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Genetic Analysis of Salt Tolerance in Cucumber(Cucumis Sativus L.)

ABSTRACT第13-16页
摘要第17-21页
CHAPTER ONE: GENERAL INTRODUCTION AND LITERTURE REVIEW第21-37页
    1.1 Background information第21-22页
    1.2 Causes of soil salinity第22页
    1.3 Effects of salinity on plant growth and development第22-23页
    1.4 Mechanisms of salt tolerance in glycophytes第23-26页
        1.4.1 Ion exclusion and inclusion第24-25页
        1.4.2 Antioxidant defense第25页
        1.4.3 Accumulation of compatible solutes第25-26页
    1.5 Factors affecting salinity tolerance of crops第26页
    1.6 Mitigation of adverse soil salinity effects on plant growth第26-30页
        1.6.1 Application of exogenous plant growth regulators第27-28页
        1.6.2 Mineral nutrition and salinity tolerance第28-29页
        1.6.3 Role of grafting on salt tolerance in cucumber第29页
        1.6.4 Improving salinity tolerance of crops through transgenic approaches第29-30页
        1.6.5 Improving salinity tolerance through conventional breeding第30页
    1.7 Genetics of salinity tolerance in glycophtytes第30-35页
        1.7.1 Selection criteria for salinity tolerance in glycophytes第31页
        1.7.2 Screening system for salinity tolerance第31-32页
        1.7.3 Molecular studies of salinity tolerance in glycophytes第32-34页
        1.7.4 Proteomics of salt tolerance in cucumber第34-35页
    1.8 The problem statement第35页
    1.9 Justification of the study第35-36页
    1.10 Aims第36页
    1.11 Objectives第36页
    1.12 Limitations第36-37页
CHAPTER TWO: EVALUATION OF SALT TOLERANCE OF CUCUMBER AT SEEDLING AND GERMINATION STAGES第37-57页
    2.1 Introduction第37-39页
    2.2 Materials and Methods第39-43页
        2.2.1 Seedling experiment第39页
        2.2.2 Plant materials and treatments第39-41页
        2.2.3 Plant growth and survival rate第41页
        2.2.4 Cell membrane stability第41页
        2.2.5 Gas exchange characteristics第41-42页
        2.2.6 Plant pigment determination第42页
        2.2.7 Mineral distribution第42页
        2.2.8 Germination experiment第42-43页
        2.2.9 Statistical Analysis第43页
    2.3 Results第43-51页
        2.3.1 Growth and survival rate第43-46页
        2.3.2 Cell membrane permeability第46-47页
        2.3.3 Gas exchange characteristics第47-48页
        2.3.4 Leaf pigment and mineral distribution第48-50页
        2.3.5 Germination response of cucumber genotypes to NaCl salinity第50-51页
    2.4 Discussion第51-56页
    2.5 Conclusions第56-57页
CHAPTER THREE: HERITABILITY AND GENE EFFECTS FOR SALINITY TOLERANCE IN CUCUMBER ESTIMATED BY GENERATION MEAN ANALYSIS第57-69页
    3.1 Introduction第57-59页
    3.2 Materials and methods第59-61页
        3.2.1 Experimental material第59页
        3.2.2 Experimental design and treatment第59-60页
        3.2.3 Evaluation of salt tolerance第60页
        3.2.4 Data analysis第60-61页
        3.2.5 Phenotypic and genotypic correlation第61页
    3.3 Results第61-66页
        3.3.1 Mean values of trait of parental lines and their progeny under salinity第61-62页
        3.3.2 Inheritance of salinity tolerance in cucumber第62-63页
        3.3.3 Gene effects on salt tolerance (TOL), relative leaf number (RLN14), leaf area(LA) and vine length (VL) of cucumber cross 11411S x 11439S第63-64页
        3.3.4 Dominance ratio, heterosis, potence ratio and heritability of various traits of cucumber under 80 mM NaCl stress第64-65页
        3.3.5 Trait correlation under salinity stress第65-66页
    3.4 Discussion第66-68页
    3.5 Conclusion第68-69页
CHAPTER FOUR: QUANTITATIVE TRAIT ANALYSIS OF SALT TOLERANCE IN CUCUMBER第69-83页
    4.1 Introduction第70-72页
    4.2 Materials and methods第72-76页
        4.2.1 Development of mapping population for QTL analysis第72页
        4.2.2 Phenotypic evaluation of salinity tolerance第72-74页
        4.2.3 DNA extraction第74页
        4.2.4 SSR marker and QTL analyses第74-75页
        4.2.5 Genetic Linkage Map construction and QTL analysis第75页
        4.2.6 Statistical analysis第75-76页
    4.3 Results第76-80页
        4.3.1 Salinity tolerance of parental and F_(2:3) lines第76-77页
        4.3.2 Phenotypic correlations第77-78页
        4.3.3 QTLs associated with salinity tolerance traits第78-80页
    4.4 Discussion第80-82页
    4.5 Conclusions第82-83页
CHAPTER FIVE: GENERAL DISCUSSION第83-87页
CONCLUSIONS第87-89页
NEW KNOWLEDGE第89-91页
REFERENCES第91-107页
ABOUT THE AUTHOR第107-109页
PUBLICATIONS第109-111页
ACKNOWLEGDGEMENTS第111-112页

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