| 1 Brief review of microsatellites and single nucleotide polymorphism derived from expressed sequence tags | 第1-15页 |
| ·What are Expressed Sequence Tags(ESTs)? | 第6-7页 |
| ·What are microsatellites? | 第7-10页 |
| ·How to mine SSRs from ESTs? | 第7-8页 |
| SSR detection tools available | 第7页 |
| Protocols for mining SSRs from ESTs | 第7-8页 |
| Annotation of SSR-ESTs | 第8页 |
| ·Mini-review of some reports on EST-derived SSRs in plants | 第8-10页 |
| Frequencies, types and distributions of EST-SSRs | 第8-9页 |
| EST-SSRs in genetic mapping | 第9页 |
| Function of the genes that contain SSR motifs | 第9页 |
| Transferability of EST-SSRs among species | 第9-10页 |
| Other genetic studies | 第10页 |
| ·What are single nueleotide polymorphisms(SNPs)? | 第10-13页 |
| ·SNP discovery and validation | 第10-11页 |
| ·Distribution and frequency of SNPs | 第11-12页 |
| ·SNP and Haplotype structure and linkage disequilibium(LD) | 第12-13页 |
| ·How to discover SNPs in bread wheat | 第13页 |
| ·The target of this study | 第13-15页 |
| 2 Analysis of microsatellites in major crops assessed by computational and experimental approaches | 第15-34页 |
| ·Abstract | 第15页 |
| ·Introduction | 第15-16页 |
| ·Materials and Methods | 第16-17页 |
| Sources of EST sequences | 第16页 |
| Mining SSRs from ESTs | 第16-17页 |
| Development of EST-SSR markers from wheat EST sequences and evaluation of their usefulness in wheat and other species | 第17页 |
| ·Results and discussion | 第17-34页 |
| Evaluation of frequencies of EST-SSRs in different crop species and prediction of their functions | 第17-18页 |
| EST-SSR marker development and evaluation | 第18-19页 |
| Conservation of EST-SSR sequences acrosss pecies | 第19-34页 |
| 3 101New Microsatellite Loci Derived from ESTs(EST-SSRs) in Bread Wheat | 第34-47页 |
| ·Abstract | 第34页 |
| ·Introduction | 第34-35页 |
| ·Materials and methods | 第35-36页 |
| Microsatellite marker development | 第35页 |
| Plant materials, DNA extraction and PCR amplification | 第35页 |
| Genetic mapping | 第35页 |
| Homology searching | 第35-36页 |
| ·Results | 第36页 |
| Marker evaluation:functionality and polymorphism | 第36页 |
| Mapping and distribution of microsatellite loci | 第36页 |
| Putative genic functions of the mapped genes | 第36页 |
| ·Discussion | 第36-47页 |
| 4 Validation of single nucleotide polymorphism(SNPs) from wheat ESTs | 第47-51页 |
| ·Plant materials | 第47页 |
| ·Protocols for SNP validation | 第47-49页 |
| ·Results and Discussions | 第49-51页 |
| References | 第51-56页 |
| Acknowledgements | 第56-57页 |
