| ABSTRACT | 第8-12页 |
| 摘要 | 第13-17页 |
| LIST OF ABBREVIATIONS | 第17-19页 |
| PART 1 LITERATURE REVIEW | 第19-49页 |
| Chapter 1 An overview of cotton leaf curl disease | 第21-29页 |
| 1.1 Cotton culy leaf disease and geminiviruses | 第21页 |
| 1.2 Characteristics of Geminiviridae | 第21-22页 |
| 1.3 Classification of Geminiviridae | 第22-24页 |
| 1.3.1 Mastrevirus | 第22页 |
| 1.3.2 Curtovirus | 第22-23页 |
| 1.3.3 Topocuvirus | 第23页 |
| 1.3.4 Begomovirus | 第23-24页 |
| 1.4 Origin of disease and its impacts on production | 第24-29页 |
| 1.4.1 CLCuD and yield losses | 第25-26页 |
| 1.4.2 Signs and symptoms | 第26-27页 |
| 1.4.3 The host range of CLCuD | 第27-29页 |
| Chapter 2 Valuable resistant resources in diploid cotton species | 第29-33页 |
| Chapter 3 Strategies to manage CLCuD | 第33-47页 |
| 3.1 Molecular breeding for disease | 第33-34页 |
| 3.2 Breeding for virus resistance | 第34页 |
| 3.3 Employment of molecular markers | 第34-36页 |
| 3.3.1 Microsatellites(SSR) | 第35-36页 |
| 3.4 Marker assisted selection(MAS) | 第36-39页 |
| 3.5 Quantitative trait loci mapping of disease resistance | 第39-47页 |
| 3.5.1 Development of mapping population | 第39-43页 |
| 3.5.2 Bulk segregant analysis | 第43-44页 |
| 3.5.3 Statistical approaches for QTL identification | 第44-47页 |
| Over all objective of study | 第47-49页 |
| PART Ⅱ ACADEMIC REPORT | 第49-155页 |
| Chapter 4 Transferrence of resistances to cotton leaf curl disease from Gossypium arboreum into G. hirsutum via interspecific hybridization | 第49-83页 |
| 4.1 Introduction | 第49-51页 |
| 4.2 Materials and methods | 第51-57页 |
| 4.2.1 Plant materials | 第51页 |
| 4.2.2 Development of backcross progenies | 第51-53页 |
| 4.2.3 Cross fertility studies | 第53页 |
| 4.2.4 Morphological characteristics | 第53页 |
| 4.2.5 Cytological studies | 第53-54页 |
| 4.2.6 Maintenance of virus inoculum and screening for CLCuD | 第54页 |
| 4.2.7 Phenotypic assessment of BC_1～BC_3 progenies against CLCuD | 第54-55页 |
| 4.2.8 Inoculation of CLCuD through grafting | 第55-57页 |
| 4.3 Results | 第57-77页 |
| 4.3.1 Development of backcross populations from an interspecific cross between G.arboreum and G. hirsutum | 第57-70页 |
| 4.3.2 Evaluation of cotton leaf curl virus resistance in backcross populations between G.arboreum and G.hirsutum | 第70-77页 |
| 4.4 Discussion | 第77-81页 |
| 4.5 Conclusions | 第81-83页 |
| Chapter 5 Association analysis between SSR markers and traits from G. arboreum in G.hirsutum | 第83-139页 |
| 5.1 Introduction | 第83-86页 |
| 5.2 Materials and methods | 第86-102页 |
| 5.2.1 Plant materials | 第86页 |
| 5.2.2 Molecular marker evaluation | 第86-89页 |
| 5.2.3 Simple sequence repeats (SSRs) evaluation | 第89-90页 |
| 5.2.4 PCR reaction mixture and conditions for SSR amplification | 第90-96页 |
| 5.2.5 Scoring of SSR amplified bands and genotyping | 第96页 |
| 5.2.6 Identification of donor chromosome segments from G. arboreum in G. hirsutum via SSR markers | 第96-97页 |
| 5.2.7 Association analysis between traits and molecular markers | 第97页 |
| 5.2.8 QTL analysis | 第97-98页 |
| 5.2.9 Observations and measurements for phenotypic trials | 第98-100页 |
| 5.2.10 Pearson correlation coefficient (r) | 第100-101页 |
| 5.2.11 Principal component analysis | 第101-102页 |
| 5.3 Results | 第102-126页 |
| 5.3.1 Phenological variation in BC_3～BC_4 populations from an interspecific cross between Gossypium arboreum and Gossypium hirsutum | 第102-109页 |
| 5.3.2 Screening through SSRs primers | 第109-111页 |
| 5.3.3 Identification of donor chromosome segments from G. arboreum in G. hirsutum via SSR markers | 第111-114页 |
| 5.3.4 Association analysis between traits and molecular markers | 第114-119页 |
| 5.3.5 Quantitative traits loci | 第119-126页 |
| 5.4 Discussion | 第126-136页 |
| 5.4.1 Phenological variation in BC_3-BC_4 populations from an interspecific cross between Gossypium arboreum and Gossypium hirsutum | 第126页 |
| 5.4.2 Molecular breeding | 第126-127页 |
| 5.4.3 Choice of mapping population and size | 第127-128页 |
| 5.4.4 Parental screening and choice of DNA markers | 第128-129页 |
| 5.4.5 Identification of donor chromosome segments from G. arboreum in G. hirsutum | 第129-130页 |
| 5.4.6 Association analysis between traits and molecular markers | 第130页 |
| 5.4.7 QTL mapping | 第130-136页 |
| 5.5 Conclusions | 第136-139页 |
| Chapter 6 Transferrence of resistances to cotton leaf curl disease and increased fibre strength from Gossypium stocksii into G. hirsutum via interspecific hybridization | 第139-153页 |
| 6.1 Introduction | 第139-141页 |
| 6.2 Materials and methods | 第141-144页 |
| 6.2.1 Colchicine treatment | 第141页 |
| 6.2.2 Morphological characteristics | 第141页 |
| 6.2.3 Pollen viability estimation | 第141-142页 |
| 6.2.4 Cytological studies: | 第142页 |
| 6.2.5 Evaluation of F_1 hybrid plants resisitant to CLCuD | 第142页 |
| 6.2.6 Measurement of fiber qualities | 第142-144页 |
| 6.3 Results | 第144-151页 |
| 6.3.1 Development of F_1 hexaploid from an interspecific cross between Gossypium hirsutum and Gossypium stocksii | 第144-150页 |
| 6.3.2 Evaluation of resistance to CLCuD and increased fibre strength in F_1 hexaploid from an interspecific cross between G. hirsutum and G. stocksii | 第150-151页 |
| 6.4 Discussion | 第151-152页 |
| 6.5 Conclusions | 第152-153页 |
| Over all conclusions of the study | 第153-155页 |
| Innovations of the dissertation | 第155-157页 |
| List of publications | 第157-159页 |
| References | 第159-179页 |
| Appendix | 第179-199页 |
| Acknowledgement | 第199-200页 |
