| Introduction | 第10-13页 |
| Literature review | 第13-38页 |
| 1 Metabolomics of adverse pregnancy outcomes | 第15-28页 |
| 1.1 Preeclampsia | 第15-20页 |
| 1.2 Fetal Growth Restriction | 第20-23页 |
| 1.3 Preterm Birth | 第23-25页 |
| 1.4 Gestational Diabetes Mellitus | 第25-28页 |
| 2 Hair Metabolomics | 第28-36页 |
| 2.1 Background, Structure, and Growth of Hair | 第28-29页 |
| 2.2 The hair follicle growth in 3 distinct phases; anagen, catagen and telogen phase | 第29-30页 |
| 2.3 General use of Hair in clinical applications | 第30-34页 |
| 2.3.1 Trace elements exposure | 第30-31页 |
| 2.3.2 Drugs abuse analysis | 第31-32页 |
| 2.3.3 Hair analysis related to smoking of tobacco | 第32-33页 |
| 2.3.4 Alcohol consumption via hair analysis | 第33-34页 |
| 2.3.5 Pesticides exposure via hair analysis | 第34页 |
| 2.4 Hair metabolome related to adverse pregnancy outcomes | 第34-36页 |
| 3 Relating exposome to adverse pregnancy outcomes | 第36-37页 |
| 4 Prospective/Future trends | 第37-38页 |
| Methods | 第38-58页 |
| 5 Hair sample collection | 第39-40页 |
| 6 Sample preparation procedures | 第40-46页 |
| 6.1 Segmentation of hair sample | 第40-41页 |
| 6.2 Basic hydrolysis of the hair sample | 第41-42页 |
| 6.3 Liquid-liquid extraction | 第42-44页 |
| 6.4 Methyl chloroformate derivatization | 第44-46页 |
| 7 Analysis procedures | 第46-47页 |
| 7.1 Gas chromatography-mass spectrometry | 第46页 |
| 7.2 Liquid chromatography-mass spectrometry | 第46-47页 |
| 8 Data Extraction and Clean-Up | 第47-55页 |
| 8.1 Gas chromatography-mass spectrometry | 第47-54页 |
| 8.1.1 Data Extraction by AMDIS | 第47-48页 |
| 8.1.1.1 In-house MCF library | 第47-48页 |
| 8.1.1.2 NIST14 library | 第48页 |
| 8.1.2 Clean-Up procedure | 第48-54页 |
| 8.1.2.1 First adjustments summary report | 第48-49页 |
| 8.1.2.2 Contaminants check | 第49-50页 |
| 8.1.2.3 Relative abundance profile check | 第50-51页 |
| 8.1.2.4 Retention time check | 第51-52页 |
| 8.1.2.5 Noise check | 第52-54页 |
| 8.1.2.6 Peak reintegration | 第54页 |
| 8.2 Liquid chromatography-mass spectrometry | 第54-55页 |
| 8.2.1 Data extraction by Progenesis | 第54-55页 |
| 9 Data analysis and Statistics | 第55-58页 |
| 9.1 Normalization for gas chromatography-mass spectrometry data | 第55页 |
| 9.1.1 Auckland study | 第55页 |
| 9.1.2 GUSTO study | 第55页 |
| 9.2 Statistics for gas chromatography-mass spectrometry data | 第55-56页 |
| 9.2.1 Auckland study | 第55-56页 |
| 9.2.2 GUSTO study | 第56页 |
| 9.3 Normalization for liquid chromatography-mass spectrometry data | 第56页 |
| 9.3.1 Auckland Study | 第56页 |
| 9.3.2 GUSTO study | 第56页 |
| 9.4 Statistics for liquid chromatography-mass spectrometry data | 第56-58页 |
| 9.4.1 Auckland study | 第56-57页 |
| 9.4.2 GUSTO study | 第57-58页 |
| Results and Discussion | 第58-96页 |
| 10 Auckland segmental study | 第59-82页 |
| 10.1 Aim of study | 第59页 |
| 10.2 Study design | 第59-60页 |
| 10.3 Results and Discussion | 第60-82页 |
| 10.3.1 Gas Chromatography-mass spectrometry | 第60-71页 |
| 10.3.1.1 Results | 第60-68页 |
| 10.3.1.1.1 Longitudinal analysis of hair metabolome for each trimester of normal pregnancy | 第64-67页 |
| 10.3.1.1.2 Comparison of healthy pregnancies and complicated pregnancies | 第67-68页 |
| 10.3.1.2 Discussion | 第68-71页 |
| 10.3.1.2.1 Changes in the hair metabolome through the trimesters, in healthy pregnancy | 第69-70页 |
| 10.3.1.2.2 Hair metabolome of pregnancy complications | 第70-71页 |
| 10.3.2 Liquid chromatography-mass spectrometry | 第71-82页 |
| 10.3.2.1 Results | 第71-80页 |
| 10.3.2.1.1 Longitudinal analysis of hair metabolome for each trimester of normal pregnancy | 第72页 |
| 10.3.2.1.2 Comparison of healthy pregnancies and complicated pregnancies | 第72-80页 |
| 10.3.2.2 Discussion | 第80-82页 |
| 10.3.2.2.1 Changes in the hair metabolome through the trimesters, in healthy pregnancy | 第80页 |
| 10.3.2.2.2 Hair metabolome of pregnancy complications | 第80-82页 |
| 11 Gusto Hair exposome Study | 第82-96页 |
| 11.1 Aim of Study | 第82页 |
| 11.2 Philosophy behind GUSTO | 第82-83页 |
| 11.3 Study design | 第83-84页 |
| 11.4 Results and Discussion | 第84-92页 |
| 11.5 Discussion | 第92-96页 |
| 11.5.1 Comparison of healthy pregnancies and complicated pregnancies | 第92-93页 |
| 11.5.2 Demographic and clinical data logistic regression against mid pregnancy hair metabolome | 第93-96页 |
| Conclusion | 第96-99页 |
| Acknowledgements | 第99-101页 |
| References | 第101-120页 |
