| ABSTRACT | 第8-11页 |
| List of Abbreviations | 第12-14页 |
| Part I Review of literature | 第14-32页 |
| Chapter I A cross talk between cell death and life: Protein repair, recyclingand degradation | 第14-32页 |
| ABSTRACT | 第14页 |
| 1.1. PLANT STRESS RESPONSE | 第14-15页 |
| 1.2. HEAT SHOCK PROTEINS AS MOLECULAR CHAPERONES | 第15-18页 |
| 1.3. HEAT SHOCK FACTORS | 第18-21页 |
| 1.4. REGULATION OF HEAT SHOCK RESPONSE | 第21-22页 |
| 1.5. HSBP AS HSF REGULATOR | 第22-24页 |
| 1.6. BAG AS CO-CHAPERONES | 第24-25页 |
| 1.7. UNFOLDED PROTEIN RESPONSE AND AUTOPAGY | 第25-26页 |
| 1.8. ATG6/BECLIN1 PROTEINS | 第26-27页 |
| 1.10. RECENT RESEARCH ADVANCES IN RICE ON HSR | 第27-29页 |
| 1.11. STUDY OBJECTIVES | 第29-32页 |
| PART Ⅱ EXPERIMENTS | 第32-116页 |
| Chapter II Heat Shock Factor Binding Protein 1 and 2 as negativeregulators of heat shock response in rice | 第32-76页 |
| ABSTRACT | 第32-33页 |
| 2.1. INTRODUCTION | 第33-35页 |
| 2.2. MATERIALS AND METHODS | 第35-45页 |
| 2.2.1. Plant materials, growth conditions and treatments | 第35页 |
| 2.2.2. Bioinformatic analysis of OsHSBP1 and OsHSBP2 | 第35-36页 |
| 2.2.3. RNA extraction and first-strand cDNA synthesis | 第36页 |
| 2.2.4. Gene cloning and vector construction | 第36-37页 |
| 2.2.5. Semi-quantitative RT-PCR | 第37页 |
| 2.2.6. Quantitative real-time RT-PCR | 第37页 |
| 2.2.7. Sub-cellular localization | 第37-39页 |
| 2.2.8. Trans-activation and self-binding assay through yeast two-hybrid | 第39-40页 |
| 2.2.9. Transgenic rice development and identification | 第40页 |
| 2.2.10. Determination of antioxidant (CAT, POD and SOD) activity | 第40-42页 |
| 2.2.11. Identification of OsHSBP1 and OsHSBP2 mutants | 第42页 |
| 2.2.12. Phenotypic data | 第42-43页 |
| 2.2.13. Primers | 第43-45页 |
| 2.3. RESULTS | 第45-71页 |
| 2.3.1. OsHSBP1 and OsHSBP2 are functional homologs among plants and animals | 第45-50页 |
| 2.3.2. Cloning of target gene | 第50-53页 |
| 2.3.3. Expression analysis of OsHSBP1 and OsHSBP2 in rice tissues | 第53页 |
| 2.3.4. Expression analysis of OsHSBP1 and OsHSBP2 under heat stress | 第53-55页 |
| 2.3.5. Subcellular localization of OsHSBP1 and OsHSBP2 | 第55-57页 |
| 2.3.6. Trans-activation and self-binding assay of OsHSBP1 and OsHSBP2 | 第57-59页 |
| 2.3.7. Vector construction for over-expression and knock-down | 第59-60页 |
| 2.3.8. Confirmation of transgenic and mutants | 第60-62页 |
| 2.3.9. Thermotolerance assay | 第62-64页 |
| 2.3.10. Analysis of antioxidant activity | 第64-66页 |
| 2.3.11. Expression analysis of antoxidatnt (CAT and POD) and heat specific HSP genes in transgenic lines | 第66-68页 |
| 2.3.12. Premature seed abortion | 第68-71页 |
| 2.4. DISCUSSION | 第71-76页 |
| Chapter IIII dentification and characterization of The Bcl-2-associatedathanogene (BAG) family in rice | 第76-94页 |
| ABSTRACT | 第76-77页 |
| 3.1. INTRODUCTION | 第77-78页 |
| 3.2. MATERIALS AND METHODS | 第78-80页 |
| 3.2.1 Plant materials, growth conditions and stress treatments | 第78页 |
| 3.2.2 Identification of BAG family in rice | 第78-79页 |
| 3.2.3 Multiple sequence alignment and phylogenetic relationship | 第79页 |
| 3.2.4 Protein structure model | 第79页 |
| 3.2.5 "Digital Northern" analysis of BAG gene expression in rice | 第79页 |
| 3.2.6 Expression analysis of OsBAG family in rice based on microarray data | 第79-80页 |
| 3.2.7 Expression of OsBAG gene family in rice byreal-time RT-PCR | 第80页 |
| 3.3. Results | 第80-90页 |
| 3.3.1. Identification of BAG family in rice | 第80-81页 |
| 3.3.2. Genomic organization, chromosomal distribution and subcellular localization | 第81-82页 |
| 3.3.3. Alignment and phylogenetic relationship | 第82-85页 |
| 3.3.4. Comparison of the rice BAG BDs with template model | 第85-86页 |
| 3.3.5. Digital northern analysis | 第86-87页 |
| 3.3.6. Expression analysis of OsBAG family in rice based on microarray data | 第87-89页 |
| 3.3.7. Expression pattern of six OsBAG genes in rice organs | 第89页 |
| 3.3.8 Expression analysis of six OsBAG genes in rice seedlings under heat stress | 第89-90页 |
| 3.4. DISCUSSION | 第90-94页 |
| Chapter IV Identification and characterization of ATG6 / Beclin-1 homologsin rice | 第94-116页 |
| ABSTRACT | 第94页 |
| 4.1. INTRODUCTION | 第94-96页 |
| 4.2. MATERIALS AND METHODS | 第96-98页 |
| 4.2.1. Plant materials, growth conditions and stress treatments | 第96页 |
| 4.2.2. Identification of ATG6 domain in rice | 第96页 |
| 4.2.3. Multiple sequence alignment and phylogenetic relationship | 第96-97页 |
| 4.2.4. Test for selection and functional divergence analysis | 第97页 |
| 4.2.5. Expression analysis of OsA TG6 gene family and co-expressed genes inrice based on microarray data | 第97页 |
| 4.2.6. Quantitative real-time PCR | 第97-98页 |
| 4.2.7. Analysis of stress-related cis-acting elements | 第98页 |
| 4.3. Results | 第98-113页 |
| 4.3.1. ATG6 homologs in rice genome | 第98-103页 |
| 4.3.2. Evolution of ATG6 family | 第103-106页 |
| 4.3.3. Expression profile of OsATG6 genes | 第106-108页 |
| 4.3.4. Stress related Cis-acting elements and co-expressed genes | 第108-113页 |
| 4.4. Discussion | 第113-116页 |
| SUMMARY | 第116-118页 |
| MAIN FINDINGS | 第118-120页 |
| APPENDIX | 第120-127页 |
| REFERENCES | 第127-141页 |
| LIST OF PUBLICATIONS | 第141-142页 |
| ACKNOWLEDGEMENT | 第142页 |
