| List of abbreviations | 第10-11页 |
| 摘要 | 第11-15页 |
| Abstract | 第15-18页 |
| Chapter 1: Introduction | 第19-29页 |
| 1.1 Background | 第19-21页 |
| 1.2 Plant tissue culture | 第21-22页 |
| 1.3 Role of nutrient media composition in plant tissue culture | 第22页 |
| 1.4 Influence of nitrogen and its sources in plant tissue culture | 第22-23页 |
| 1.5 Influence of reduced nitrogen in plant tissue culture conditions | 第23-25页 |
| 1.6 Silver nitrate (AgNO_3) a growth promoter | 第25-26页 |
| 1.7 Plant tissue culture in okra | 第26-27页 |
| 1.8 Project aims and objectives | 第27-29页 |
| Chapter 2: Reduced nitrogen level in culture medium, results in the formation ofembryogenic calluses with high-frequency regeneration in okra(Abelmoschus esculentus L.) | 第29-53页 |
| 2.1: Introduction | 第31-33页 |
| 2.2: Methodology | 第33-37页 |
| 2.2.1 Seed material and culture conditions | 第33-34页 |
| 2.2.2 Modification of MS medium with different nitrogen levels | 第34-35页 |
| 2.2.3 Callus induction | 第35页 |
| 2.2.4 Callus growth and development | 第35页 |
| 2.2.5 Media composition for shoot Induction | 第35-36页 |
| 2.2.6 Shoot elongation | 第36页 |
| 2.2.7 Rooting and acclimatization of plantlets | 第36-37页 |
| 2.2.8 Statistical analysis | 第37页 |
| 2.3 Results | 第37-48页 |
| 2.3.1 Influence of plant growth regulators on callus induction | 第37-38页 |
| 2.3.2 Influence of plant growth regulators on callus growth and development | 第38-41页 |
| 2.3.3 Influence of modified MS medium on shoot induction | 第41-42页 |
| 2.3.4 Shoot elongation | 第42-46页 |
| 2.3.5 Rooting and acclimatization of plantlets | 第46-48页 |
| 2.4 Discussion | 第48-52页 |
| 2.5 Conclusion | 第52-53页 |
| Chapter3: Silver nitrate (AgNO_3) boosted high-frequency multiple shootregeneration from cotyledonary node explants of okra (Abelmoschusesculentus L.) | 第53-71页 |
| 3.1 Introduction | 第55-57页 |
| 3.2 Methodology | 第57-59页 |
| 3.2.1 Silver nitrate (AgNO_3) stock solution preparation | 第57页 |
| 3.2.2 Plant material and seed germination | 第57页 |
| 3.2.3 Culture medium and conditions | 第57-58页 |
| 3.2.4 Explant preparation and shoot induction | 第58页 |
| 3.2.5 Multiple shoots induction | 第58页 |
| 3.2.6 Shoot elongation | 第58页 |
| 3.2.7 Rooting of regenerated shoots and acclimatization | 第58-59页 |
| 3.2.8 Statistical analysis | 第59页 |
| 3.3 Results | 第59-66页 |
| 3.3.1 Effects of plant growth regulator on shoot bud induction | 第59页 |
| 3.3.2 Shoot multiplication and proliferation | 第59-62页 |
| 3.3.3 Shoot elongation | 第62-63页 |
| 3.3.4 In vitro rooting and acclimatization | 第63-66页 |
| 3.4 Discussion | 第66-69页 |
| 3.5 Conclusion | 第69-71页 |
| Chapter 4: General discussion, conclusion and recommendations | 第71-75页 |
| References | 第75-85页 |
| Appendices | 第85-112页 |
| Publications | 第112-113页 |
| Acknowledgements | 第113页 |
