| 摘要 | 第8-9页 |
| ABSTRACT | 第9-11页 |
| CHAPTER Ⅰ INTRODUCTION | 第12-30页 |
| 1.1 Background to the study | 第12-14页 |
| 1.2 Physical properties, production, occurrence, and uses of phenanthrene and pyrene | 第14-16页 |
| 1.3 Purpose and meaning of using phenanthrene and pyrene in microbial degradation and phytoremediationresearch | 第16-18页 |
| 1.4 Degradation mechanisms in bacteria | 第18-19页 |
| 1.5 PAH-degrading conditions in the soil environments | 第19-20页 |
| 1.6 Taxonomic properties of PAH-degrading Mycobacterium and sphingomonads | 第20-22页 |
| 1.7 Adaptive features of sphingomonads in bioremediation and Mycobacterium in phytoremediation | 第22-24页 |
| 1.8 Statement of the problem | 第24-26页 |
| 1.9 Objectives of the study | 第26-27页 |
| 1.9.1 Broad objective | 第26页 |
| 1.9.2 Specific objectives | 第26-27页 |
| 1.10 Research questions | 第27页 |
| 1.11 Justification of the study | 第27-28页 |
| 1.12 Thesis outlook | 第28-30页 |
| CHAPTER Ⅱ ISOLATION, CHARACTERIZATION AND BIODEGRADATION OFPHENANTHRENE BY PHENANTHRENE-DEGRADING SPHINGOBIUM SP. RS1 | 第30-40页 |
| 2.1 Materials and methods | 第30-33页 |
| 2.1.1 Materials and reagents | 第30-31页 |
| 2.1.2 Isolation, maintenance and identification of Sphingobium sp. RS1 | 第31-32页 |
| 2.1.3 Phenanthrene biodegradation assay | 第32页 |
| 2.1.4 Statistical analysis | 第32-33页 |
| 2.2 Results | 第33-36页 |
| 2.2.1 Isolation and characterization of phenanthrene-degrading bacteria | 第33-35页 |
| 2.2.2 Biodegradation of phenanthrene | 第35-36页 |
| 2.3 Discussion and summary | 第36-40页 |
| CHAPTER Ⅲ COMETABOLISM OF PHENANTHRENE AND PYRENE BYPHENANTHRENE-DEGRADING SPHINGOBIUM SP. RS1 | 第40-48页 |
| 3.1 Materials and methods | 第40-42页 |
| 3.1.1 Materials and reagents | 第40-41页 |
| 3.1.2 Pyrene co-metabolism by isolate RS1 | 第41-42页 |
| 3.1.3 Statistical analysis | 第42页 |
| 3.2 Results and discussion | 第42-46页 |
| 3.3 Summary | 第46-48页 |
| CHAPTER IV UTILIZING PAH-DEGRADING MYCOBACTERIUM TO REDUCEPHENANTHRENE AND PYRENE CONTAMINATION IN RYEGRASS (LOLIUM MULTIFLORUMLAM.) | 第48-64页 |
| 4.1 Materials and methods | 第48-53页 |
| 4.1.1 Chemicals | 第48-50页 |
| 4.1.2 Ryegrass colonization | 第50-51页 |
| 4.1.3 Enzyme activity | 第51-52页 |
| 4.1.4 PAH analysis | 第52页 |
| 4.1.5 Statistical analysis | 第52-53页 |
| 4.2 Results | 第53-58页 |
| 4.2.1 Performance of endophyte-mediated uptake of PAHs by plants | 第53-57页 |
| 4.2.2 Influence of inoculated strain ATCC PYR-GCK on the enzyme activity in plants | 第57-58页 |
| 4.3 Discussion | 第58-62页 |
| 4.4 Summary | 第62-64页 |
| CHAPTER V CONCLUSIONS | 第64-68页 |
| 5.1 Conclusions | 第64-65页 |
| 5.2 Recommendations | 第65-66页 |
| 5.3 Main creative points of this work | 第66-68页 |
| REFERENCES | 第68-82页 |
| ACKNOWLEDGEMENTS | 第82-86页 |
| PUBLICATIONS | 第86页 |
