| 中文文摘 | 第1-12页 |
| Abstract | 第12-16页 |
| Chapter one Introduction | 第16-39页 |
| ·Transport of fatty acid | 第16-19页 |
| ·LCFA uptake into heart and skeletal muscle | 第16-18页 |
| ·LCFA uptake in health and disease | 第18-19页 |
| ·FATPs family | 第19-30页 |
| ·FATP1 | 第20-24页 |
| ·FATP4 | 第24-25页 |
| ·Other members of FATP family | 第25-27页 |
| ·Regulation of FATP expression | 第27-28页 |
| ·Mechanism of FATP action | 第28-30页 |
| ·Real-time PCR | 第30-35页 |
| ·Principle of real-time PCR process | 第30-31页 |
| ·Advantage and limitation of real-time PCR | 第31-33页 |
| ·Fields of real-time PCR application | 第33-35页 |
| ·Future development of real-time PCR | 第35页 |
| ·Gene polymorphism | 第35-38页 |
| ·Single nucleotide polymorphism(SNP) | 第36-37页 |
| ·Methods for SNP analysis | 第37-38页 |
| ·The purpose and meaning of the study | 第38-39页 |
| Chapter Two Molecular cloning and character of chicken FATP4 gene | 第39-60页 |
| ·Introduction | 第39-40页 |
| ·Material and methods | 第40-45页 |
| ·RNA extraction | 第40页 |
| ·RNA integrity and purity | 第40页 |
| ·Reverse Transcription(RT) | 第40-41页 |
| ·Primer design and PCR amplification | 第41-42页 |
| ·PCR product purification | 第42-43页 |
| ·Cloning of FATP4 gene | 第43-45页 |
| ·Sequence analysis | 第45页 |
| ·Results | 第45-57页 |
| ·Data extraction of cDNA sequence and nucleotide sequence analysis | 第45-47页 |
| ·Analysis of the homologies for nucleotide sequences and animo acid sequences | 第47-51页 |
| ·Character of the chicken FATP4gene | 第51-52页 |
| ·Analysis of FATP4 signal peptide | 第52-53页 |
| ·Analysis of transmembrane domain of chicken FATP4 | 第53-54页 |
| ·Analysis of glyeosylation site | 第54-55页 |
| ·Analysis of phosphorylation | 第55页 |
| ·Analysis of hydrophobicity | 第55-56页 |
| ·Predict the second structure of chicken FATP4 protein | 第56-57页 |
| ·Phylogenetic tree constructed from FATP4 | 第57页 |
| ·Discussion | 第57-60页 |
| Chapter three Expression of FATP4 in chickens by quantitative RT-PCR | 第60-77页 |
| ·Introduction | 第60-61页 |
| ·Material and Methods | 第61-63页 |
| ·Animals and tissue collection | 第61页 |
| ·RNA extraction and purification | 第61页 |
| ·Primer design | 第61-62页 |
| ·First strand cDNA synthesis | 第62页 |
| ·Quantitative RT-PCR | 第62-63页 |
| ·Statistical analysis | 第63页 |
| ·Results | 第63-73页 |
| ·Quality of RNA and PCR detection | 第63-64页 |
| ·Standard curves and melting curves of FATP4 and house-keeping gene | 第64-65页 |
| ·The expression of FATP1 and FATP4 in different breeds | 第65-66页 |
| ·Expression of FATP1 and FATP4 in different tissues at the same time | 第66-70页 |
| ·Ontogenic expression of chicken FATP1 and FATP4 | 第70-73页 |
| ·The ration of relative quantity of FATP1 gene to relative quantity of FATP4 gene at different growth points | 第73页 |
| ·Discussion | 第73-77页 |
| Chapter four Association of SNP in FATP1 gene with carcass traits and abdominal fat | 第77-118页 |
| ·Introduction | 第77页 |
| ·Material and Methods | 第77-82页 |
| ·Sample collection | 第77-78页 |
| ·Phenotyping for Carcass Traits | 第78页 |
| ·DNA extraction | 第78-79页 |
| ·DNA yield and Purity | 第79页 |
| ·Primer design and amplification by PCR | 第79-80页 |
| ·PCR-SSCP | 第80-81页 |
| ·Gene sequencing | 第81页 |
| ·Statistical analysis | 第81-82页 |
| ·Results | 第82-114页 |
| ·SNP detection in FATP1 gene | 第82-85页 |
| ·Genotype and allele frequencies in chicken populations of 70d and 91d | 第85-89页 |
| ·Comparison of carcass traits among different genotypes of 70d and 91d | 第89-92页 |
| ·Construction of haplotypes and their associations with chicken carcass traits of 70d and 91d | 第92-97页 |
| ·Frequencies of genotypes and alleles of all chicken | 第97-100页 |
| ·Association of SNPs in FATP1 gene with carcass traits among different breeds | 第100-107页 |
| ·Association between genotypes and carcass traits of all chicken | 第107-109页 |
| ·Construction of haplotypes and their frequencies in all population | 第109-110页 |
| ·Association of the haplotypes with carcass traits in all chicken population | 第110-114页 |
| ·Discussion | 第114-118页 |
| ·FATP1 SNP genotype | 第114-115页 |
| ·Association of SNP in FATP1 gene with certain economical traits | 第115-116页 |
| ·Association of haplotype with carcass traists | 第116-118页 |
| Chapter five Association of SNP in FATP4 gene with carcass traits and abdominal fat | 第118-156页 |
| ·Introduction | 第118-119页 |
| ·Material and Methods | 第119-121页 |
| ·Sample collection | 第119页 |
| ·DNA extraction and primers design | 第119-120页 |
| ·PCR-SSCP and gene sequencing | 第120-121页 |
| ·Statistical analysis | 第121页 |
| ·Results | 第121-153页 |
| ·SNP detection in FATP4 gene | 第121-124页 |
| ·Genotype and allele frequencies in chicken populations of 70d and 91d | 第124-131页 |
| ·Association of SNP with carcass traits of chicken 70d and 91d | 第131-135页 |
| ·Construction of haplotypes and their associations with chicken carcass traits | 第135-141页 |
| ·Frequencies of genotypes and alleles of all chicken | 第141-144页 |
| ·Association of FATP4 SNPs with carcass traits | 第144-147页 |
| ·Construction of haplotypes and their frequencies in all population | 第147-148页 |
| ·Association of the haplotypes with carcass traits | 第148-153页 |
| ·Discussion | 第153-156页 |
| Chapter Six General Conclusions | 第156-159页 |
| References | 第159-172页 |
| Acknowledgement | 第172-173页 |
| Articles published and submitted | 第173-174页 |
| Appendix Ⅰ | 第174页 |
| Appendix Ⅱ | 第174-176页 |
| Appendix Ⅲ 中文目录 | 第176-181页 |
| Appendix Ⅳ 第章中文概述 | 第181-189页 |
