| List of abbreviation | 第10-12页 |
| 摘要 | 第12-15页 |
| Abstract | 第15-17页 |
| Chapter 1 Introduction | 第19-44页 |
| 1.1 The plant immune system | 第19-22页 |
| 1.2 Pathogen-triggered post-translational modifications | 第22-25页 |
| 1.3 Ubiquitination during plant immune signalling | 第25-32页 |
| 1.4 Phytophthora infestans | 第32-41页 |
| 1.5 Research scope of this thesis | 第41-44页 |
| Chapter 2 Materials and methods | 第44-54页 |
| 2.1 Plant materials and growth condition | 第44页 |
| 2.2 P. infestans strains and maintenance | 第44页 |
| 2.3 P. infestans infection assay | 第44-46页 |
| 2.4 Cloning and protein fusions | 第46-48页 |
| 2.5 Quantitative RT-PCRs | 第48页 |
| 2.6 Flg22 treatment and gene expression assays | 第48-49页 |
| 2.7 TRV-based VIGS in N. benthamiana | 第49页 |
| 2.8 Agrobacterium-mediated transient expression and infection assays | 第49-50页 |
| 2.9 Western analysis | 第50页 |
| 2.10 Potato transformation | 第50-51页 |
| 2.11 Confocal microscopy | 第51页 |
| 2.12 Recombinant protein purification | 第51-52页 |
| 2.13 E3 ubiquitin ligase activity assay | 第52页 |
| 2.14 Yeast-two-hybrid | 第52-53页 |
| 2.15 Co-immunoprecipitation analyses | 第53-54页 |
| Chapter 3 U-box E3 ubiquitin ligase PUB17 acts in the nucleus to promote specific immunepathways triggered by Phytophthora infestans | 第54-92页 |
| 3.1 Introduction | 第54-56页 |
| 3.2 Results | 第56-85页 |
| 3.2.1 St PUB17 functions as an E3 ubiquitin ligase | 第56-58页 |
| 3.2.2 P. infestans colonization is enhanced by RNAi St PUB17 in potato | 第58-61页 |
| 3.2.3 Silencing of Nb PUB17 by VIGS in Nicotiana benthamiana increases susceptibilityto P. infestans | 第61-65页 |
| 3.2.4 PUB17 is involved in the activation of PTI | 第65-66页 |
| 3.2.5 PUB17 acts as a positive regulator of specific cell death events | 第66-71页 |
| 3.2.6 PUB17 localizes in the nucleolus | 第71-74页 |
| 3.2.7 PUB17 functions in the nucleolus | 第74-80页 |
| 3.2.8 Nuclear localisation of PPUB17 is required for its regulatory role in basalimmunity to Phytophthora infestans | 第80-85页 |
| 3.3 Discussion | 第85-92页 |
| Chapter 4 PUB17 is targeted and degraded by the BTB/POZ domain protein POB1 tosuppress plant immunity | 第92-113页 |
| 4.1 Introduction | 第92-95页 |
| 4.2 Results | 第95-108页 |
| 4.2.1 Sequencing analysis of POB1 | 第95页 |
| 4.2.2 POB1 suppresses basal defense against P.infestans | 第95-99页 |
| 4.2.3 Nuclear localisation of POB1 is a prerequisite for its regulatory role | 第99-103页 |
| 4.2.4 St PUB17 was destabilized by POB1 | 第103-108页 |
| 4.3 Discussion | 第108-113页 |
| Chapter 5 A Phytophthora infestans effector Pi SFI3 targets an E3 Ubiquitin ligase UBKthat is a positive regulator of the flg22-induced immune response | 第113-138页 |
| 5.1 Introduction | 第113-115页 |
| 5.2 Results | 第115-135页 |
| 5.2.1 Pi SFI3 enhances P. infestans colonization | 第115-118页 |
| 5.2.2 Pi SFI3 attenuates flg22-induced immunity | 第118-120页 |
| 5.2.3 Cell death triggered by INF1 or Cf4/Avr4 were not attenuated by Pi SFI3 | 第120-122页 |
| 5.2.4 Pi SFI3 targets UBK in the nucleus | 第122-126页 |
| 5.2.5 UBK contributes to reduce P. infestans colonization | 第126-132页 |
| 5.2.6 UBK is a positive regulator of flg22-induced immune response | 第132-135页 |
| 5.3 Discussion | 第135-138页 |
| Chapter 6 General discussion and future directions | 第138-153页 |
| 6.1 General discussion | 第138-147页 |
| 6.2 Future directions | 第147-153页 |
| References | 第153-174页 |
| Appendix 1: List of figures | 第174-177页 |
| Appendix 2: List of tables | 第177-181页 |
| Papers arising during this Ph D | 第181-182页 |
| Acknowledgements | 第182-185页 |
| 致谢 | 第185-187页 |
| About the author | 第187-190页 |
