| Acknowledgements | 第1-10页 |
| Abbreviation | 第10-11页 |
| Abstract | 第11-16页 |
| Abstract in Chinese | 第16-19页 |
| Chapter Ⅰ Progress on important aspects in somatic embryogenesis and plant regeneration with particular reference to Vitis vinifera L | 第19-38页 |
| ·Somatic embryogenesis in grapevine | 第19-20页 |
| ·Concept and characteristics of plant somatic embryogenesis | 第20-24页 |
| ·Uses of somatic embryogenesis in crop improvement | 第24-25页 |
| ·Regeneration of genetically transformed plants | 第24页 |
| ·Polyploid line production | 第24-25页 |
| ·Germplasm preservation | 第25页 |
| ·Factors crucial for SE induction | 第25-32页 |
| ·Explant type and endogenous hormones | 第26-27页 |
| ·Plant growth regulators | 第27-29页 |
| ·Sugars and other stress factors | 第29-31页 |
| ·Light conditions | 第31-32页 |
| ·Conversion of somatic embryos into plants | 第32-33页 |
| ·Genetic characterization of regenerants | 第33-37页 |
| ·Origin of somatic embryos | 第33-34页 |
| ·Somaclonal variation and detection in vitro tissue culture | 第34-37页 |
| ·Somaclonal variation | 第34-35页 |
| ·Molecular markers for somaclonal variation | 第35-37页 |
| ·Conclusion and study objectives | 第37-38页 |
| Chapter Ⅱ Somatic embryogenesis and plant regeneration from immature zygotic embryos | 第38-49页 |
| ·Introduction | 第38-39页 |
| ·Material and methods | 第39-40页 |
| ·Plant materials and preparation of immature zygotic embryos | 第39页 |
| ·Callus induction | 第39页 |
| ·Somatic embryo production | 第39页 |
| ·Somatic embryo conversion and plant regeneration | 第39-40页 |
| ·Culture condition | 第40页 |
| ·Histological and histological study in somatic embryogenesis | 第40页 |
| ·Results | 第40-46页 |
| ·Callius induction | 第40-41页 |
| ·Somatic embryogenesis | 第41页 |
| ·Embryo conversion and plant regeneration | 第41-43页 |
| ·Morphological and histological in somatic embryogenesis | 第43页 |
| ·Cytological configuration in somatic embryogenesis | 第43-46页 |
| ·Discussion | 第46-48页 |
| ·Conclusion | 第48-49页 |
| Chapter Ⅲ Monitoring of genetic fidelity of plants regenerated from somatic embryos | 第49-62页 |
| ·Introduction | 第49页 |
| ·Material and methods | 第49-53页 |
| ·Chromosome analysis of regenerated plantlets | 第49-50页 |
| ·Flow cytometric analysis of regenerated plantlets | 第50页 |
| ·RAPD marker | 第50-53页 |
| ·DNA extraction | 第50-52页 |
| ·PCR amplification | 第52-53页 |
| ·Statistical analysis | 第53页 |
| ·Result | 第53-56页 |
| ·Analysis of the ploidy stability of regenerated plants | 第53-55页 |
| ·Monitoring of genetic fidelity by RAPD | 第55-56页 |
| ·Discussion | 第56-60页 |
| ·Ploidy level assayed by FCM | 第56-57页 |
| ·Somaclonal variation in somatic embryogenesis | 第57-60页 |
| ·Conclusion | 第60-62页 |
| Chapter Ⅳ Tetraploid Regeneration from diploid somatic embryos in grapevine | 第62-75页 |
| ·Introduction | 第62-63页 |
| ·Materials and methods | 第63-65页 |
| ·Plant materials and culture condition | 第63页 |
| ·Colchicine treatment | 第63-64页 |
| ·Conversion of somatic embryo and plant regeneration | 第64页 |
| ·Chromosome analysis of regenerated plantlets | 第64页 |
| ·Ploidy level determination by FCM | 第64-65页 |
| ·Evaluation of leaf stomata characteristics | 第65页 |
| ·Results | 第65-71页 |
| ·Somatic embryogenesis | 第65-66页 |
| ·Effects of colchicine on somatic embryos | 第66-69页 |
| ·Chromosomes analysis of regenerated plantlets | 第69页 |
| ·Ploidy level determination by FCM | 第69-70页 |
| ·Evaluation of leaf stomata characteristics | 第70-71页 |
| ·Discussion | 第71-73页 |
| ·Colchicine as mutagen | 第71-72页 |
| ·Induction of tetraploid | 第72-73页 |
| ·Conclusion | 第73-75页 |
| References | 第75-94页 |
| Curriculum vitae | 第94-96页 |
