| 摘要 | 第7-10页 |
| Abstract | 第10-13页 |
| List of Abbreviations | 第14-16页 |
| Chapter 1 Introduction | 第16-20页 |
| 1.1 Ultrastructure of oviduct in relation with its secretions | 第16-17页 |
| 1.2 Ultrastructure of efferent ductules in male reproductive tract | 第17-18页 |
| 1.3 Research goals | 第18-20页 |
| Chapter 2 Literature Review | 第20-34页 |
| Part-Ⅰ Introduction to Experimental Animal | 第20-22页 |
| 1.1 Introduction to Chinese soft- shelled turtle Pelodiscus sinensis | 第20-22页 |
| Part Ⅱ Introduction to Reproductive Tract | 第22-24页 |
| 2.1 The female reproductive tract of the Chinese soft- shelled turtle | 第22-23页 |
| 2.2 The male reproductive tract of Chinese soft-shelled turtle | 第23-24页 |
| Part-Ⅲ Oviduct secretions and Efferent ductules | 第24-28页 |
| 3.1 Physiological role of oviduct secretions | 第24-27页 |
| 3.2 Ultrastructure of efferent ductules | 第27-28页 |
| References | 第28-34页 |
| Chapter 3 (Experiment 1) Novel cellular evidence of oviduct secretions in the Chinesesoft-shelled turtle Pelodiscus sinensis | 第34-54页 |
| 1. Introduction | 第34-36页 |
| 2. Materials and Methods | 第36-37页 |
| 2.1 Experimental Animals | 第36页 |
| 2.2 Ethical Approval | 第36页 |
| 2.3 Sample collection and Light Microscopy | 第36页 |
| 2.4 Sperm Staining | 第36页 |
| 2.5 Sample collection and Transmission Electron Microscopy (TEM) | 第36-37页 |
| 3. Results | 第37-48页 |
| 3.1 Oviduct ultrastructure | 第37-38页 |
| 3.2 Ultrastructure of the oviduct epithelium related to secretion | 第38-39页 |
| 3.3 Ultrastructure of oviduct glands | 第39-48页 |
| 4. Discussion | 第48-50页 |
| 4.1 Ultrastructure of the oviduct in relation to the unique secretions of epithelial cells | 第48页 |
| 4.2 Glandular secretions | 第48-50页 |
| 4.3 Sperm storage and oviduct secretions | 第50页 |
| 5. Conclusion | 第50-51页 |
| References | 第51-54页 |
| Chapter 4 (Experiment 2) Epithelial and glandular evidence of exosomes andsecretions in the oviduct of Chinese soft- shelled turtle Pelodiscus sinensis | 第54-72页 |
| 1. Introduction | 第54-56页 |
| 2. Material and Methods | 第56-57页 |
| 2.1 Animals | 第56页 |
| 2.2 Immunohistochemistry | 第56-57页 |
| 2.3 Transmission Electron Microscopy (TEM) | 第57页 |
| 3. Results | 第57-64页 |
| 3.1 Immunohistochemistry | 第57-58页 |
| 3.2 Detection of exosomes through transmission electron microscopy | 第58-64页 |
| 4. Discussion | 第64-68页 |
| References | 第68-72页 |
| Chapter 5 (Experiment 3) Comparative study of the ultrastructure and secretion ofoviduct and SSTs between the turtle and hen | 第72-88页 |
| 1. Introduction | 第73-74页 |
| 2. Materials and Methods | 第74-75页 |
| 2.1 Animal Use | 第74页 |
| 2.2 Samples Collection | 第74-75页 |
| 2.3 Light microscopy | 第75页 |
| 2.4 Transmission Electron Microscopy | 第75页 |
| 3 Results | 第75-82页 |
| 3.1 Distribution of SSTs in turtle oviduct | 第75页 |
| 3.2 Ultrastructure of the epithelium in the isthmus of turtle oviduct | 第75-76页 |
| 3.3 Ultrastructure of the epithelium in UVJ of hen oviduct | 第76-77页 |
| 3.4 Ultrastructure of SSTs and its unique secretions of hen | 第77页 |
| 3.5 Comparison of ultrastructure and secretory cytology of hen and turtle | 第77-82页 |
| 4. Discussion | 第82-84页 |
| 5. Conclusion | 第84-85页 |
| References | 第85-88页 |
| Chapter 6 (Experiment 4) Morphological and ultrastructural study of the efferentductules in the Chinese soft shelled turtle Pelodiscus sinensis | 第88-104页 |
| 1. Introduction | 第88-90页 |
| 2. Materials and Methods | 第90-91页 |
| 2.1 Experimental animals | 第90页 |
| 2.2 Light Microscopy | 第90页 |
| 2.3 Transmission Electron Microscopy (TEM) | 第90-91页 |
| 3. Results | 第91-100页 |
| 3.1 Light microscopic observations | 第91-92页 |
| 3.2 Electron microscopy observations | 第92-100页 |
| 4. Discussion | 第100-101页 |
| 5. Conclusion | 第101-102页 |
| References | 第102-104页 |
| Summary | 第104-106页 |
| Conclusion | 第106-108页 |
| Innovations | 第108-110页 |
| Future Research Directions | 第110-112页 |
| Publications | 第112-114页 |
| ACKNOWLEDGEMENTS | 第114页 |
