| 摘要 | 第5-7页 |
| ABSTRACT | 第7-8页 |
| CHAPTER ONE: INTRODUCTION | 第12-34页 |
| 1.1 Background of the study | 第12-13页 |
| 1.2 Literature review | 第13-31页 |
| 1.2.1 Importance of the Potato | 第13-14页 |
| 1.2.2 Pectolytic bacterial pathogens of potatoes | 第14-23页 |
| 1.2.3 Bacteriophages | 第23-31页 |
| 1.3 Problem statement | 第31-32页 |
| 1.4 Justification | 第32-33页 |
| 1.5 Objectives | 第33页 |
| 1.6 Hypotheses | 第33-34页 |
| CHAPTER TWO: ISOLATION AND CHARACTERIZATION OF PECTOLYTIC BACTERIAL PATHOGENS INFECTING POTATOES | 第34-55页 |
| 2.1 Materials and methods | 第34-37页 |
| 2.1.1 Bacterial isolation and culture conditions | 第34-35页 |
| 2.1.2 Pathogenicity tests | 第35页 |
| 2.1.3 Pectolytic activity tests | 第35页 |
| 2.1.4 Ribosomal DNA (16S rDNA) sequencing and Y1/Y2 primers DNA amplification | 第35-36页 |
| 2.1.5 Identification of the bacterial strains using BIOLOG microbial identification system (BIOLOG) | 第36-37页 |
| 2.1.6 Phylogenetic Analysis | 第37页 |
| 2.2 Results | 第37-52页 |
| 2.2.1 Bacteria sample isolation | 第37-38页 |
| 2.2.2 16S rDNA Sequencing and Y1/Y2 primers DNA amplification | 第38-39页 |
| 2.2.3 BIOLOG microbial identification | 第39-48页 |
| 2.2.4 Phylogenetic analysis | 第48-51页 |
| 2.2.5 Pathogenicity test results | 第51页 |
| 2.2.6 Pectolytic test results | 第51-52页 |
| 2.3 Discussion | 第52-55页 |
| 2.3.1 Phytopathogenic pectolytic bacteria infecting potatoes in Nakuru county, Kenya | 第52-53页 |
| 2.3.2 Pectolytic activity of isolated bacteria at different incubation temperatures | 第53-55页 |
| CHAPTER THREE: ISOLATION, CHARACTERIZATION AND USE OF BACTERIOPHAGES TO CONTROL PECTOBACTERIUM CAROTOVORUM | 第55-70页 |
| 3.1 Materials and Methods | 第55-59页 |
| 3.1.1 Phage isolation and purification | 第55-56页 |
| 3.1.2 Phage amplification | 第56页 |
| 3.1.3 Morphology of host bacteria | 第56页 |
| 3.1.4 Phage morphology assessment by transmission electron microscope | 第56-57页 |
| 3.1.5 Lytic activity of phages against Pectobacterium carotovorum | 第57页 |
| 3.1.6 Bacteriophage one step growth curve | 第57页 |
| 3.1.7 Antibacterial activity and phage specificity | 第57-58页 |
| 3.1.8 Development of phage resistance in Pectobacterium carotovorum | 第58页 |
| 3.1.9 Bacteriophage efficacy tests in controlling potato soft rot | 第58-59页 |
| 3.2 Results | 第59-67页 |
| 3.2.1 Phage isolation and purification | 第59-60页 |
| 3.2.2 Phhage morphoology | 第60-62页 |
| 3.2.3 Lyytic activityy of phagess against Peectobacterium carotov vorum | 第62-63页 |
| 3.2.4 Bacteriophage one step growth curve | 第63-64页 |
| 3.2.5 Antibacterial activity and phage specificity | 第64页 |
| 3.2.6 Development of resistance against bacteriophage infection | 第64-65页 |
| 3.2.7 Bacteriophage efficacy tests in controlling potato soft rot | 第65-67页 |
| 3.3 Disccussion | 第67-70页 |
| 3.3.1 Isolated bacteriophages for the control of Pectobacterium carotovorum | 第67-68页 |
| 3.3.2 Bacteriophage cocktail to overcome phage-resistance in Pectobacterium carotovorum | 第68-70页 |
| CHAPTER FOUR: CONCLUSION AND RECOMMENDATIONS | 第70-73页 |
| 4.1 Conclusion | 第70-71页 |
| 4.2 Recommendations and future prospects | 第71-73页 |
| REFERENCES | 第73-81页 |
| LIST OF ABBREVIATIONS AND ACRONYMS | 第81-82页 |
| APPENDICES | 第82-85页 |
| ACKNOWLEDGEMENTS | 第85-86页 |
| RéSUMé and PUBLISHED PAPERS | 第86-87页 |
