| 中文摘要 | 第1-5页 |
| Abstract | 第5-7页 |
| Abbreviations | 第7-11页 |
| Chapter 1: Review | 第11-50页 |
| ·Actin Dynamics | 第12-18页 |
| ·Actin Dynamics is regulated by actin binding proteins | 第14-16页 |
| ·ADF/Cofilin-mediated actin depolymerization | 第16-18页 |
| ·Actin-interacting protein 1 (AIP1) | 第18-24页 |
| ·Biochemical properties of AIP1 | 第19-20页 |
| ·Biological functions of AIP1 | 第20-23页 |
| ·AIP1 in Drosophila melanogaster | 第23-24页 |
| ·Morphogenesis in Drosophila Eye | 第24-31页 |
| ·Structure of Drosophila eye | 第24-25页 |
| ·Development of eye disc | 第25-27页 |
| ·Morphogenetic furrow | 第27-29页 |
| ·Pattern formation in eye disc | 第29-31页 |
| ·Adherens junction remodeling | 第31-37页 |
| ·Basic structure of adherens junctions | 第32-34页 |
| ·Regulation of adherens junctions | 第34-35页 |
| ·Adherens junctions in Drosophila retina | 第35-36页 |
| ·Adherens junction remodeling and morphogenesis | 第36-37页 |
| References | 第37-50页 |
| Chapter 2: The Drosophila AIP1 gene is required for eye development | 第50-71页 |
| Introduction | 第51-52页 |
| Results | 第52-63页 |
| ·Gene structure and molecular analysis of fir alleles | 第52-54页 |
| ·fir mutations cause rough adult eye | 第54-55页 |
| ·AIP1 is required for F-actin disassembly in eye disc | 第55-56页 |
| ·AIP1 is required for AJ reconstruction in eye discs | 第56-58页 |
| ·Other junctions are not grossly affected in flr'EY-P2 clones | 第58-61页 |
| ·Cell differentiation is delayed in flr'EY-P2 mosaic | 第61-62页 |
| ·AIP1 expression is not regulated by atonal or EGFR signaling | 第62-63页 |
| Discussion | 第63-65页 |
| Materials and Methods | 第65-68页 |
| References | 第68-71页 |
| Chapter 3: AIP1 localization and function in the Drosophila eye disc | 第71-85页 |
| Introduction | 第72-73页 |
| Results | 第73-80页 |
| ·AIP1 is colocalized with F-actin and AJ in larval eye disc | 第73-75页 |
| ·AIP1 is localized to the rhabdomere in pupal eye disc | 第75页 |
| ·AIP1 localization is distinct from that of cofilin in eye disc | 第75-76页 |
| ·tsr mutations exhibit similar phenotype as fir | 第76-77页 |
| ·Cofilin overexpression rescues fir AJ remodeling defects | 第77-79页 |
| ·flr.RNAi clones exhibits no AJ defects in epithelia that does not undergo extensive remodeling | 第79-80页 |
| Discussion | 第80-81页 |
| Materials and Methods | 第81-82页 |
| References | 第82-85页 |
| Chapter 4: Actin dynamics is essential for AJ remodeling atfer the morphogenetic furrow | 第85-95页 |
| Introduction | 第86页 |
| Results | 第86-90页 |
| ·Drugs affecting actin dynamics causes AJ remodeling defects in preclusters and clusters close to the MF | 第86-88页 |
| ·Fluorescence recovery after photobleaching (FRAP) analysis reveals preclusters closer to the MF exhibit more dynamic AJ turnover | 第88-89页 |
| ·A model showing how AIP1 works in eye epithelia | 第89-90页 |
| Discussion | 第90-92页 |
| Materials and Methods | 第92-93页 |
| References | 第93-95页 |
| 致谢 | 第95-98页 |
