| ABSTRACT | 第6-7页 |
| ABBREVIATIONS | 第8-9页 |
| 1 INTRODUCTION | 第9-29页 |
| 1.1 Introduction to cotton | 第9-10页 |
| 1.2 Utilization of cotton | 第10页 |
| 1.3 Verticillium wilt in cotton | 第10-11页 |
| 1.4 Genetics of cotton resistance to Verticillium wilt | 第11-12页 |
| 1.5 Traditional genetic breeding of resistance to verticillium wilt | 第12-14页 |
| 1.6 Genetic engineering of cotton resistance to Verticillium wilt | 第14-15页 |
| 1.7 Plant hormones and their role in plant growth and stress response | 第15-16页 |
| 1.8 Jasmonic acid signal pathway and plant immune system | 第16-19页 |
| 1.9 Myelocytomatosis(MYCs) | 第19-20页 |
| 1.10 Gene function assay through transformation | 第20-24页 |
| 1.11 Virus-Induced Gene Silencing (VIGS) | 第24-26页 |
| 1.12 Aims of study and summary of the present research | 第26-28页 |
| 1.13 An overview of the study | 第28-29页 |
| 2 MATERIALS AND METHODS | 第29-38页 |
| 2.1 Bioinformatics studies of MYC2-Like gene | 第29页 |
| 2.2 Genotypes used in study | 第29页 |
| 2.3 Genetic transformation studies in Gossypium irsutum | 第29-35页 |
| 2.3.1 Experimental conditions | 第29-30页 |
| 2.3.2 Plant cultivation and hormone treatment | 第30页 |
| 2.3.3 MYC2-like protein expression in cotton during V.dahliae infection | 第30页 |
| 2.3.4 Identification of cDNA and Quantitative Real-time PCR analysis | 第30-31页 |
| 2.3.5 Vector construction | 第31页 |
| 2.3.6 Seedling growth,Medium preparation,and Transformation | 第31-33页 |
| 2.3.7 DNA extraction and PCR for the selection of positive plants | 第33页 |
| 2.3.8 Southern blot analysis | 第33-34页 |
| 2.3.9 Stress responsive genes expression analysis by RT-PCR | 第34页 |
| 2.3.10 Response of GhMYC2-like gene against insect feeding on transgenic cotton | 第34-35页 |
| 2.4 Virus induced gene-silencing studies in Gossypium barbedense | 第35-38页 |
| 2.4.1 Vector construction and Virus Induce in cotton | 第35-36页 |
| 2.4.2 RNA extraction and RT-PCR analysis | 第36页 |
| 2.4.3 Preparation and inoculation of V. dahliae (V991) | 第36页 |
| 2.4.4 Disease Index | 第36-37页 |
| 2.4.5 Botrytis cinerea (Gray Mold) inoculation | 第37-38页 |
| 3 RESULTS | 第38-49页 |
| 3.1 Cloning and sequence analysis of MYC2-like in cotton | 第38-40页 |
| 3.1.1 MYC2-like protein isolation and sequence analysis | 第39-40页 |
| 3.2 Expression pattern analysis of MYC2-like in cotton | 第40页 |
| 3.3 The expression pattern analysis of GhMYC2-like in cotton under hormone treatments | 第40-41页 |
| 3.4 The expression of MYC2-like is upregulated upon V. dahliae infection | 第41-42页 |
| 3.5 Genetic Transformation and characterization of MYC2-like in Gossypium hirsutum | 第42-44页 |
| 3.5.1 Regeneration of transgenic cotton plants | 第42页 |
| 3.5.2 Selection of positive transgenic cotton plants | 第42-43页 |
| 3.5.3 Southern blotting analysis | 第43-44页 |
| 3.6 Overexpression of GhMYC2-like activate JA signal pathway in cotton | 第44页 |
| 3.7 Overexpression of GhMYC2-like makes the plants more tolerant to insect feeding | 第44-46页 |
| 3.8 Virus induced gene silencing studies in Gossypium barbedense | 第46-49页 |
| 3.8.1 Inoculation and confirmation of GhMYC2-like silencing through RT-PCR | 第46页 |
| 3.8.2 Silencing of GhMYC2-like through VIGS enhances cotton susceptible to Verticillium dahliae | 第46-48页 |
| 3.8.3 MYC2-like silencing leads cotton more susceptible to B. cinerea | 第48-49页 |
| 4. DISCUSSION | 第49-53页 |
| 5. Major findings and future recommendations | 第53-55页 |
| 5.1 Major Findings | 第53-54页 |
| 5.2 Future Recommendations | 第54-55页 |
| REFERENCES | 第55-68页 |
| DEDICATION | 第68-69页 |
| ACKNOWLEDGEMENT | 第69-70页 |
