| CHPATER 1 Establishment and characterization of the mammary gland epithelial cell line inYanbian dairy goat | 第1-30页 |
| Abstract | 第10-11页 |
| 1 Introduction | 第11-13页 |
| 2 Materials and Methods | 第13-17页 |
| ·Medium preparation for cell culture | 第13页 |
| ·Isolation and culture of DGMECs | 第13-16页 |
| ·Biological characteristics of DGMECs | 第16页 |
| ·Statistic analysis of data | 第16-17页 |
| 3 Results | 第17-25页 |
| ·Morphology of DGMECs | 第17-18页 |
| ·Growth curve of DGMECs | 第18-20页 |
| ·Average adherence and survival rate of cell | 第20-22页 |
| ·Doubling time of cell population | 第22-24页 |
| ·The mitotic index of DGMECs | 第24-25页 |
| 4 Discussion | 第25-27页 |
| References | 第27-30页 |
| CHAPTER 2 Establishment of an embryonic stem cell line from blastocyst embryos in mouse | 第30-59页 |
| Abstract | 第31-32页 |
| 1 Introduction | 第32-34页 |
| 2 Materials and Methods | 第34-44页 |
| ·Preparation of MEF | 第34-38页 |
| ·Mitomycin C-inactivation of MEF cells | 第38-39页 |
| ·Preparation of freeze stock | 第39-40页 |
| ·Mice, embryos and isolation of ES cells | 第40-43页 |
| ·Statistic analysis of data | 第43-44页 |
| 3 Results and analysis | 第44-52页 |
| ·Characteristics of the MEF isolated from different embryonic mouse age | 第44-45页 |
| ·Influence of different trypsinase concentration on isolation of MEF | 第45页 |
| ·The growth curve of the mouse MEF | 第45-46页 |
| ·The mitotic index of the MEFs | 第46-47页 |
| ·Cryopreservation and recovery of MEF | 第47-48页 |
| ·The concentration and treatment time of MMC treatment on the MEFs | 第48-50页 |
| ·Effect of ES cell isolate by different treatment time with MMC | 第50-51页 |
| ·Effect of different MEF concentration on ICM and ES cells | 第51页 |
| ·Effecs of LIF concentration on the growth of mouse ES cell | 第51-52页 |
| 4 Discussion | 第52-55页 |
| References | 第55-59页 |
| CHAPTER 3 Establishment of Embryonic Stem Cells From NT Blastocystin Yanbian Goat | 第59-81页 |
| ABSTRACT | 第60-61页 |
| 1 INTRODUCTION | 第61-63页 |
| 2 Materials and Methods | 第63-71页 |
| ·Preparation of MEF | 第63页 |
| ·Goat Fetal Fibroblast Feeder Cell Line | 第63-64页 |
| ·Embryo Collection | 第64-67页 |
| ·ES Cell Isolation | 第67-68页 |
| ·Differential Staining | 第68-70页 |
| ·Statistic analysis of data | 第70-71页 |
| 3 RESULTS | 第71-74页 |
| ·Growth curve of goat fetal fibroblast Cell | 第71页 |
| ·Embryo Culture and ES Cells Derivation | 第71-74页 |
| 4 DISCUSSION | 第74-77页 |
| REFERENCES | 第77-81页 |
| CHAPTER 4 Method of Establishing Embryonic Stem (ES) Cell line | 第81-95页 |
| 1 Established ES cell culture system method | 第82-85页 |
| ·The feeder layer culture system | 第82-84页 |
| ·Feeder-free culture systems | 第84-85页 |
| 2 Embryo collection | 第85-86页 |
| 3 ICM's acquisition | 第86-89页 |
| ·Direct separation method | 第86页 |
| ·Immune separation method | 第86-87页 |
| ·Heat shock method | 第87-88页 |
| ·Delayed implantation method | 第88-89页 |
| ·ES cell tissue blocks culture | 第89页 |
| ·Feeder layer inverted culture | 第89页 |
| 4 Endoderm group's Discretion | 第89-90页 |
| 5 Identification of ES cells | 第90-92页 |
| REFERENCE | 第92-95页 |
| CHAPTER 5 The Normal Microenvironment In Mammary Gland Development | 第95-112页 |
| Abstract | 第96-97页 |
| Introduction | 第97-101页 |
| Role of Normal Mammary Microenvironment in Tissue Engineering | 第101-105页 |
| Conclusions and Future Perspectives | 第105-106页 |
| References | 第106-112页 |
| 致谢 | 第112-115页 |
| 附录：攻读学位期间发表的论文 | 第115-116页 |
