| Table of Contents | 第1-6页 |
| Abstract | 第6-9页 |
| 中文摘要 | 第9-12页 |
| Abbreviations / Acronyms | 第12-13页 |
| Chapter Ⅰ:A Brief review about MLCK and Cell Migration | 第13-37页 |
| ·Biology of MLCK | 第14-21页 |
| ·Vertebrate MLCK family | 第15-16页 |
| ·Structure of smooth muscle MLCK | 第16-17页 |
| ·Function of MLCK in smooth muscle | 第17-18页 |
| ·Function of MLCK in nonmuscle cells | 第18-19页 |
| ·Non-kinase activity of MLCK | 第19-21页 |
| ·Cell migration | 第21-29页 |
| ·Membrane protrusion | 第23-25页 |
| ·Nonmuscle myosin Ⅱ | 第25-27页 |
| ·Membrane tension | 第27-29页 |
| References | 第29-37页 |
| Chapter Ⅱ:Absence of MLCK Accelerates Smooth Muscle Cell Migration, Protrusion and Spreading by Reducing Cell Membrane Tension,through a RLC Phosphorylation independent way | 第37-78页 |
| Introduction | 第38-40页 |
| Materials and methods | 第40-45页 |
| Results | 第45-69页 |
| Section ⅠCharacterization of MLCK-deficient smooth muscle cells | 第45-49页 |
| ·Establishment of primary cultured MLCK-deficient and control SMCs | 第45-46页 |
| ·MLCK expression pattern in primary cultured intestinal SMCs | 第46-48页 |
| ·MLCK-deficient SMCs showed normal cell proliferation | 第48-49页 |
| Section Ⅱ Absence of MLCK promoted SMCs migration | 第49-52页 |
| ·MLCK-deficient SMCs migrated out from cultural explants faster | 第49-50页 |
| ·Absence of MLCK promoted SMCs migration in wound healing | 第50-52页 |
| Section Ⅲ Membrane protrusion and cell spreading were enhanced in MLCK-deficient SMCs | 第52-56页 |
| ·MLCK-deficient SMCs showed enhanced membrane protrusion | 第52-53页 |
| ·MLCK-deficient SMCs showed enhanced cell spreading | 第53-55页 |
| ·Full-length MLCK re-expression could rescue the accelerated spreading phenotype | 第55-56页 |
| Section Ⅳ MLCK controlled motility and tension of the cell membrane | 第56-60页 |
| ·MLCK-deficient SMCs showed higher membrane motility | 第56-58页 |
| ·MLCK-deficient SMCs showed reduced membrane tension | 第58-60页 |
| Section Ⅴ MLCK regulated cortical F-actin cytoskeleton | 第60-64页 |
| ·MLCK-deficient SMCs displayed loose cortical F-actin cytoskeleton | 第60-62页 |
| ·Reduction of F-actin stabilization was necessary for membrane protrusion and cell spreading | 第62-64页 |
| Section Ⅵ Myosin regulatory light chain phosphorylation was not affected in MLCK-deficient SMCs | 第64-66页 |
| ·MLCK-deficient SMCs showed no significant change in RLC phosphorylation | 第64-66页 |
| Section Ⅶ MLCK involved in fibronectin-integrin-cytoskeleton complex | 第66-69页 |
| ·MLCK could be found in the protein complexes pulled down by fibronectin | 第66-68页 |
| ·A working model of MLCK regulating protrusion and cell migration by controlling membrane tension | 第68-69页 |
| ·On going work | 第69页 |
| Conclusion and Discussion | 第69-72页 |
| References | 第72-78页 |
| Acknowledgements | 第78-79页 |
| Publications | 第79-81页 |
