| 中文摘要 | 第1-4页 |
| Abstract | 第4-9页 |
| 1. INTRODUCTION | 第9-39页 |
| ·Background | 第9-13页 |
| ·Effect of endocrine disruption on the male reproductive system | 第13-14页 |
| ·DEHP as an environmental endocrine disruptor | 第14-28页 |
| ·Introduction to DEHP | 第14-19页 |
| ·Animal Studies | 第19-22页 |
| ·Summary of DEHP effects (rat) from literature | 第22-23页 |
| ·Non-human Primate Studies | 第23-24页 |
| ·Human Studies | 第24-28页 |
| ·Developmental toxicity testing: an important tool in human health risk assessment | 第28-31页 |
| ·Toxicogenomics: a powerful instrument in toxicity testing | 第31-33页 |
| ·MicroRNA-based toxicogenomics | 第33-37页 |
| ·Role of miRNAs in the testes | 第35-37页 |
| ·Purpose and methodology of this study | 第37-39页 |
| ·Purpose and objectives | 第37-38页 |
| ·Methodology | 第38-39页 |
| 2. MATERIALS AND METHODS | 第39-52页 |
| ·Test compounds | 第39页 |
| ·Preparation of animals | 第39页 |
| ·Dose selection and administration of chemicals | 第39-40页 |
| ·Isolation of gonads from fetuses at necropsy | 第40-41页 |
| ·Serum testosterone analysis | 第41-44页 |
| ·Histopathological analysis of the testis | 第44-46页 |
| ·miRNA expression changes determined by Microarray | 第46-49页 |
| ·RNA Extraction | 第46页 |
| ·MicroRNA profiling through microarray | 第46-49页 |
| ·miRNAs expression changes corroborated by QRT-PCR | 第49-50页 |
| ·Target prediction | 第50-51页 |
| ·Statistical Analysis | 第51-52页 |
| 3. RESULTS | 第52-70页 |
| ·Observations from pregnant females and fetuses at necropsy | 第52-56页 |
| ·Fetal serum testosterone concentration | 第56-57页 |
| ·Histopathology of the fetal testis | 第57-60页 |
| ·miRNA expression changes determined by microarray | 第60-61页 |
| ·Target prediction | 第61-62页 |
| ·Analysis of selected miRNAs by qRT-PCR | 第62-65页 |
| ·Analysis of validated targets | 第65-70页 |
| 4. DISCUSSION | 第70-84页 |
| ·Affinity with results from previous studies | 第70-74页 |
| ·External evaluation of fetuses | 第70-71页 |
| ·Testosterone assessment comparison | 第71-74页 |
| ·Histopathological evaluation of testes | 第74页 |
| ·Microarray and PCR analysis of dysregulated miRNAs and target prediction | 第74-77页 |
| ·miR-195 | 第75-76页 |
| ·miR-185 | 第76-77页 |
| ·miR-451 | 第77页 |
| ·Target pathway analysis | 第77-81页 |
| ·Relevance of findings from target analysis | 第81-84页 |
| 5. CONCLUSIONS | 第84-87页 |
| ·Summary and Conclusions | 第84-85页 |
| ·Suggestions for further investigation | 第85-87页 |
| 6. REFERENCES | 第87-98页 |
| 7. ACKNOWLEDGEMENTS | 第98-99页 |
| 8 APPENDIX | 第99-104页 |
| Curriculum vitae | 第104页 |
