| Abstract | 第5-7页 |
| 中文摘要 | 第8-11页 |
| Abbreviations/Acronyms | 第11-13页 |
| Chapter Ⅰ:Review:Judge it by deeds, not words--multifaceted roles of HP1γ | 第13-51页 |
| 1.1 Introduction | 第14-15页 |
| 1.2 HP1 family members | 第15-17页 |
| 1.3 HP1γ is involved in multiple biological processes | 第17-24页 |
| 1.3.1 Heterochromatin structure | 第17页 |
| 1.3.2 Senescence | 第17-18页 |
| 1.3.3 DNA damage | 第18-20页 |
| 1.3.4 Cell cycle | 第20-21页 |
| 1.3.5 Cell differentiation | 第21-23页 |
| 1.3.6 Cancer development | 第23-24页 |
| 1.4 Versatile molecular functions of HP1γ | 第24-34页 |
| 1.4.1 The role of HP1γ on transcriptional initiation | 第25-27页 |
| 1.4.2 The role of HP1γ on transcriptional elongation | 第27-30页 |
| 1.4.3 The role of HP1γ on RNA splicing | 第30-32页 |
| 1.4.4 The role of HP1γ on transcription termination | 第32-34页 |
| 1.5 The explanation for versatile molecular functions of HP1γ | 第34-35页 |
| 1.6 Regulation of HP1γ | 第35-36页 |
| 1.6.1 Transcriptional regulation | 第35页 |
| 1.6.2 Post-translation modification | 第35-36页 |
| Conclusion and perspectives | 第36-39页 |
| Reference | 第39-51页 |
| Chapter Ⅱ:HP1γ is required for prostate cancer progression through HP1γ/miR-451a /c-Myc feedback loop | 第51-101页 |
| Summary | 第52-53页 |
| Introduction | 第53-55页 |
| Materials and Methods | 第55-63页 |
| Results | 第63-92页 |
| 2.1 HP1γ is overexpressed in prostate cancer | 第63-66页 |
| 2.1.1 HP1γ is overexpressed in human prostate cancer | 第63-65页 |
| 2.1.2 HP1γ is potential prognosis marker for prostate cancer | 第65-66页 |
| Conclusion | 第66页 |
| 2.2 HP1γ is required for prostate cancer progression | 第66-71页 |
| 2.2.1 Loss of HP1γ decreases cell growth in vitro | 第66-67页 |
| 2.2.2 Loss of HP1γ inhibits tumorigenesis in vivo | 第67-68页 |
| 2.2.3 Loss of HP1γ induces G2/M arrest and apoptosis | 第68-70页 |
| Conclusion | 第70-71页 |
| 2.3 Overexpression of HP1γ is not sufficient for prostate cancer progression | 第71-72页 |
| 2.4 Loss of HP1γ regulates cancer-related miRNAs | 第72-77页 |
| 2.4.1 HP1γ directly suppresses miR-451 a | 第72-75页 |
| 2.4.2 miR-451a is an oncogenic microRNA in prostate cancer | 第75-76页 |
| 2.4.3 miR-451a mediates the roles of HP1γ on prostate cancer | 第76-77页 |
| Conclusion | 第77页 |
| 2.5 HPly partially mediates c-Myc oncogenic role on prostate cancer | 第77-84页 |
| 2.5.1 HPly is regulated by c-Myc | 第77-80页 |
| 2.5.2 c-Myc directly regulates HP1γ through E-box element | 第80-82页 |
| 2.5.3 HP1γ is required for c-Myc effects on prostate cancer | 第82-84页 |
| Conclusion | 第84页 |
| 2.6 HP1γ/miR-451a/c-Myc loop | 第84-89页 |
| 2.6.1 c-Myc regulates miR-451 a through HP1γ | 第84-85页 |
| 2.6.2 c-Myc does not interact with HP1γ | 第85-86页 |
| 2.6.3 miR-451a directly targets 3'UTR of c-Myc | 第86-87页 |
| 2.6.4 HP1γ conversely regulates c-Myc through miR-451 a | 第87-88页 |
| 2.6.5 The loop | 第88页 |
| Conclusion | 第88-89页 |
| 2.7 HP1γ/miR-451a/c-Myc correlation in clinical data | 第89-92页 |
| 2.7.1 c-Myc, HP1γ is correlated in prostate cancer samples in protein level | 第89-90页 |
| 2.7.2 c-Myc, HP1γ and miR-451 a is correlated in prostate cancer samples | 第90-92页 |
| Summary and discussion | 第92-95页 |
| Reference | 第95-101页 |
| 致谢 | 第101-103页 |
| Publications | 第103-106页 |
