| 1 INTRODUCTION | 第22-31页 |
| 2 LITERATURE REVIEW | 第31-47页 |
| 2． 1 CLASSICAL GENETIC STUDIES ON QUANTITATIVE TRAITS | 第31-32页 |
| 2． 2 QTL MAPPING | 第32-36页 |
| 2． 2． 1 Morphological Markers | 第32-33页 |
| 2． 2． 2 Protein markers | 第33-34页 |
| 2． 2． 3 DNA markers | 第34-36页 |
| 2． 3 STUDIES ON DNA PROFILING AND GENETIC RELATIONSHIP／DISTANCE USING MOLECULAR MARKERS | 第36-40页 |
| 2． 4 COMPARISON OF DIFFERENT MOLECULAR MARKER ASSAYS | 第40-42页 |
| 2． 5 CONSTRUCTION OF GENETIC MAPS | 第42-44页 |
| 2． 6 ANALYSIS OF BULKED SEGREGANTS | 第44-47页 |
| 3 MATERIALS AND METHODS | 第47-56页 |
| 3． 1 MATERIALS | 第47-49页 |
| 3． 1． 1 Biology of Silkworm | 第47页 |
| 3． 1． 2 Silkworm rearing | 第47页 |
| 3． 1． 3 Silkworm strains | 第47-48页 |
| 3． 1． 4 Population for bulk segrigants | 第48-49页 |
| 3． 2 METHODS | 第49-56页 |
| 3． 2． 1 DNAisolation | 第49-50页 |
| 3． 2． 2 Quantitation of genomic DNA | 第50页 |
| 3． 2． 3 Source of oligonucleotide primers | 第50页 |
| 3． 2． 4 RAPD-PCR amplification | 第50-51页 |
| 3． 2． 5 Separation and detection of PCR product | 第51页 |
| 3． 2． 6 RFLP analysis | 第51页 |
| 3． 2． 7 Probe preparation and immunological detection | 第51-52页 |
| 3． 2． 8 Plasmid isolation | 第52页 |
| 3． 2． 9 Data analysis | 第52-56页 |
| 4 RESULTS AND DISCUSSION | 第56-104页 |
| 4． 1 PERFORMANCE OF INBRED LINES OF B．20A AND C．NICHI | 第56-58页 |
| 4． 1． 1 Confirmation of homozygosity of inbred lines by random clones． | 第56页 |
| 4． 1． 2 Confirmation of homozygosity of inbred lines by random primers | 第56-58页 |
| 4． 2 CONSTRUCTION OF PARTIAL GENOMIC LIBRARY | 第58-61页 |
| 4． 2． 1 Screening genomic libraries for low copy DNA inserts | 第58-61页 |
| 4． 3 GENETIC RELATIONSHIPS AMONG DIVERSE SILKWORM STRAINS USING RAPD AND RFLP | 第61-69页 |
| 4． 3． 1 Optimization of amplification condition of silkworm DNA | 第61-65页 |
| 4． 3． 1． 1 Sensitivity of the RAPD assay | 第61-62页 |
| 4． 3． 1． 2 Inheritance analysis | 第62页 |
| 4． 3． 1． 3 Marker identification | 第62-64页 |
| 4． 3． 1． 4 Relationships among the strains | 第64-65页 |
| 4． 3． 2 Degree of RFLP polymorphism | 第65-69页 |
| 4． 3． 2． 1 Type of restriction enzyme to generate RFLP | 第66-68页 |
| 4． 3． 2． 2 Confirmation of polymorphism by chi-square analysis | 第68-69页 |
| 4． 4 DISCUSSION | 第69-83页 |
| 4． 4． 1 Genetic variability within varieties | 第71-83页 |
| 4． 5 COMPARISION OF GENETIC VARIABILITY AMONG SILKWORM STRAINS DETECTED BY RAPD AND RFLP MARKER ASSAYS | 第83-85页 |
| 4． 6 DISSCUSSION | 第85-87页 |
| 4． 7 ANALYSIS OF BUKLED SEGREGANTS（BSA）AND NEAR ISOGENIC LINES（NILs）TO IDENTIFY MOLECULAR MARKERS LINKED WITH COCOON WEIGHT AND COCOON SHELL WEIGHT | 第87-91页 |
| 4． 7． 1 Detection of polymorphism in bulked segregant analysis | 第89页 |
| 4． 7． 2 Performance of the NearIsogenic Lines | 第89-90页 |
| 4． 7． 3 Detection of polymorphism between the parents | 第90-91页 |
| 4． 8 DISCUSSION | 第91-104页 |
| 5 SUMMARY | 第104-108页 |
| 5． 1 GENETIC RELATIONSHIP AMONG DIVERSE SILKWORM STRAINS USING RAPD AND RFLP MARKERS | 第104-105页 |
| 5． 2 COMPARISON OF RAPDs WITH RFLPs | 第105-106页 |
| 5． 3 ANALYSIS OF BULKED SEGREGANT（BSA）AND NEAR ISOGENIC LINES（NIL） | 第106-108页 |
| 6 GLOSSARY | 第108-111页 |
| 7 REFERENCES | 第111-123页 |
