| 摘要 | 第5-7页 |
| Abstract | 第7-9页 |
| Part Ⅰ Introduction | 第10-28页 |
| 1. Chemically modified electrodes | 第10-15页 |
| 1.1 Introduction of chemically modified electrodes | 第10页 |
| 1.2 Fabrication of chemically modified electrodes | 第10-14页 |
| 1.2.1 Covalent binding method | 第11-12页 |
| 1.2.2 Adsorption method | 第12-13页 |
| 1.2.3 Thin polymer film method | 第13-14页 |
| 1.3 Application of chemically modified electrodes in analytical chemistry | 第14-15页 |
| 2. Some pollutants and protein | 第15-18页 |
| 2.1 Thrombin | 第15-16页 |
| 2.2 Cadmvum(Ⅱ) | 第16-17页 |
| 2.3 4-Nitrophenol | 第17-18页 |
| References | 第18-28页 |
| Part Ⅱ Main research works | 第28-78页 |
| Section 1. Study on the chronocoulometric aptasensor based on gold nanoparticles as signal amplification strategy and its application for determination of thrombin | 第28-42页 |
| 1. Experamental | 第29-32页 |
| 1.1. Reagent | 第29-30页 |
| 1.2. Apparatus | 第30-31页 |
| 1.3. Preparation of gold nanoparticles | 第31页 |
| 1.4. Functionalization of gold nanoparticles with DNA | 第31页 |
| 1.5. Fabrication of the aptasensor and thrombin detection | 第31-32页 |
| 1.6. Surface density of aptamers | 第32页 |
| 2. Results and discussion | 第32-38页 |
| 2.1. Characterization of the aptasensor | 第32-35页 |
| 2.2. Optimization of surface density of aptamers | 第35-36页 |
| 2.3. Sensitive detection of thrombin | 第36-37页 |
| 2.4. Detection selectivity for thrombin | 第37-38页 |
| 3. Conclusions | 第38-39页 |
| References | 第39-42页 |
| Section 2. Sensitive determination of Cd(Ⅱ) by square wave anodic stripping voltammetry with in situ bismuth and tin modified carbon paste electrode | 第42-57页 |
| 1. Experimental | 第43-45页 |
| 1.1. Reagent | 第43页 |
| 1.2. Apparatus | 第43页 |
| 1.3. Preparation of carbon paste electrodes | 第43-44页 |
| 1.4. Procedure | 第44-45页 |
| 2. Results and discussion | 第45-52页 |
| 2.1. Square-wave voltammetric response of Cd(Ⅱ) at the bismuth-tin composite film electrode | 第45-46页 |
| 2.2. Optimization of experimental conditions | 第46-50页 |
| 2.3. Calibration curve | 第50-51页 |
| 2.4. Interference study | 第51页 |
| 2.5. Analytical application to tap water sample | 第51-52页 |
| 3. Conclusions | 第52-54页 |
| References | 第54-57页 |
| Section 3. Fabrication of graphene-gold nanocomposites by electrochemical co-reduction and their electrocatalytic activity toward 4-nitrophenol oxidation | 第57-78页 |
| 1. Experimental | 第58-60页 |
| 1.1. Reagent | 第58-59页 |
| 1.2. Apparatus | 第59页 |
| 1.3. Preparation of graphene-gold nanocomposite film modified glassy carbon electrode | 第59-60页 |
| 1.4. Analytical procedure | 第60页 |
| 2. Results and discussion | 第60-72页 |
| 2.1. Characterizations of the modified electrodes | 第60-62页 |
| 2.2. Electrochemical oxidation behavior of 4-nitrophenol at the modified electrode | 第62-64页 |
| 2.3. Optimization of experimental parameters | 第64-67页 |
| 2.4. Effect of scan rate | 第67页 |
| 2.5. Determination of effective surface area of the electrode | 第67-69页 |
| 2.6. Calibration curve | 第69-70页 |
| 2.7. Interference study | 第70-71页 |
| 2.8. Analytical application to river water sample | 第71-72页 |
| 3. Conclusions | 第72-73页 |
| References | 第73-78页 |
| Part Ⅲ Conclusions | 第78-80页 |
| 致谢 | 第80-82页 |
| Appendix: Papers published during the graduate | 第82页 |
