| Chinese abstract | 第1-6页 |
| English abstract | 第6-10页 |
| Chapter 1 Literature review | 第10-22页 |
| 1.Microcystin pollution in China | 第10页 |
| 2.Structure and acute toxicity of microcystins | 第10-15页 |
| ·Classification of Cyanotoxins | 第10-11页 |
| ·Structure and Toxicity of Microcystins | 第11-15页 |
| ·The Molecular Structure of Microcystins | 第12-13页 |
| ·The toxicology of microcystins | 第13-15页 |
| 3.Heat Shock Protein | 第15-20页 |
| ·General Introduction to Heat Shock Protein | 第15-16页 |
| ·Heat Shock Protein 70 | 第16-20页 |
| ·Structure of HSP70 | 第17页 |
| ·Function and Regulation of HSP70 | 第17-19页 |
| ·HSP70 in Environmental Monitoring | 第19-20页 |
| 4.The background and significance of this paper | 第20-22页 |
| Chapter 2 Transcriptional Responses of HSP 70 Genes in the Liver of Three Freshwater Fishes Intraperitoneally Injected with MC-LR | 第22-50页 |
| 1.Introduction | 第22-25页 |
| 2 Materials and Methods | 第25-29页 |
| ·Materials | 第25页 |
| ·Fish and Microcystin Exposure | 第25-26页 |
| ·Total RNA Isolation from Fish Liver | 第26页 |
| ·Cloning of Partial HSP70 cDNA Sequences of Fish | 第26-27页 |
| ·Full-length cDNA Amplification of silver carp,grass carp and Nile tilapia HSP70 Genes by 5' and 3'RACEs | 第27-28页 |
| ·Quantitation of Relative Silver Carp,Grass Carp and Nile tilapia Liver HSP70 mRNA Abundance after Exposure to MC-LR | 第28页 |
| ·Statistical Analysis | 第28-29页 |
| 3 Results and Analysis | 第29-47页 |
| 4 Discussions | 第47-50页 |
| References | 第50-61页 |
| Appendix-1 Detailed procedure for RNA isolation | 第61-63页 |
| Appendix-2 Detailed procedure for reverse transcription | 第63-64页 |
| Appendix-3 Detailed procedure for ligation and transformation | 第64-66页 |
| Acknowledgements | 第66页 |
