| Abstract | 第7-10页 |
| List of Abbreviations | 第11-13页 |
| Part Ⅰ.REVIEW OF LITERATURE | 第13-30页 |
| 1.1.Structure,expression and functions of somatostatin | 第13-15页 |
| 1.2.Relationship between somatostatin and other hormones | 第15-26页 |
| 1.2.1.Regulation of the GH:somatostatin and GHRH | 第15-23页 |
| 1.2.1.a.Mechanism of actions of somatostatin and its receptors | 第16-18页 |
| 1.2.1.b.Mechanisms of action of GHRH its receptors | 第18-23页 |
| 1.2.2.Feedback regulation of GH secretion | 第23-25页 |
| 1.2.3.Somatostatin and reproductive hormones | 第25-26页 |
| 1.3.Genes expression in granulosa cells | 第26-28页 |
| 1.4.Phylogeny of smatostatin in mammals | 第28-30页 |
| Part Ⅱ.EXPERIMENTS | 第30-67页 |
| A.Effect of somatostatin transfection on hormone secretion and gene expression related to growth and fertility of bovine ovarian cells | 第30-44页 |
| 1.1.Introduction | 第30-31页 |
| 1.2.Material and methods | 第31-35页 |
| 1.2.1.Plasmid construct,granulosa cells culture,transient transfection and GFP-expression study | 第31-32页 |
| 1.2.2.RNA extraction,reverse transcription and Real-Time PCR quantification | 第32-34页 |
| 1.2.3.Radioimmunoassay(RIA) and statistical analysis | 第34页 |
| 1.2.4.Development of oocyte co-cultured with transfected GCs | 第34-35页 |
| 1.2.4.a.Oocyte Maturation | 第34-35页 |
| 1.2.4.b.In vitro fertilization and embryo culture | 第35页 |
| 1.3.Results | 第35-40页 |
| 1.4.Discussion | 第40-44页 |
| B.Phylogenetic history of somatostatin in mammals | 第44-67页 |
| 1.1 Introduction | 第44-45页 |
| 1.2.Material & methods | 第45-48页 |
| 1.2.1.Identification of putative somatostatin and somatostatin receptors within the draft genomes of D.rerio,Carassius auratus,X.tropicalis,G.gallus,Monodelphis domestica,Homo sapiens,Sus scrofa,Bos taurus,Mus musculus,Rattus norvegicus,Canis lupus famili | 第45-46页 |
| 1.2.2.Pairwise homology of putative somatostatin and somatostatin receptors | 第46页 |
| 1.2.3.Phylogenetic analysis of somatostatin and somatostatin receptors | 第46-48页 |
| 1.3.Results | 第48-56页 |
| 1.3.1.Somatostatin and somatostatin receptors in fishes,amphibians and aves | 第50-54页 |
| 1.3.2.Somatostatin and somatostatin receptors in mammals | 第54-56页 |
| 1.3.3.Comparative genomic analysis of invertebrates failed to identify somatostatin or its receptors | 第56页 |
| 1.4.Discussion | 第56-67页 |
| 1.4.1.Evolutionary history of somatostatin and its receptors | 第56-67页 |
| Part Ⅲ.CONSOLIDATE DISCUSSION | 第67-74页 |
| Part Ⅳ.SUMMARY | 第74-76页 |
| Part Ⅴ.LITERATURE CITED | 第76-98页 |
| Publications Record | 第98-99页 |
| ACKNOWLEDGEMENT | 第99页 |
