| Table of content | 第4-7页 |
| Abstract | 第7-8页 |
| List of Abbreviations | 第9-10页 |
| Chapter Ⅰ Preface | 第10-24页 |
| 1. Introduction | 第10页 |
| 2. Properties of QDs | 第10-15页 |
| 2.1 The Optical Properties of QDs | 第11-13页 |
| 2.1.1 Light Absorbance | 第11-12页 |
| 2.1.2 Emission | 第12-13页 |
| 2.2 Physical Properties | 第13-15页 |
| 2.2.1 Structure | 第13-14页 |
| 2.2.2 Size | 第14-15页 |
| 2.3 Material | 第15页 |
| 3 Coating of QDs and surface modification | 第15-19页 |
| 3.1 Surface shielding chemistries | 第16页 |
| 3.2 Exchange of surface hydrophobic ligands | 第16-17页 |
| 3.3 The goal of surface chemistries | 第17页 |
| 3.4 The functions of the attachment point of QDs | 第17-19页 |
| 3.4.1 Advantages of multiple functions combination on QDs surfaces | 第18页 |
| 3.4.2 Disadvantages of multiple functions combination on QDs surfaces | 第18-19页 |
| 4 Fluorescence as a new tool for of biological sample imaging | 第19页 |
| 5 The suitability of quantum dots for probing goals | 第19-20页 |
| 6 Photostability and fluorescence of QDs compared with dyes | 第20-21页 |
| 6.1 Comparison of physical properties | 第20-21页 |
| 6.2 Comparison of the spectra | 第21页 |
| 7 Fluorescence resonance energy transfer | 第21-22页 |
| 7.1 Advantage of FRET measurements: | 第21页 |
| 7.2 Disadvantage of FRET measurements | 第21-22页 |
| 7.3 Influence of these disadvantage on QDs photophysical properties | 第22页 |
| 8 Time gated and lifetime imaging of samples labeled with QDs | 第22-23页 |
| 9 Conclusion | 第23-24页 |
| The design and significance of the research issues | 第24-25页 |
| Chapter Ⅱ:study of the interaction between eggs albumin(OVA)and CdTe QDs | 第25-34页 |
| 1. Introduction | 第25-26页 |
| 2 Material and methods | 第26-27页 |
| 2.1 Preparation of buffer solution | 第26页 |
| 2.2 Preparation of the CdTe QDs | 第26页 |
| 2.3 Preparation of OVA solution | 第26-27页 |
| 3 Interaction study between OVA and CdTe QDs | 第27-29页 |
| 3.1 Optimization of the concentration for the as preparing CdTe QDs | 第27-28页 |
| 3.2 Optimization of the reaction pH | 第28页 |
| 3.3 Optimization of the reaction time | 第28-29页 |
| 4 Interaction study between CdTe QDs and OVA | 第29-30页 |
| 5 Effect of OVA concentration on the fluorescence of QDs | 第30-31页 |
| 6 Effect of temperature on the quenching process | 第31-32页 |
| 7 conclusion | 第32-34页 |
| Chapter Ⅲ:CdTe QDs for the detection of Hg~(2+) ions | 第34-52页 |
| 1 Introduction | 第34-36页 |
| 2 Material and methods | 第36页 |
| 2.1 Apparatus | 第36页 |
| 2.1.1 Material for the measurement of UV-Vis absorption spectra | 第36页 |
| 2.1.2 Material for the measurement of FL and RLS spectra | 第36页 |
| 3 Chemicals | 第36-37页 |
| 4 Synthesis and purification of CdTe QDs | 第37-39页 |
| 4.1 Preparation of GSH capped CdTe Qdots | 第37页 |
| 4.1.2 Purification of CdTe QDs | 第37页 |
| 4.2 Characterization of CdTe QDs | 第37-39页 |
| 4.2.1 UV-Vis and fluorescence spectra | 第37-39页 |
| 5 Optimization of the reaction's conditions | 第39-41页 |
| 5.1 Optimization of the pH of the buffer solution | 第39-41页 |
| 5.1.1 The buffer preparation | 第39-40页 |
| 5.1.2 Optimization of the reaction time | 第40页 |
| 5.1.3 Optimization of the concentration of QDs | 第40-41页 |
| 6 Effect of different metal ions | 第41-42页 |
| 7 The quenching effect of mercury ions | 第42-43页 |
| 8 Calibration curve and detection limit | 第43-44页 |
| 9 Study of the interaction between CdTe QDs and Hg~(2+),the study scope | 第44-49页 |
| 9.1 Interpretation of the UV-Vis spectra | 第44-45页 |
| 9.2 Effect of Hg~(2+) ions on the RLS spectra | 第45-47页 |
| 9.3 Interpretation of the mechanism of interaction between quantum dots and Hg~(2+) Ions | 第47-48页 |
| 9.4 High through put method scans for thermodynamic study | 第48-49页 |
| 10 Analytical application | 第49-51页 |
| 11 Conclusion | 第51-52页 |
| General conclusion | 第52-53页 |
| References | 第53-59页 |
| Acknowledgements | 第59页 |
