Dye-doped silicate nanoparticles as recognition tools | 第68-69页 |
| ·Magnetic silicate nanoparticles as separation device | 第69-70页 |
| ·Magnetic silicate nanoparticles as medicine carrier | 第70页 |
| ·Silicate nanoparticles as gene carriers | 第70页 |
| ·Biological separation based on antibody antigen interaction using nanoparticles | 第70-71页 |
| ·Surface modification and bioconjugation of silicate nanoparticles | 第71-79页 |
| ·Streptavidin, Avidin and Biotin | 第72-74页 |
| ·Selection of Coupling Reagents for Avidin/Streptavidin Conjugates | 第74-75页 |
| ·Immobilization of biomolecules on the surface of silicate nanoparticles according to existing functional groups chemistry | 第75-79页 |
| ·Amino group chemistry | 第75-76页 |
| ·Disulfide-coupling chemistry | 第76-77页 |
| ·Carboxyl group chemistry | 第77-78页 |
| ·Hydroxyl group Chemistry | 第78页 |
| ·Electrostatic interaction of avidin and silicate nanoparticles | 第78-79页 |
| ·Summary | 第79-81页 |
| Chapter3 Synthesis and characterization of magnetite-gold (Fe_3O_4@Au) nanoparticles and application of them in separation of ssDNA | 第81-102页 |
| ·Introduction | 第81-85页 |
| ·Experimental details | 第85-90页 |
| ·Reagents and materials | 第85-86页 |
| ·Instruments | 第86页 |
| ·Preparation of magnetic-gold coated nanoparticles | 第86-87页 |
| ·Preparation capture ssDNA-magnetic-gold nanoparticles bioconjugate for proposed separation | 第87-90页 |
| ·Formation of the magnetic-gold nanoparticles bioconjugate | 第87-88页 |
| ·Principle and choose of target ssDNA for separation | 第88-90页 |
| ·Results | 第90-98页 |
| ·Characterization of synthesized magnetic-gold nanoparticles(Fe_3O_4@Au) | 第90-92页 |
| ·Transmission Electron Microscope(TEM), size and zeta potential | 第90-91页 |
| ·Structure of magnetic-gold nanoparticles(XRD) | 第91页 |
| ·Optical Properties(UV-Visible spectrum) | 第91-92页 |
| ·Separation principle and preparation of designed probes | 第92-94页 |
| ·Separation principle | 第92-93页 |
| ·Preparation of the magnetic-gold DNA bioconjugation | 第93-94页 |
| ·Separation of target and mismatches ssDNA using prepared probes | 第94-98页 |
| ·Separation efficiency and extraction ability based on magnetic-gold DNA bioconjugate | 第94-95页 |
| ·Selectivity of the separation method based magnetic-gold probe | 第95-97页 |
| ·Reuse of the magnetic-gold probes for ssDNA separation | 第97页 |
| ·Investigation of interfering different molar ratios of 1-mimatch with complementary target ssDNA | 第97-98页 |
| ·Novel properties of magnetic-gold nanoparticles as a fast recognition device of target and mismatches ssDNA based on the UV-Visible spectroscopy | 第98-101页 |
| ·Summary | 第101-102页 |
| Chapter4 An efficient method for Recovery of target ssDNA based on Amino-modified silica coated magnetic nanoparticles (ASMNPs) | 第102-118页 |
| ·Introduction | 第102-104页 |
| ·Reagents and instruments | 第104-105页 |
| ·Chemicals and materials | 第104-105页 |
| ·Instruments | 第105页 |
| ·Experimental details | 第105-108页 |
| ·Preparation of amino-modified silica coated magnetic nanoparticles | 第105-106页 |
| ·Characterization of amino-modified silica coated magnetic nanoparticles | 第106页 |
| ·Bio-modification of the amino-modified silica coated magnetic nanoparticles | 第106-107页 |
| ·Recovery of the target ssDNA(Ⅰ) | 第107-108页 |
| ·Confirmation of the recovery | 第108页 |
| ·Results | 第108-117页 |
| ·Characterization of amino-modified silica coated magnetic nanoparticles | 第108-110页 |
| ·Bio-modification of the amino-modified silica coated magnetic nanoparticles | 第110-112页 |
| ·Recovery of target ssDNA by using of amino-modified silica coated magnetic nanoparticles | 第112-116页 |
| ·Application of this method | 第116-117页 |
| ·Summary | 第117-118页 |
| Chapter5 Magnetically Assisted Chemical Separation, principles, methods and applications | 第118-131页 |
| ·Introduction | 第118-119页 |
| ·Principles of MACS | 第119-124页 |
| ·Magnetic particles for MACS usage | 第120页 |
| ·Interaction of metal ions with particles | 第120-122页 |
| ·Magnetic flocculation | 第122-123页 |
| ·Synergistic effect in magnetic separation | 第123-124页 |
| ·High gradient magnetic separation | 第124-127页 |
| ·Structure of High Gradient Magnetic Separation (HGMS) Unit | 第125-126页 |
| ·Separation of materials in HGMS unit | 第126页 |
| ·Magnetic separators for HGMS | 第126页 |
| ·Application of HGMS | 第126-127页 |
| ·Advantages of MACS | 第127-128页 |
| ·Applications of MACS | 第128-130页 |
| ·Summary | 第130-131页 |
| Chapter6 Spectrophotometeric Determination of Ultra-trace Beryllium after Separation and Preconcentration Using Quinalizarine Modified Magnetic Microparticles | 第131-145页 |
| ·Introduction | 第131-133页 |
| ·Experimental | 第133-135页 |
| ·Chemicals | 第133页 |
| ·Apparatus | 第133页 |
| ·Procedure | 第133-135页 |
| ·Results and discussion | 第135-144页 |
| ·Photometric determination applicability and precision | 第135页 |
| ·Modification effect on extraction efficiency | 第135-136页 |
| ·Effect of microparticles amount | 第136-137页 |
| ·Effect of pH | 第137-138页 |
| ·Equilibrium time | 第138-139页 |
| ·Effect of ionic strength | 第139-140页 |
| ·Maximum capacity of microparticles | 第140页 |
| ·Stripping of modified microparticles in experiment conditions | 第140-141页 |
| ·Stability of modified microparticles in experiment conditions | 第141-142页 |
| ·Selectivity | 第142-143页 |
| ·Real sample analysis and analytical performance | 第143-144页 |
| ·Certified reference material analysis | 第144页 |
| ·Summary | 第144-145页 |
| Chapter7 Novel separation and preconcentration of trace amounts of copper (Ⅱ) in water samples based on neocuproine modified magnetic microparticles | 第145-159页 |
| ·Introduction | 第145-147页 |
| ·Experimental | 第147-149页 |
| ·Chemicals | 第147-148页 |
| ·Apparatus | 第148页 |
| ·Procedure | 第148-149页 |
| ·Results and discussion | 第149-158页 |
| ·Effect of amount of extractant | 第149页 |
| ·Effect of microparticles amount | 第149-151页 |
| ·Effect of amount of reducing agent | 第151-152页 |
| ·Effect of pH | 第152页 |
| ·Equilibrium time | 第152-153页 |
| ·Effect of ionic strength | 第153页 |
| ·Maximum capacity of microparticles and partition coefficient | 第153-154页 |
| ·Stripping and stability of modified microparticles in experiment conditions | 第154-155页 |
| ·Selectivity | 第155-156页 |
| ·Real sample analysis and analytical performance | 第156-157页 |
| ·Certified reference material analysis | 第157-158页 |
| ·Summary | 第158-159页 |
| Conclusion and Suggestion for future works | 第159-162页 |
| References | 第162-187页 |
| Publication from this thesis | 第187-188页 |
| Acknowledgements | 第188-190页 |
