| 论文摘要 | 第1-8页 |
| ABSTRACT | 第8-10页 |
| 致谢 | 第10-12页 |
| REMERCIEMENTS | 第12-15页 |
| TABLE AND MATERIALS | 第15-17页 |
| TABLE OF FIGURES | 第17-18页 |
| ABREVIATIONS | 第18-21页 |
| 摘要 | 第21-29页 |
| PREFACE | 第29-33页 |
| INTRODUCTION_ON FLUORESCENCE | 第33-75页 |
| Ⅰ.1 General Definitions:Characteristics of fluorescence and the fluorescence microscope | 第35-50页 |
| Ⅰ.1.1 Photoluminescence process | 第35-38页 |
| Ⅰ.1.2 Steady-state characteristics of fluorescence emission | 第38-48页 |
| Ⅰ.1.3 Time-resolved characteristics of fluorescence emission,fuorescence lifetime | 第48-50页 |
| Ⅰ.2 Fluorescence anisotropy and instrumental considerations | 第50-59页 |
| Ⅰ.2.1 Steady-state fluorescence anisotropy:definition andprinciple | 第50-53页 |
| Ⅰ.2.2 Time-resolved fluorescence anisotropy:definition and principle | 第53-54页 |
| Ⅰ.2.3 Instrumentation and design of pulse fluorometers | 第54-57页 |
| Ⅰ.2.4 Examples of application of fluorescence anisotropy in the field of biology | 第57-59页 |
| Ⅰ.3 Fluorescence correlation spectroscopy | 第59-75页 |
| Ⅰ.3.1 Fluorescence correlation spectroscopy:definition | 第60-63页 |
| Ⅰ.3.2 Conceptual basis of Fluorescence correlation spectroscopy and determination of diffusion constant | 第63-64页 |
| Ⅰ.3.3 Fluorescence cross-correlation spectroscopy:definition and conceptual basis | 第64-66页 |
| Ⅰ.3.4 Instrumentations and methods of FCS and FCCS | 第66-72页 |
| Ⅰ.3.5 Example of application of FCS and FCCS in the field of biology | 第72-75页 |
| MOTIVATION AND OBJECTIVE | 第75-78页 |
| MATERIAL AND METHODES | 第78-89页 |
| Ⅱ.1 Reagents and buffers | 第79-81页 |
| Ⅱ.1.1 HIV integrase related buffers | 第79-80页 |
| Ⅱ.1.2 RecQ helicase related buffers | 第80-81页 |
| Ⅱ.2 Protein and oligonucleotides purification | 第81-84页 |
| Ⅱ.2.1 HIV integrase purification | 第81-82页 |
| Ⅱ.2.2 RecQ helicase purification | 第82-83页 |
| Ⅱ.2.3 Oligonucleotides purification and preparation | 第83-84页 |
| Ⅱ.3 Enzymatic activity measurements | 第84-89页 |
| Ⅱ.3.1 Steady-state fluorescence based activity measurements | 第84-85页 |
| Ⅱ.3.2 Fluorescence cross-correlation measurements | 第85-88页 |
| Ⅱ.3.3 Quantified maximum entropy method of time-resolved fluorescence data(MEM) | 第88-89页 |
| APPLICATION OF FCCS TO STUDY THE ENZYMATIC KINETICS OF RECQ HELICASE | 第89-149页 |
| Ⅲ.1 General introduction to the relationship between DNA and helicases | 第90-102页 |
| Ⅲ.1.1 Structure and function of DNA | 第90-92页 |
| Ⅲ.1.2 The function of helicase in cellular processes involved with DNA | 第92-95页 |
| Ⅲ.1.3 Helicase characteristics and helicase deficiency diseases | 第95-102页 |
| Ⅲ.2 E.Coli RecQ Helicase Unwinding Activity as Monitored by Dual Color Fluorescence Correlation Spectroscopy | 第102-131页 |
| Ⅲ.2.1 Introduction | 第102-103页 |
| Ⅲ.2.2 Manuscript | 第103-128页 |
| Ⅲ.2.3 Conclusion | 第128-131页 |
| Ⅲ.3 Human RecQ5β Unwinding and Strand-Annealing Activity as Monitored by Dual Color Fluorescence Correlation Spectroscopy | 第131-149页 |
| Ⅲ.3.1 INTRODUCTION | 第131-132页 |
| Ⅲ.3.2 MATERIALS AND METHODS | 第132-133页 |
| Ⅲ.3.3 RESULTS | 第133-137页 |
| Ⅲ.3.4 DISCUSSION | 第137-140页 |
| Ⅲ.3.5 PROSPECTIVE | 第140-141页 |
| Ⅲ.3.6 FIG URES AND LEGENDS | 第141-149页 |
| APPLICATION OF FLUORESCENCE ANISOTROPY TO STUDY ENZYMATIC KINETICS OF HIV-IN | 第149-177页 |
| Ⅳ.1 The basic biology of retroviruses | 第150-164页 |
| Ⅳ.1.1 Structural taxonomic for the family of retroviruses | 第150-152页 |
| Ⅳ.1.2 Replication cycle and pathology of HIV-virus | 第152-155页 |
| Ⅳ.1.3 Introduction to anti-retroviral treatments | 第155-158页 |
| Ⅳ.1.4 Structure and function of HIV-integrase | 第158-164页 |
| Ⅳ.2 The G140S mutation in HIV integrases from raltegravir-resistant patients rescues catalytic defect due to the resistance Q148H mutation | 第164-177页 |
| Ⅳ.2.1 Introduction | 第164-165页 |
| Ⅳ.2.2 Manuscript | 第165-175页 |
| Ⅳ.2.3 Conclusion | 第175-177页 |
| APPLICATION OF PHOTOLUMINESCENCE DECAY TO CHRACTERIZE QDS FLUORESCENCE PROPERTIES | 第177-208页 |
| Ⅴ.1 General introduction to the semiconductor nanoparticles | 第178-189页 |
| Ⅴ.1.1 Definition of quantum dots | 第178-181页 |
| Ⅴ.1.2 Spectral properties of quantum dots | 第181-182页 |
| Ⅴ.1.3 Solubilization and biological applications of quantum dots | 第182-189页 |
| Ⅴ.2 Multi-exponential Photoluminescence Decay of CdTe Quantum Dots upon One and Two Photon Excitation | 第189-208页 |
| Ⅴ.2.1 INTRODUCTION | 第189-190页 |
| Ⅴ.2.2 MATERIALS AND METHODS | 第190-194页 |
| Ⅴ.2.3 RESULTS | 第194-197页 |
| Ⅴ.2.4 DISCUSSION AND CONCLUSION | 第197-199页 |
| Ⅴ.2.5 PROSPECTIVE | 第199页 |
| Ⅴ.2.6 FIGURES AND LEGENDS | 第199-208页 |
| GENERAL CONCLUSION | 第208-213页 |
| REFERENCES | 第213-230页 |
