| 摘要 | 第3-5页 |
| Abstract | 第5-6页 |
| List of abbreviations | 第10-11页 |
| Chapter 1 General introduction | 第11-15页 |
| 1. The links between father-offspring bonding and drug abuse | 第11-12页 |
| 2. Neurochemical substrate associated with father-offspring bonding and drug abuse | 第12-14页 |
| 3. The scope of our study | 第14-15页 |
| Chapter 2 Preference for cocaine- versus pups-associated cuesdifferentially alter D2R,OTR and V1AR expression in the NAcc and MeA,and serum hormone in mandarin vole fathers | 第15-35页 |
| 1. Introduction | 第15-20页 |
| 1.1. The link between oxytocin,vasopressin,dopamine and paternal behavior as well drug abuse | 第15-18页 |
| 1.2. The reward circuit associated with paternal behavior and drug abuse | 第18-19页 |
| 1.3. Conditioned place preference | 第19-20页 |
| 2. Materials and methods | 第20-25页 |
| 2.1. Subject | 第20页 |
| 2.2. Drug administration | 第20-21页 |
| 2.3. Conditioned place preference test | 第21-23页 |
| 2.3.1. Apparatus | 第21页 |
| 2.3.2. Pretest | 第21-22页 |
| 2.3.3. Conditioning with saline | 第22页 |
| 2.3.4. Conditioning with cocaine | 第22页 |
| 2.3.5. Conditioning with pups | 第22-23页 |
| 2.4. Western blotting | 第23-24页 |
| 2.4.1. Serum collection and tissue punches from the NAcc and MeA | 第23页 |
| 2.4.2. Protein extraction and western blot analysis | 第23-24页 |
| 2.5. Serum OT,AVP,Estradiol and Testosterone assays | 第24页 |
| 2.6. Statistical analyses | 第24-25页 |
| 3. Results | 第25-26页 |
| 3.1. Conditioning place preference | 第25页 |
| 3.2. The protein levels of D2R,OTR and AVP1aR | 第25页 |
| 3.3. Concentrations of serum OT,AVP,E2 and T | 第25-26页 |
| 4. Discussion | 第26-31页 |
| 4.1. D2R,OTR and V1AR expression associated with a preference for cocaine or pups | 第26-29页 |
| 4.2. OT,AVP,E2 and T associated with a preference for cocaine or pups | 第29-31页 |
| Figure caption | 第31-35页 |
| Chapter 3 The effects of paternal deprivation on cocaine-inducedbehavioral sensitization and alteration of Oxytocin expression in MandarinVoles | 第35-53页 |
| 1. Introduction | 第35-37页 |
| 2. Materials and methods | 第37-41页 |
| 2.1. Subjects | 第37页 |
| 2.2. Treatments | 第37-38页 |
| 2.3. Open-field test | 第38页 |
| 2.4. Social interaction tests | 第38-39页 |
| 2.5. Tissue collection and immunochemistry | 第39-40页 |
| 2.6. Serum estradiol and OT assays | 第40-41页 |
| 2.7. Statistical analyses | 第41页 |
| 3. Results | 第41-43页 |
| 3.1. Open-field test | 第41页 |
| 3.2. Female-female social interaction test | 第41-42页 |
| 3.3. The expression of OT | 第42-43页 |
| 3.4. Concentrations of serum estradiol and OT | 第43页 |
| 4. Discusson | 第43-47页 |
| 4.1. Effects of cocaine exposure on anxiety-like behavior and locomotivity in PC and PD voles | 第43-44页 |
| 4.2. Effects of cocaine on sociability in PC and PD voles | 第44-45页 |
| 4.3. Effects of OT and estradiol | 第45-47页 |
| Figure captions | 第47-53页 |
| Chapter 4 Summary and future directions | 第53-55页 |
| Reference | 第55-69页 |
| Acknowledgements | 第69-71页 |
| List of publication | 第71页 |
