| Abstract | 第6页 |
| CHAPTER -Ⅰ Cloning and identification of low nitrogen stress induced genes in rice (Oryza sativa L). | 第7-28页 |
| 1.1 Literature Review | 第7-16页 |
| 1.1.1 Introduction | 第7-8页 |
| 1.1.2 Nitrate assimilation | 第8-11页 |
| 1.1.3 Ammonium uptake and transporter | 第11-12页 |
| 1.1.4 Genes involved in ammonium assimilation | 第12-16页 |
| 1.2 Objectives and Significance of the Proposed Research | 第16-17页 |
| 1.2.1 Objective | 第16页 |
| 1.2.2 Significance | 第16-17页 |
| 1.3 Materials and Methods | 第17-19页 |
| 1.3.1 Methods and approaches used in this study | 第17页 |
| 1.3.2 Construction vector and transformation | 第17-19页 |
| 1.3.3 Northern and Southern bolt analysis | 第19页 |
| 1.3.4 Plant growth conditions | 第19页 |
| 1.4 Results | 第19-26页 |
| 1.4.1 Selecting the candidate genes | 第19-20页 |
| 1.4.2 Overexpression of the candidate genes | 第20-24页 |
| 1.4.3 Silencing the candidate genes | 第24页 |
| 1.4.4 Promoter analysis of the candidate genes | 第24-26页 |
| 1.5 Discussion | 第26-28页 |
| 1.5.1 Possible reasons for the failure of the first study | 第26-28页 |
| CHAPTER - Ⅱ Sequence and expression analysis of the thioredoxin protein gene family in rice | 第28-70页 |
| 2.1 Literature Review | 第28-36页 |
| 2.1.1 Introduction | 第28-30页 |
| 2.1.2 Different types of disulfide groups | 第30页 |
| 2.1.3 Function of disulfides | 第30-31页 |
| 2.1.4 Reversible activation/deactivation of enzymes in response to light/dark conditions | 第31-32页 |
| 2.1.5 Mitochondrial processes | 第32-33页 |
| 2.1.6 Regulation linked to NADP | 第33页 |
| 2.1.7 Information about Trx gene in different organism and metabolism | 第33-36页 |
| 2.2 Objectives and Significance of the Proposed Research | 第36页 |
| 2.2.1 Objectives of the Study | 第36页 |
| 2.2.2 Singnificance | 第36页 |
| 2.3 Materials and Methods | 第36-41页 |
| 2.3.1 Database searches | 第36-37页 |
| 2.3.2 Sequence analysis | 第37页 |
| 2.3.3 Plant material and growth conditions | 第37页 |
| 2.3.4 Expression profile analysis | 第37-41页 |
| 2.4 Results | 第41-66页 |
| 2.4.1 Identification of Trx genes in the rice genome | 第41-44页 |
| 2.4.2 Protein sequence and phylogenetic analysis of the Trx family | 第44-51页 |
| 2.4.3 Expression profiles of Trx genes in different tissues | 第51-59页 |
| 2.4.4 Expression of Trx genes under hormone stress conditions | 第59-64页 |
| 2.4.5 Expression of OsTrx genes under light and dark treatment at the plumule and radicle stages | 第64-66页 |
| 2.5 Discussion | 第66-69页 |
| 2.5.1 Expression profiles of Trx genes in different tissues | 第67-68页 |
| 2.5.2 Expression of Trx genes under various treatment conditions | 第68-69页 |
| 2.6 Conclusions and Further Study | 第69-70页 |
| References | 第70-82页 |
| Protocols | 第82-93页 |
| Curriculum Vitae | 第93-94页 |
| Publication | 第94-95页 |
| Acknowledgement | 第95页 |
