| 摘要 | 第6-7页 |
| Abstract | 第7-8页 |
| Abbreviation | 第15-17页 |
| CHAPTER-1 GENERAL INTRODUCTION | 第17-38页 |
| 1. INTRODUCTION | 第17-24页 |
| 1.1 Veterinary drugs in Animal production | 第17-19页 |
| 1.2 Effects of antibiotic | 第19-21页 |
| 1.3 Applications of the veterinary drugs | 第21-24页 |
| 2 REGULATION IN CHINA | 第24-28页 |
| 2.1 Banned drugs and substance in feed and animal productions in China | 第24-25页 |
| 2.2 Food safety standards, rules and regulation in China | 第25-26页 |
| 2.3 Feed safety supervision and assurance schemes | 第26-28页 |
| 3. BACKGROUND OF THE RESEARCH | 第28-35页 |
| 3.1 Simultaneous determination of multi-class veterinary drugs | 第28-30页 |
| 3.2 Overview the extraction and different matrices for multi-class method | 第30-32页 |
| 3.3 Validation of the method Guidelines for the implementation of decision 2002/657/EC | 第32-35页 |
| 4 Objectives of the research | 第35-38页 |
| 4.1 The specific objectives of the research | 第35页 |
| 4.2 Challenges of the research | 第35-36页 |
| 4.3 Scope of the study | 第36-38页 |
| CHAPTER-2 DEVELOPMENT OF MULTI-CLASS VETERINARY DRUGS METHOD IN FEEDING STUFF | 第38-63页 |
| 1. Summary | 第38页 |
| 2 Introduction | 第38-39页 |
| 3 MATERIALS AND METHODS | 第39-42页 |
| 3.1 Chemicals and reagents | 第39页 |
| 3.2 Standards and stock solutions | 第39-40页 |
| 3.3 Instrumentation and conditions | 第40-41页 |
| 3.4 LC-MS/MS conditions | 第41页 |
| 3.5 Feed samples | 第41页 |
| 3.6 Protein precipitation | 第41-42页 |
| 3.7 Sample extraction | 第42页 |
| 3.8 Matrix match calibration | 第42页 |
| 3.9 Method validation | 第42页 |
| 4. RESULTS AND DISCUSSIONS | 第42-61页 |
| 4.1 Optimization of extraction procedure | 第42-44页 |
| 4.2 Sample purification Dispersive-SPE (D-spe) | 第44页 |
| 4.3 Optimization of the LC-MS/MS conditions | 第44-45页 |
| 4.4 Method validation | 第45-46页 |
| 4.5 Recovery | 第46页 |
| 4.6 LOD and LOQ | 第46页 |
| 4.7 Matrix effect evaluation | 第46-61页 |
| 5. CONCLUSION | 第61-63页 |
| CHAPTER-3 VALIDATION OF THE DEVELOPED METHOD | 第63-74页 |
| 1. Summary | 第63-73页 |
| 1.1 Introduction | 第63页 |
| 1.2 MATERIALS AND METHODS | 第63-64页 |
| 1.2.1 Chemicals, standard and reagents | 第64页 |
| 1.2.2 Validation protocol | 第64页 |
| 1.3 RESULTS AND DISCUSSION | 第64-73页 |
| 1.3.1 Method validation | 第64页 |
| 1.3.2 CCα and CCβ | 第64页 |
| 1.3.3 Linearity | 第64页 |
| 1.3.4 Recovery | 第64页 |
| 1.3.5 Selectivity and specificity | 第64-73页 |
| 2. CONCLUSION | 第73-74页 |
| CHAPTER-4 SIMULTANEOUS DETERMINATION OF THE 173 VETERINARY DRUG MULTI-CLASSMETHOD IN URINE | 第74-88页 |
| 1. Summary | 第74-88页 |
| 1.1 Introduction | 第74-75页 |
| 1.2 MATERIALS AND METHODS | 第75-76页 |
| 1.2.1 Chemicals, standard and reagents | 第75页 |
| 1.2.2 Instrumentation and software | 第75页 |
| 1.2.3 Matrix match calibration | 第75页 |
| 1.2.4 Beta-glucuronidase/aryl sulfate | 第75-76页 |
| 1.2.5 Urine Samples | 第76页 |
| 1.2.6 Extraction method of the urine | 第76页 |
| 1.2.7 Purification procedures | 第76页 |
| 1.2.8 Method validation | 第76页 |
| 1.3 RESULTS AND DISCUSSIONS | 第76-87页 |
| 1.3.1 Optimization of sample preparation | 第76-77页 |
| 1.3.2 Method validation | 第77页 |
| 1.3.3 Specificity | 第77页 |
| 1.3.4 Linearity | 第77页 |
| 1.3.5 Recovery and precision | 第77页 |
| 1.3.6 LOD and LOQ | 第77-78页 |
| 1.3.7 Matrix effect in urine | 第78-87页 |
| 1.4 CONCLUSION | 第87-88页 |
| CHAPTER-5 SIMULTANEOUS DETERMINATION OF THE 173 VETERINARY DRUGS MULTI-CLASS METHOD IN BLOOD | 第88-98页 |
| 1. Summary | 第88-98页 |
| 1.1 INTRODUCTION | 第88-89页 |
| 1.2 MATERIALS AND METHOD | 第89-90页 |
| 1.2.1 Chemicals, standard and reagents | 第89页 |
| 1.2.2 Instrumentation and software | 第89页 |
| 1.2.3 Matrix match calibration | 第89页 |
| 1.2.4 Blood samples | 第89页 |
| 1.2.5 Sample extraction | 第89-90页 |
| 1.2.6 Purification of blood sample | 第90页 |
| 1.2.7 Method validation | 第90页 |
| 1.3 RESULTS AND DISCUSSION | 第90-97页 |
| 1.3.1 Sample extraction optimization | 第90页 |
| 1.3.3 Method validation | 第90-91页 |
| 1.3.4 Matrix effects | 第91-97页 |
| 1.4 CONCLUSION | 第97-98页 |
| CHAPTER-6 METHOD APPLICATION ON SURVEY SAMPLES | 第98-106页 |
| 1 Introduction | 第98-106页 |
| 1.2 MATERIALS AND METHOD Chemicals, standard and reagents | 第99-100页 |
| 1.2.1 Matrix match calibration | 第99-100页 |
| 1.2.2 Instrumentation | 第100页 |
| 1.2.3 Feed samples | 第100页 |
| 1.2.4 Urine sample | 第100页 |
| 1.3 RESULTS AND DISCUSSION | 第100-104页 |
| 1.3.1 Recovery, LOD, and LOQ | 第100页 |
| 1.3.2 Application of the method on real feed samples | 第100-102页 |
| 1.3.3 Application of the method on real urine samples | 第102-104页 |
| 1.4 CONCLUSION | 第104-106页 |
| REFERENCES | 第106-113页 |
| ACKNOWLEDGEMENTS | 第113-115页 |
| Author's Biography | 第115-116页 |
