| Abstract | 第5-8页 |
| Chapter 1. Introduction | 第13-32页 |
| 1.1 Overview | 第13-16页 |
| 1.2 Ontogenesis of main structure in Decapod Penaeidae | 第16-23页 |
| 1.3 Immune responses of Cyclophilin A and Proliferating cell nuclear antigen in Decapod Penaeidae | 第23-25页 |
| 1.4 Basic biology of Litopenaeus vannamei | 第25-31页 |
| 1.5 Aims and Objects | 第31-32页 |
| Chapter 2. Ontogenesis of digestive system in Litopenaeus vannamei | 第32-49页 |
| 2.1 Introduction | 第32-33页 |
| 2.2 Materials and Methods | 第33-34页 |
| 2.3 Results | 第34-45页 |
| 2.3.1 Histological genesis | 第34-42页 |
| 2.3.2 Histochemistry | 第42-45页 |
| 2.4 Discussion | 第45-48页 |
| 2.5 Conclusion | 第48-49页 |
| Chapter 3. Early development of nervous system in Litopenaeus vannamei | 第49-58页 |
| 3.1 Introduction | 第49-50页 |
| 3.2 Materials and Methods | 第50-51页 |
| 3.3 Results | 第51-55页 |
| 3.3.1 Genesis and differentiation of brain | 第51页 |
| 3.3.2 Genesis and differentiation of olfactory lobes and optic ganglions | 第51-52页 |
| 3.3.3 Genesis of ganglion | 第52-55页 |
| 3.4 Discussion | 第55-58页 |
| Chapter 4. Genesis of Haematopoietic tissue and Cytochemical Study of Haemocytes in Litopenaeus vannamei. | 第58-73页 |
| 4.1 Introduction | 第58-60页 |
| 4.2 Materials and Methods | 第60-63页 |
| 4.2.1 Animal | 第60-61页 |
| 4.2.2 Histology of haematopoetic tissue (HPT) | 第61页 |
| 4.2.3 Cytochemical assays of haemocytes | 第61-63页 |
| 4.2.3.1 Haemocyte harvesting | 第61-62页 |
| 4.2.3.2 Giemsa’s dye | 第62页 |
| 4.2.3.3 Wright-Giemsa | 第62页 |
| 4.2.3.4 Periodic acid Schiff | 第62页 |
| 4.2.3.5 Sudan Black B staining | 第62-63页 |
| 4.2.3.6 Prophenoloxidase | 第63页 |
| 4.3 Results | 第63-70页 |
| 4.3.1 Genesis of Haematopoetic tissue | 第63页 |
| 4.3.2 Structure of HPT in Adult | 第63-64页 |
| 4.3.3 Haemocyte morphology and cytochemical assay | 第64-70页 |
| 4.4 Discussion | 第70-73页 |
| Chapter 5. cDNA cloning and expression of Cyclophilin A (LvCypA) in white leg shrimp (Litopenaeus vannamei) after challenge with Vibrio anguillarum | 第73-90页 |
| 5.1 Introduction | 第73-75页 |
| 5.2 Materials and Methods | 第75-79页 |
| 5.2.1 Experimental Shrimps | 第75-76页 |
| 5.2.2 Bacterial Challenge | 第76页 |
| 5.2.3 Total RNA extraction | 第76页 |
| 5.2.4 First strand cDNA synthesis | 第76-77页 |
| 5.2.5 Cloning of full length LvCyPA and sequencing | 第77-78页 |
| 5.2.6 Real time analysis of LvCypA mRNA expression | 第78-79页 |
| 5.2.7 Statistical Analysis | 第79页 |
| 5.3 Results | 第79-86页 |
| 5.3.1 Cloning and sequence analysis of cDNA endocing LvCypA | 第79-83页 |
| 5.3.2 Pylogenetic analysis of LvCypA | 第83-84页 |
| 5.3.3 Tissue distribution of LvCypA mRNA | 第84-85页 |
| 5.3.4 Expression level of control and bacterial challenged groups | 第85-86页 |
| 5.4 Discussion | 第86-90页 |
| Chapter 6. cDNA cloning of Proliferating of cell nuclear antigen (PCNA)and expression in white leg shrimp (Litopenaeus vannamei) after challenge with Vibrio anguillarum | 第90-101页 |
| 6.1 Introduction | 第90-91页 |
| 6.2 Materials and Methods | 第91-92页 |
| 6.2.1 Experimental Shrimps | 第91页 |
| 6.2.2 Bacterial Challenge | 第91页 |
| 6.2.3 Total RNA extraction | 第91-92页 |
| 6.2.4 Cloning of full length LvPCNA and sequencing | 第92页 |
| 6.2.5. Statistical analysis | 第92页 |
| 6.2.6 Real time analysis of LvPCNA mRNA expression | 第92页 |
| 6.3 Results | 第92-98页 |
| 6.3.1 Full length and phylogenetic analysis of LvPCNA | 第92-97页 |
| 6.3.2 Tissue distribution | 第97页 |
| 6.3.3 Expression level LvPCNA in tissues bacterial challange | 第97-98页 |
| 6.4 Discussion | 第98-101页 |
| References | 第101-130页 |
| Acknowledgements | 第130-132页 |
| Publications | 第132-133页 |
| List of Abbreviations | 第133-134页 |
