| 摘要 | 第1-6页 |
| ABSTRACT | 第6-8页 |
| LIST OF ABBREVIATIONS | 第8-12页 |
| INTRODUCTION | 第12-15页 |
| The main aims | 第13页 |
| Objective | 第13页 |
| Scientific innovation | 第13-15页 |
| PART Ⅰ.THEORETICAL FIELD | 第15-24页 |
| ·Carthamus tinctorius species | 第15页 |
| ·Carthamus tinctorius L.in Traditional Medicine | 第15-16页 |
| ·Phytochemical investigation of Carthamus tinctorius L. | 第16-23页 |
| ·Pharmacological and Clinical Studies of Carthamus tinctorius L. | 第23-24页 |
| PART Ⅱ.MATERIALS AND METHODS | 第24-30页 |
| ·Plant materials | 第24页 |
| ·Instruments and reagents | 第24-30页 |
| ·Isolation of compounds from plant material | 第25-26页 |
| ·UPLC-DAD-ESI-MS analysis | 第26页 |
| ·DPPH scavenging activity | 第26-27页 |
| ·Preparation of stock solutions,calibration standard solutions and QCsolutions | 第27页 |
| ·Preparation of safflower solution for quantification | 第27-28页 |
| ·Preparation of plasma samples | 第28页 |
| ·Method validation | 第28-29页 |
| ·Pharmacokinetic study in rat plasma | 第29-30页 |
| PART Ⅲ. RESULTS AND DISCUSSION | 第30-67页 |
| ·Isolated compounds 1-12, from C. tinctorius L | 第30-55页 |
| ·Structure elucidation of compound 1 (hydroxysafflor yellow A) | 第31-33页 |
| ·Structure determination of compound 2 (6-hydroxykaempferol3,6-diglucoside) | 第33-35页 |
| ·Structure determination of compound 3 (6-hydroxykaempferol 3,6,7-triglucoside) | 第35-37页 |
| ·Structure determination of compound 4 (6-hydroxykaempferol3-rutinoside-glucoside) | 第37-40页 |
| ·Structure determination of compound 5 (kaempferol-3-O-rutinoside) | 第40-42页 |
| ·Structure determination of compound 6 (kaempferol-3-O-glucoside) | 第42-43页 |
| ·Structure determination of compound 7 (isoquercetin) | 第43-44页 |
| ·Structure determination of compound 8 (chlorogenic acid) | 第44-45页 |
| ·Structure determination of compound 9 (syringin) | 第45-47页 |
| ·Structure determination of compound 10 (protocatechuic acid) | 第47-49页 |
| ·Structure determination of compound 11 (p-coumaric acid) | 第49-50页 |
| ·Structure determination of compound 12(8Z-decaene-4,6-diyne-1-O-β-D-glucopranoside) | 第50-55页 |
| ·Antioxidant activity of the components of Carthamus tinctorius L. byDPPH radical scavenging assay | 第55-56页 |
| ·Quantification and pharmacokinetic studies of safflower constituentsin rat | 第56-67页 |
| ·Optimized UPLC-MS/MS conditions | 第56-57页 |
| ·Method validation | 第57-63页 |
| ·Contents of 11 constituents in safflower samples | 第63-64页 |
| ·Pharmacokinetics of 5 safflower constituents after oral administration of safflower powder | 第64-67页 |
| Conclusions | 第67-68页 |
| References | 第68-77页 |
| 攻读博士学位期间完成的论文 | 第77-78页 |
| 致谢 | 第78页 |
