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Endophytic Sphingomonas Hylomeconis Sp. Nov., Isolated from The Stem of Hylomecon Japonica

Abstract第7-8页
摘要第9-12页
Chapter 1. Introduction第12-20页
    1.1 Importance of Sphingomonas第12页
    1.2 Significance of endophytes第12-15页
    1.3 Effect of Climate on Endophytic Population第15页
    1.4 Molecular Studies of Endophytes第15页
    1.5 Applications and roles of Endophytes第15-16页
    1.6 Pigment Production第16页
    1.7 Enzyme Production第16-17页
    1.8 Antimicrobial Activity第17页
    1.9 Different Sources of Bioactives and Novel Compounds第17页
    1.10 Biocontrol Agents第17-18页
    1.11 Multiple Roles of Endophytes第18页
    1.12 Importance of Endophytes in Tissue Culture第18-20页
Chapter 2: Materials and Methods第20-26页
    2.1 Sampling of wild medicinal herbs第20页
    2.2 Surface sterilization of plant samples第20页
    2.3 Isolation of endophytic bacteria第20页
    2.4 Genomic DNA extraction and 16S r RNA gene sequence analysis第20-21页
    2.5 Evaluation of antimicrobial activity第21-22页
    2.6 Detection and analysis of PKS and NRPS gene fragments第22-23页
    2.7 Nucleotide sequence accession numbers第23页
    2.8 16S r RNA gene sequencing of GZJT-2T and phylogenetic analysis第23页
    2.9 Determination of G+C content of genomic DNA and DNA–DNA hybridization第23-24页
    2.10 Morphological, physiological and biochemical characterization第24页
    2.11 Chemotaxonomic characterization of strain GZJT-2T第24-26页
Chapter 3: Results第26-48页
    3.1 The diversity and biosynthetic potential of endophytic bacteria isolated fromtraditional herbs of taibai mountain, North-West China第26-38页
        3.1.1 The fetures of the five traditional medicinal herbs collected from TaibaiMountain第26页
        3.1.2 Isolation, identification and phylogenetic analysis of endophytic bacteria第26-32页
        3.1.3 Evaluation of the antimicrobial activity第32-34页
        3.1.4 Screening of PKS and NRPS gene fragments and phylogenetic analysis第34-38页
    3.2 Identification of the endophytic novel species Sphingomonas hylomeconis sp. nov.第38-48页
        3.2.1 Phylogenetic analysis第39-40页
        3.2.2 Genomic DNA G+C content of GZJT-2T and DNA–DNA hybridization第40-41页
        3.2.3 Morphological, physiological and biochemical characteristics第41-43页
        3.2.4 Cheomtaxonomic characteristics第43-46页
        3.2.5 Taxonomic Conclusion第46页
        3.2.6 Description of Sphingomonas hylomeconis sp. nov第46-48页
Chapter 4 Discussions第48-55页
References第55-64页
ABBREVIATIONS第64-65页
Acknowledgement第65页

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